Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway.
The activation of nuclear factor kappa B (NF-κB) plays a major role in the pathogenesis of a number of inflammatory diseases. In this study, we investigated the anti-inflammatory mechanism of Gelam honey in inflammation induced rats via NF-κB signalling pathway. Rats paw edema was induced by subplan...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3756081?pdf=render |
| _version_ | 1818416063367348224 |
|---|---|
| author | Saba Zuhair Hussein Kamaruddin Mohd Yusoff Suzana Makpol Yasmin Anum Mohd Yusof |
| author_facet | Saba Zuhair Hussein Kamaruddin Mohd Yusoff Suzana Makpol Yasmin Anum Mohd Yusof |
| author_sort | Saba Zuhair Hussein |
| collection | DOAJ |
| description | The activation of nuclear factor kappa B (NF-κB) plays a major role in the pathogenesis of a number of inflammatory diseases. In this study, we investigated the anti-inflammatory mechanism of Gelam honey in inflammation induced rats via NF-κB signalling pathway. Rats paw edema was induced by subplantar injection of 1% carrageenan into the right hind paw. Rats were pre-treated with Gelam honey at different doses (1 or 2 g/kg, p.o.) and NSAID Indomethacin (10 mg/kg, p.o.), in two time points (1 and 7 days). Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues. In addition, Gelam honey inhibited the nuclear translocation and activation of NF-κB and decreased the cytosolic degradation of IκBα dose dependently in inflamed rats paw tissues. The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey. The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α. |
| first_indexed | 2024-12-14T11:44:56Z |
| format | Article |
| id | doaj.art-0fc624ca2bec49f6976bd5e1c442662c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T11:44:56Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0fc624ca2bec49f6976bd5e1c442662c2022-12-21T23:02:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7236510.1371/journal.pone.0072365Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway.Saba Zuhair HusseinKamaruddin Mohd YusoffSuzana MakpolYasmin Anum Mohd YusofThe activation of nuclear factor kappa B (NF-κB) plays a major role in the pathogenesis of a number of inflammatory diseases. In this study, we investigated the anti-inflammatory mechanism of Gelam honey in inflammation induced rats via NF-κB signalling pathway. Rats paw edema was induced by subplantar injection of 1% carrageenan into the right hind paw. Rats were pre-treated with Gelam honey at different doses (1 or 2 g/kg, p.o.) and NSAID Indomethacin (10 mg/kg, p.o.), in two time points (1 and 7 days). Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues. In addition, Gelam honey inhibited the nuclear translocation and activation of NF-κB and decreased the cytosolic degradation of IκBα dose dependently in inflamed rats paw tissues. The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey. The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.http://europepmc.org/articles/PMC3756081?pdf=render |
| spellingShingle | Saba Zuhair Hussein Kamaruddin Mohd Yusoff Suzana Makpol Yasmin Anum Mohd Yusof Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway. PLoS ONE |
| title | Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway. |
| title_full | Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway. |
| title_fullStr | Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway. |
| title_full_unstemmed | Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway. |
| title_short | Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway. |
| title_sort | gelam honey attenuates carrageenan induced rat paw inflammation via nf κb pathway |
| url | http://europepmc.org/articles/PMC3756081?pdf=render |
| work_keys_str_mv | AT sabazuhairhussein gelamhoneyattenuatescarrageenaninducedratpawinflammationvianfkbpathway AT kamaruddinmohdyusoff gelamhoneyattenuatescarrageenaninducedratpawinflammationvianfkbpathway AT suzanamakpol gelamhoneyattenuatescarrageenaninducedratpawinflammationvianfkbpathway AT yasminanummohdyusof gelamhoneyattenuatescarrageenaninducedratpawinflammationvianfkbpathway |