Fluorescent Imaging for Cysteine Detection In Vivo with High Selectivity
Abstract As an essential amino acid, cysteine is involved in various biosynthetic and metabolic processes, such as protein synthesis, hormone synthesis, and redox homeostatic maintenance. Inordinate cysteine levels are often associated with serious diseases. Thus, designing and synthesizing a novel...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2019-03-01
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| Series: | ChemistryOpen |
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| Online Access: | https://doi.org/10.1002/open.201900045 |
| _version_ | 1797771136713359360 |
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| author | Zhuwen Duan Youming Zhu Yuyun Yang Zixu He Prof. Ju Liu Prof. Ping Li Dr. Hui Wang Prof. Bo Tang |
| author_facet | Zhuwen Duan Youming Zhu Yuyun Yang Zixu He Prof. Ju Liu Prof. Ping Li Dr. Hui Wang Prof. Bo Tang |
| author_sort | Zhuwen Duan |
| collection | DOAJ |
| description | Abstract As an essential amino acid, cysteine is involved in various biosynthetic and metabolic processes, such as protein synthesis, hormone synthesis, and redox homeostatic maintenance. Inordinate cysteine levels are often associated with serious diseases. Thus, designing and synthesizing a novel fluorescent probe for determining the concentration of cellular cysteine, which could indirectly monitor the prevalence of these diseases, is essential. We developed a florescence probe P−Cy with good sensitivity for cysteine detection in vivo. P−Cy only exhibited good response toward cysteine but did not show response toward other biothiols, such as homocysteine (Hcy) and glutathione (GSH). In this study, we used P−Cy by successfully imaging cellular endogenous and exogenous cysteine levels. Furthermore, P−Cy was also performed in mice to detect cysteine level, indicating that P−Cy is a powerful tool for cysteine detection in situ. |
| first_indexed | 2024-03-12T21:33:10Z |
| format | Article |
| id | doaj.art-0fc546e344c743d7839d6a3ba406219b |
| institution | Directory Open Access Journal |
| issn | 2191-1363 |
| language | English |
| last_indexed | 2024-03-12T21:33:10Z |
| publishDate | 2019-03-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemistryOpen |
| spelling | doaj.art-0fc546e344c743d7839d6a3ba406219b2023-07-27T14:18:47ZengWiley-VCHChemistryOpen2191-13632019-03-018331632010.1002/open.201900045Fluorescent Imaging for Cysteine Detection In Vivo with High SelectivityZhuwen Duan0Youming Zhu1Yuyun Yang2Zixu He3Prof. Ju Liu4Prof. Ping Li5Dr. Hui Wang6Prof. Bo Tang7College of Chemistry, Chemical Engineering and Materials Science, Institute of Biomedical Sciences, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong normal University Jinan 250014 PR ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Institute of Biomedical Sciences, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong normal University Jinan 250014 PR ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Institute of Biomedical Sciences, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong normal University Jinan 250014 PR ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Institute of Biomedical Sciences, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong normal University Jinan 250014 PR ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Institute of Biomedical Sciences, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong normal University Jinan 250014 PR ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Institute of Biomedical Sciences, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong normal University Jinan 250014 PR ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Institute of Biomedical Sciences, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong normal University Jinan 250014 PR ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Institute of Biomedical Sciences, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong normal University Jinan 250014 PR ChinaAbstract As an essential amino acid, cysteine is involved in various biosynthetic and metabolic processes, such as protein synthesis, hormone synthesis, and redox homeostatic maintenance. Inordinate cysteine levels are often associated with serious diseases. Thus, designing and synthesizing a novel fluorescent probe for determining the concentration of cellular cysteine, which could indirectly monitor the prevalence of these diseases, is essential. We developed a florescence probe P−Cy with good sensitivity for cysteine detection in vivo. P−Cy only exhibited good response toward cysteine but did not show response toward other biothiols, such as homocysteine (Hcy) and glutathione (GSH). In this study, we used P−Cy by successfully imaging cellular endogenous and exogenous cysteine levels. Furthermore, P−Cy was also performed in mice to detect cysteine level, indicating that P−Cy is a powerful tool for cysteine detection in situ.https://doi.org/10.1002/open.201900045cysteinein vivo detectionfluorescence imagingbiothiolprobes |
| spellingShingle | Zhuwen Duan Youming Zhu Yuyun Yang Zixu He Prof. Ju Liu Prof. Ping Li Dr. Hui Wang Prof. Bo Tang Fluorescent Imaging for Cysteine Detection In Vivo with High Selectivity ChemistryOpen cysteine in vivo detection fluorescence imaging biothiol probes |
| title | Fluorescent Imaging for Cysteine Detection In Vivo with High Selectivity |
| title_full | Fluorescent Imaging for Cysteine Detection In Vivo with High Selectivity |
| title_fullStr | Fluorescent Imaging for Cysteine Detection In Vivo with High Selectivity |
| title_full_unstemmed | Fluorescent Imaging for Cysteine Detection In Vivo with High Selectivity |
| title_short | Fluorescent Imaging for Cysteine Detection In Vivo with High Selectivity |
| title_sort | fluorescent imaging for cysteine detection in vivo with high selectivity |
| topic | cysteine in vivo detection fluorescence imaging biothiol probes |
| url | https://doi.org/10.1002/open.201900045 |
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