TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles
Among metal nanoparticles, silver nanoparticles are a widely used in various life sectors such as in biomedical applications, air and water purification, food production, cosmetics, garments and in various household products. There are several methods for production of silver nanoparticles. Generall...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2017-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://doi.org/10.1051/e3sconf/20171700015 |
| _version_ | 1818855037369057280 |
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| author | Chojniak Joanna Biedroń Izabela Płaza Grażyna |
| author_facet | Chojniak Joanna Biedroń Izabela Płaza Grażyna |
| author_sort | Chojniak Joanna |
| collection | DOAJ |
| description | Among metal nanoparticles, silver nanoparticles are a widely used in various life sectors such as in biomedical applications, air and water purification, food production, cosmetics, garments and in various household products. There are several methods for production of silver nanoparticles. Generally, silver nanoparticles can be prepared by chemical methods such as chemical reduction and electrochemical techniques, physical methods, and biological methods such as the use of microorganisms. The biological route of synthesis provides a great diversity in choice for its raw materials such as bacteria, algae, fungi and plants. The aim of the study was to evaluate the tetrazolium/formazan test as a method to determine antibiofilm activity of biological synthetized silver nanoparticles. In this study Bacillus subtilis grown on brewery effluent and produced biosurfactant was used for silver nanoparticles (Ag-NPs) synthesis. The culture supernatants were used in synthesis of Ag-NPs. The formation of nanoparticles accompanied by colour changes of the used reaction system was confirmed by UV-vis spectroscopy. The bacteria isolated from the biofilm of water supply system were used in the evaluation of the antibiofilm activity of biologically synthetized Ag-NPs. To compare the results the commonly used crystal violet assay (CV) for biofilm analysis was applied. |
| first_indexed | 2024-12-19T08:02:14Z |
| format | Article |
| id | doaj.art-0fc87d230469414a8fa4203352e05171 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-19T08:02:14Z |
| publishDate | 2017-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-0fc87d230469414a8fa4203352e051712022-12-21T20:29:49ZengEDP SciencesE3S Web of Conferences2267-12422017-01-01170001510.1051/e3sconf/20171700015e3sconf_eko2017_00015TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver NanoparticlesChojniak Joanna0Biedroń Izabela1Płaza Grażyna2Institute for Ecology of Industrial Areas, Microbiology DepartmentInstitute for Ecology of Industrial Areas, Microbiology DepartmentInstitute for Ecology of Industrial Areas, Microbiology DepartmentAmong metal nanoparticles, silver nanoparticles are a widely used in various life sectors such as in biomedical applications, air and water purification, food production, cosmetics, garments and in various household products. There are several methods for production of silver nanoparticles. Generally, silver nanoparticles can be prepared by chemical methods such as chemical reduction and electrochemical techniques, physical methods, and biological methods such as the use of microorganisms. The biological route of synthesis provides a great diversity in choice for its raw materials such as bacteria, algae, fungi and plants. The aim of the study was to evaluate the tetrazolium/formazan test as a method to determine antibiofilm activity of biological synthetized silver nanoparticles. In this study Bacillus subtilis grown on brewery effluent and produced biosurfactant was used for silver nanoparticles (Ag-NPs) synthesis. The culture supernatants were used in synthesis of Ag-NPs. The formation of nanoparticles accompanied by colour changes of the used reaction system was confirmed by UV-vis spectroscopy. The bacteria isolated from the biofilm of water supply system were used in the evaluation of the antibiofilm activity of biologically synthetized Ag-NPs. To compare the results the commonly used crystal violet assay (CV) for biofilm analysis was applied.https://doi.org/10.1051/e3sconf/20171700015 |
| spellingShingle | Chojniak Joanna Biedroń Izabela Płaza Grażyna TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles E3S Web of Conferences |
| title | TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles |
| title_full | TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles |
| title_fullStr | TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles |
| title_full_unstemmed | TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles |
| title_short | TTC- Based Test as an Efficient Method to Determine Antibiofilm Activity of Silver Nanoparticles |
| title_sort | ttc based test as an efficient method to determine antibiofilm activity of silver nanoparticles |
| url | https://doi.org/10.1051/e3sconf/20171700015 |
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