Possible Metastatic Stage-Dependent ILC2 Activation Induces Differential Functions of MDSCs through IL-13/IL-13Rα1 Signaling during the Progression of Breast Cancer Lung Metastasis
Breast cancer is the most common cancer in women worldwide, and lung metastasis is one of the most frequent distant metastases. When breast cancer metastasizes to the lung, group 2 innate lymphoid cells (ILC2s) are thought to promote tumor growth via the activation of myeloid-derived suppressor cell...
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MDPI AG
2022-07-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/13/3267 |
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| author | Atsushi Ito Yuichi Akama Naoko Satoh-Takayama Kanako Saito Takuma Kato Eiji Kawamoto Arong Gaowa Eun Jeong Park Motoshi Takao Motomu Shimaoka |
| author_facet | Atsushi Ito Yuichi Akama Naoko Satoh-Takayama Kanako Saito Takuma Kato Eiji Kawamoto Arong Gaowa Eun Jeong Park Motoshi Takao Motomu Shimaoka |
| author_sort | Atsushi Ito |
| collection | DOAJ |
| description | Breast cancer is the most common cancer in women worldwide, and lung metastasis is one of the most frequent distant metastases. When breast cancer metastasizes to the lung, group 2 innate lymphoid cells (ILC2s) are thought to promote tumor growth via the activation of myeloid-derived suppressor cells (MDSCs), which are known to negatively regulate anticancer immune responses. However, it remains to be elucidated exactly how this ILC2–MDSC interaction is involved in tumor growth during metastases formation. Using a 4T1/LM4 breast cancer mouse model, we found that ILC2s were activated in both the micro- and macrometastatic regions, suggesting sustained activation throughout the metastatic cascades via IL-33/ST2 signaling. Consistent with IL-13 secretion from activated ILC2s, the frequencies of polymorphonuclear (PMN)- and monocytic (M)-MDSCs were also significantly elevated during the progression from micro- to macrometastatic cancer. However, the effects of ILC2-induced MDSC functionality on the microenvironment differed in a metastatic-stage-specific manner. Our findings indicate that ILC2s may induce the immunosuppressive functions of MDSCs during the later stages of metastasis. Concomitantly, ILC2 may instigate extracellular matrix remodeling by PMN-MDSC activation during the early stages of metastasis. These metastatic-stage-specific changes may contribute to metastatic tumor growth in the microenvironment of breast cancer lung metastasis. |
| first_indexed | 2024-03-09T22:02:14Z |
| format | Article |
| id | doaj.art-0fb5883e3fe143b5b813b87b5c721915 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T22:02:14Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Cancers |
| spelling | doaj.art-0fb5883e3fe143b5b813b87b5c7219152023-11-23T19:47:08ZengMDPI AGCancers2072-66942022-07-011413326710.3390/cancers14133267Possible Metastatic Stage-Dependent ILC2 Activation Induces Differential Functions of MDSCs through IL-13/IL-13Rα1 Signaling during the Progression of Breast Cancer Lung MetastasisAtsushi Ito0Yuichi Akama1Naoko Satoh-Takayama2Kanako Saito3Takuma Kato4Eiji Kawamoto5Arong Gaowa6Eun Jeong Park7Motoshi Takao8Motomu Shimaoka9Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, JapanLaboratory for Intestinal Ecosystem, Center for Integrative Medical Sciences, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Kanagawa, JapanDepartment of Hematology and Oncology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, JapanDepartment of Cellular and Molecular Immunology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, JapanDepartment of Thoracic and Cardiovascular Surgery, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, JapanBreast cancer is the most common cancer in women worldwide, and lung metastasis is one of the most frequent distant metastases. When breast cancer metastasizes to the lung, group 2 innate lymphoid cells (ILC2s) are thought to promote tumor growth via the activation of myeloid-derived suppressor cells (MDSCs), which are known to negatively regulate anticancer immune responses. However, it remains to be elucidated exactly how this ILC2–MDSC interaction is involved in tumor growth during metastases formation. Using a 4T1/LM4 breast cancer mouse model, we found that ILC2s were activated in both the micro- and macrometastatic regions, suggesting sustained activation throughout the metastatic cascades via IL-33/ST2 signaling. Consistent with IL-13 secretion from activated ILC2s, the frequencies of polymorphonuclear (PMN)- and monocytic (M)-MDSCs were also significantly elevated during the progression from micro- to macrometastatic cancer. However, the effects of ILC2-induced MDSC functionality on the microenvironment differed in a metastatic-stage-specific manner. Our findings indicate that ILC2s may induce the immunosuppressive functions of MDSCs during the later stages of metastasis. Concomitantly, ILC2 may instigate extracellular matrix remodeling by PMN-MDSC activation during the early stages of metastasis. These metastatic-stage-specific changes may contribute to metastatic tumor growth in the microenvironment of breast cancer lung metastasis.https://www.mdpi.com/2072-6694/14/13/3267group 2 innate lymphoid cellsbreast cancerlung metastasismicro- and macrometastasistumor microenvironmentmyeloid-derived suppressor cells |
| spellingShingle | Atsushi Ito Yuichi Akama Naoko Satoh-Takayama Kanako Saito Takuma Kato Eiji Kawamoto Arong Gaowa Eun Jeong Park Motoshi Takao Motomu Shimaoka Possible Metastatic Stage-Dependent ILC2 Activation Induces Differential Functions of MDSCs through IL-13/IL-13Rα1 Signaling during the Progression of Breast Cancer Lung Metastasis Cancers group 2 innate lymphoid cells breast cancer lung metastasis micro- and macrometastasis tumor microenvironment myeloid-derived suppressor cells |
| title | Possible Metastatic Stage-Dependent ILC2 Activation Induces Differential Functions of MDSCs through IL-13/IL-13Rα1 Signaling during the Progression of Breast Cancer Lung Metastasis |
| title_full | Possible Metastatic Stage-Dependent ILC2 Activation Induces Differential Functions of MDSCs through IL-13/IL-13Rα1 Signaling during the Progression of Breast Cancer Lung Metastasis |
| title_fullStr | Possible Metastatic Stage-Dependent ILC2 Activation Induces Differential Functions of MDSCs through IL-13/IL-13Rα1 Signaling during the Progression of Breast Cancer Lung Metastasis |
| title_full_unstemmed | Possible Metastatic Stage-Dependent ILC2 Activation Induces Differential Functions of MDSCs through IL-13/IL-13Rα1 Signaling during the Progression of Breast Cancer Lung Metastasis |
| title_short | Possible Metastatic Stage-Dependent ILC2 Activation Induces Differential Functions of MDSCs through IL-13/IL-13Rα1 Signaling during the Progression of Breast Cancer Lung Metastasis |
| title_sort | possible metastatic stage dependent ilc2 activation induces differential functions of mdscs through il 13 il 13rα1 signaling during the progression of breast cancer lung metastasis |
| topic | group 2 innate lymphoid cells breast cancer lung metastasis micro- and macrometastasis tumor microenvironment myeloid-derived suppressor cells |
| url | https://www.mdpi.com/2072-6694/14/13/3267 |
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