Altered serum levels of cytokines in patients with myasthenia gravis
Background: Myasthenia gravis (MG) is an autoimmune disease characterized by generalized skeletal muscle contraction weakness due to autoantibodies targeting neural-muscular junctions. Here, we investigated the relationship between key cytokines and MG type, disease course, antibodies, and comorbidi...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023109534 |
| _version_ | 1797336942595014656 |
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| author | Shu-Li Wei Chun-Lin Yang Wei-Yue Si Jing Dong Xue-Lu Zhao Peng Zhang Heng Li Cong-Cong Wang Min Zhang Xiao-Li Li Rui-Sheng Duan |
| author_facet | Shu-Li Wei Chun-Lin Yang Wei-Yue Si Jing Dong Xue-Lu Zhao Peng Zhang Heng Li Cong-Cong Wang Min Zhang Xiao-Li Li Rui-Sheng Duan |
| author_sort | Shu-Li Wei |
| collection | DOAJ |
| description | Background: Myasthenia gravis (MG) is an autoimmune disease characterized by generalized skeletal muscle contraction weakness due to autoantibodies targeting neural-muscular junctions. Here, we investigated the relationship between key cytokines and MG type, disease course, antibodies, and comorbidities. Method: Cytokine levels in serum samples collected from MG (n = 45) and healthy control (HC, n = 38) patients from January 2020 to June 2022 were quantified via flow cytometry. Results: Levels of IL-6 were higher in the MG group versus healthy individuals (p = 0.026) and in patients with generalized versus ocular MG (p = 0.019). IL-6 levels were positively correlated with QMG score. In patients with MG with both AChR and Titin antibodies, serum levels of sFas and granulysin were higher than in those with AChR alone (p = 0.036, and p = 0.028, respectively). LOMG had a reduction in serum levels of IL-2 compared to EOMG (p = 0.036). LOMG patients with diabetes had lower serum levels of IL-2, IL-4, and IFN-γ (p = 0.044, p = 0.038, and p = 0.047, respectively) versus those without diabetes. sFas in the MG with Abnormal thymus were reduced compared to those in MG with Normal thymus (p = 0.008). Conclusions: This study revealed a positive correlation between IL-6 level and MG status. Serum cytokine levels of the AChR + Titin MG group differed from those of the AChR group. LOMG had a lower IL-2 level. Comorbidities affect some cytokines in peripheral blood in MG serum. |
| first_indexed | 2024-03-08T09:02:10Z |
| format | Article |
| id | doaj.art-0fb749b8ac854f32a0f19e9513af1b18 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T09:02:10Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-0fb749b8ac854f32a0f19e9513af1b182024-02-01T06:33:18ZengElsevierHeliyon2405-84402024-01-01101e23745Altered serum levels of cytokines in patients with myasthenia gravisShu-Li Wei0Chun-Lin Yang1Wei-Yue Si2Jing Dong3Xue-Lu Zhao4Peng Zhang5Heng Li6Cong-Cong Wang7Min Zhang8Xiao-Li Li9Rui-Sheng Duan10Department of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, PR ChinaDepartment of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR China; Shandong Institute of Neuroimmunology, Jinan 250014, PR China; Shandong Provincial Medicine and Health Key Laboratory of Neuroimmunology, Jinan 250014, PR ChinaDepartment of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, PR ChinaDepartment of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR ChinaDepartment of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR ChinaDepartment of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR China; Shandong Institute of Neuroimmunology, Jinan 250014, PR China; Shandong Provincial Medicine and Health Key Laboratory of Neuroimmunology, Jinan 250014, PR ChinaDepartment of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR China; Shandong Institute of Neuroimmunology, Jinan 250014, PR China; Shandong Provincial Medicine and Health Key Laboratory of Neuroimmunology, Jinan 250014, PR ChinaDepartment of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR China; Shandong Institute of Neuroimmunology, Jinan 250014, PR China; Shandong Provincial Medicine and Health Key Laboratory of Neuroimmunology, Jinan 250014, PR ChinaDepartment of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR China; Shandong Institute of Neuroimmunology, Jinan 250014, PR China; Shandong Provincial Medicine and Health Key Laboratory of Neuroimmunology, Jinan 250014, PR ChinaDepartment of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR China; Shandong Institute of Neuroimmunology, Jinan 250014, PR China; Shandong Provincial Medicine and Health Key Laboratory of Neuroimmunology, Jinan 250014, PR China; Corresponding author. Department of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR China.Department of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, PR China; Department of Neurology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, PR China; Shandong Institute of Neuroimmunology, Jinan 250014, PR China; Shandong Provincial Medicine and Health Key Laboratory of Neuroimmunology, Jinan 250014, PR China; Corresponding author. Department of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, PR China.Background: Myasthenia gravis (MG) is an autoimmune disease characterized by generalized skeletal muscle contraction weakness due to autoantibodies targeting neural-muscular junctions. Here, we investigated the relationship between key cytokines and MG type, disease course, antibodies, and comorbidities. Method: Cytokine levels in serum samples collected from MG (n = 45) and healthy control (HC, n = 38) patients from January 2020 to June 2022 were quantified via flow cytometry. Results: Levels of IL-6 were higher in the MG group versus healthy individuals (p = 0.026) and in patients with generalized versus ocular MG (p = 0.019). IL-6 levels were positively correlated with QMG score. In patients with MG with both AChR and Titin antibodies, serum levels of sFas and granulysin were higher than in those with AChR alone (p = 0.036, and p = 0.028, respectively). LOMG had a reduction in serum levels of IL-2 compared to EOMG (p = 0.036). LOMG patients with diabetes had lower serum levels of IL-2, IL-4, and IFN-γ (p = 0.044, p = 0.038, and p = 0.047, respectively) versus those without diabetes. sFas in the MG with Abnormal thymus were reduced compared to those in MG with Normal thymus (p = 0.008). Conclusions: This study revealed a positive correlation between IL-6 level and MG status. Serum cytokine levels of the AChR + Titin MG group differed from those of the AChR group. LOMG had a lower IL-2 level. Comorbidities affect some cytokines in peripheral blood in MG serum.http://www.sciencedirect.com/science/article/pii/S2405844023109534Myasthenia gravisCytokineTitin |
| spellingShingle | Shu-Li Wei Chun-Lin Yang Wei-Yue Si Jing Dong Xue-Lu Zhao Peng Zhang Heng Li Cong-Cong Wang Min Zhang Xiao-Li Li Rui-Sheng Duan Altered serum levels of cytokines in patients with myasthenia gravis Heliyon Myasthenia gravis Cytokine Titin |
| title | Altered serum levels of cytokines in patients with myasthenia gravis |
| title_full | Altered serum levels of cytokines in patients with myasthenia gravis |
| title_fullStr | Altered serum levels of cytokines in patients with myasthenia gravis |
| title_full_unstemmed | Altered serum levels of cytokines in patients with myasthenia gravis |
| title_short | Altered serum levels of cytokines in patients with myasthenia gravis |
| title_sort | altered serum levels of cytokines in patients with myasthenia gravis |
| topic | Myasthenia gravis Cytokine Titin |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023109534 |
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