GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against <i>Colletotrichum gloeosporioides</i> Infection
GDSL esterases/lipases are a subclass of lipolytic enzymes that play critical roles in plant growth and development, stress response, and pathogen defense. However, the GDSL esterase/lipase genes involved in the pathogen response of apple remain to be identified and characterized. Thus, in this stud...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/12/10343 |
| _version_ | 1797594255391195136 |
|---|---|
| author | Zhirui Ji Meiyu Wang Shuwu Zhang Yinan Du Jialin Cong Haifeng Yan Haimeng Guo Bingliang Xu Zongshan Zhou |
| author_facet | Zhirui Ji Meiyu Wang Shuwu Zhang Yinan Du Jialin Cong Haifeng Yan Haimeng Guo Bingliang Xu Zongshan Zhou |
| author_sort | Zhirui Ji |
| collection | DOAJ |
| description | GDSL esterases/lipases are a subclass of lipolytic enzymes that play critical roles in plant growth and development, stress response, and pathogen defense. However, the GDSL esterase/lipase genes involved in the pathogen response of apple remain to be identified and characterized. Thus, in this study, we aimed to analyze the phenotypic difference between the resistant variety, Fuji, and susceptible variety, Gala, during infection with <i>C. gloeosporioides</i>, screen for anti-disease-associated proteins in Fuji leaves, and elucidate the underlying mechanisms. The results showed that GDSL esterase/lipase protein GELP1 contributed to <i>C. gloeosporioides</i> infection defense in apple. During <i>C. gloeosporioides</i> infection, <i>GELP1</i> expression was significantly upregulated in Fuji. Fuji leaves exhibited a highly resistant phenotype compared with Gala leaves. The formation of infection hyphae of <i>C. gloeosporioides</i> was inhibited in Fuji. Moreover, recombinant His:GELP1 protein suppressed hyphal formation during infection in vitro. Transient expression in <i>Nicotiana benthamiana</i> showed that GELP1-eGFP localized to the endoplasmic reticulum and chloroplasts. GELP1 overexpression in GL-3 plants increased resistance to <i>C. gloeosporioides</i>. <i>MdWRKY15</i> expression was upregulated in the transgenic lines. Notably, GELP1 transcript levels were elevated in GL-3 after salicylic acid treatment. These results suggest that GELP1 increases apple resistance to <i>C. gloeosporioides</i> by indirectly regulating salicylic acid biosynthesis. |
| first_indexed | 2024-03-11T02:20:03Z |
| format | Article |
| id | doaj.art-0fb6d7e308084477aade8b1feeea638f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:20:03Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0fb6d7e308084477aade8b1feeea638f2023-11-18T10:52:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124121034310.3390/ijms241210343GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against <i>Colletotrichum gloeosporioides</i> InfectionZhirui Ji0Meiyu Wang1Shuwu Zhang2Yinan Du3Jialin Cong4Haifeng Yan5Haimeng Guo6Bingliang Xu7Zongshan Zhou8College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaCollege of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaCollege of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaGDSL esterases/lipases are a subclass of lipolytic enzymes that play critical roles in plant growth and development, stress response, and pathogen defense. However, the GDSL esterase/lipase genes involved in the pathogen response of apple remain to be identified and characterized. Thus, in this study, we aimed to analyze the phenotypic difference between the resistant variety, Fuji, and susceptible variety, Gala, during infection with <i>C. gloeosporioides</i>, screen for anti-disease-associated proteins in Fuji leaves, and elucidate the underlying mechanisms. The results showed that GDSL esterase/lipase protein GELP1 contributed to <i>C. gloeosporioides</i> infection defense in apple. During <i>C. gloeosporioides</i> infection, <i>GELP1</i> expression was significantly upregulated in Fuji. Fuji leaves exhibited a highly resistant phenotype compared with Gala leaves. The formation of infection hyphae of <i>C. gloeosporioides</i> was inhibited in Fuji. Moreover, recombinant His:GELP1 protein suppressed hyphal formation during infection in vitro. Transient expression in <i>Nicotiana benthamiana</i> showed that GELP1-eGFP localized to the endoplasmic reticulum and chloroplasts. GELP1 overexpression in GL-3 plants increased resistance to <i>C. gloeosporioides</i>. <i>MdWRKY15</i> expression was upregulated in the transgenic lines. Notably, GELP1 transcript levels were elevated in GL-3 after salicylic acid treatment. These results suggest that GELP1 increases apple resistance to <i>C. gloeosporioides</i> by indirectly regulating salicylic acid biosynthesis.https://www.mdpi.com/1422-0067/24/12/10343GDSL esterases/lipasesFujiresistanceGlomerella leaf spot |
| spellingShingle | Zhirui Ji Meiyu Wang Shuwu Zhang Yinan Du Jialin Cong Haifeng Yan Haimeng Guo Bingliang Xu Zongshan Zhou GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against <i>Colletotrichum gloeosporioides</i> Infection International Journal of Molecular Sciences GDSL esterases/lipases Fuji resistance Glomerella leaf spot |
| title | GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against <i>Colletotrichum gloeosporioides</i> Infection |
| title_full | GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against <i>Colletotrichum gloeosporioides</i> Infection |
| title_fullStr | GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against <i>Colletotrichum gloeosporioides</i> Infection |
| title_full_unstemmed | GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against <i>Colletotrichum gloeosporioides</i> Infection |
| title_short | GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against <i>Colletotrichum gloeosporioides</i> Infection |
| title_sort | gdsl esterase lipase gelp1 involved in the defense of apple leaves against i colletotrichum gloeosporioides i infection |
| topic | GDSL esterases/lipases Fuji resistance Glomerella leaf spot |
| url | https://www.mdpi.com/1422-0067/24/12/10343 |
| work_keys_str_mv | AT zhiruiji gdslesteraselipasegelp1involvedinthedefenseofappleleavesagainsticolletotrichumgloeosporioidesiinfection AT meiyuwang gdslesteraselipasegelp1involvedinthedefenseofappleleavesagainsticolletotrichumgloeosporioidesiinfection AT shuwuzhang gdslesteraselipasegelp1involvedinthedefenseofappleleavesagainsticolletotrichumgloeosporioidesiinfection AT yinandu gdslesteraselipasegelp1involvedinthedefenseofappleleavesagainsticolletotrichumgloeosporioidesiinfection AT jialincong gdslesteraselipasegelp1involvedinthedefenseofappleleavesagainsticolletotrichumgloeosporioidesiinfection AT haifengyan gdslesteraselipasegelp1involvedinthedefenseofappleleavesagainsticolletotrichumgloeosporioidesiinfection AT haimengguo gdslesteraselipasegelp1involvedinthedefenseofappleleavesagainsticolletotrichumgloeosporioidesiinfection AT bingliangxu gdslesteraselipasegelp1involvedinthedefenseofappleleavesagainsticolletotrichumgloeosporioidesiinfection AT zongshanzhou gdslesteraselipasegelp1involvedinthedefenseofappleleavesagainsticolletotrichumgloeosporioidesiinfection |