Transcriptome Analysis of Two Tetraploid Potato Varieties under Water-Stress Conditions
Potato (<i>Solanum tuberosum</i> L.) is one of the most important crops worldwide, but due to its sensitivity to drought, its production can be affected by water availability. In this study, the varieties Agria and Zorba were used to determine the expression differences between control a...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/13905 |
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| author | Alba Alvarez-Morezuelas Leire Barandalla Enrique Ritter Jose Ignacio Ruiz de Galarreta |
| author_facet | Alba Alvarez-Morezuelas Leire Barandalla Enrique Ritter Jose Ignacio Ruiz de Galarreta |
| author_sort | Alba Alvarez-Morezuelas |
| collection | DOAJ |
| description | Potato (<i>Solanum tuberosum</i> L.) is one of the most important crops worldwide, but due to its sensitivity to drought, its production can be affected by water availability. In this study, the varieties Agria and Zorba were used to determine the expression differences between control and water-stressed plants. For this purpose, they were sequenced by RNAseq, obtaining around 50 million transcripts for each variety and treatment. When comparing the significant transcripts obtained from control and drought-stressed plants of the Agria variety, we detected 931 genes that were upregulated and 2077 genes that were downregulated under stress conditions. When both treatments were compared in Zorba plants, 735 genes were found to be upregulated and 923 genes were found to be downregulated. Significantly more DEGs were found in the Agria variety, indicating a good stress response of this variety. “Abscisic acid and environmental stress-inducible protein TAS14-like” was the most overexpressed gene under drought conditions in both varieties, but expression differences were also found in numerous transcription factors and heat shock proteins. The principal GO term found was “cellular components”, more specifically related to the cell membrane and the cell wall, but other metabolic pathways such as carbohydrate metabolism and osmotic adjustment were also identified. These results provide valuable information related to the molecular mechanisms of tolerance to water stress in order to establish the basis for breeding new, more tolerant varieties. |
| first_indexed | 2024-03-09T18:17:20Z |
| format | Article |
| id | doaj.art-0fcaa56d93a14f56b894948ff5fe7f9f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:17:20Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0fcaa56d93a14f56b894948ff5fe7f9f2023-11-24T08:35:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221390510.3390/ijms232213905Transcriptome Analysis of Two Tetraploid Potato Varieties under Water-Stress ConditionsAlba Alvarez-Morezuelas0Leire Barandalla1Enrique Ritter2Jose Ignacio Ruiz de Galarreta3NEIKER-Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Campus Agroalimentario de Arkaute, 01192 Arkaute, SpainNEIKER-Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Campus Agroalimentario de Arkaute, 01192 Arkaute, SpainNEIKER-Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Campus Agroalimentario de Arkaute, 01192 Arkaute, SpainNEIKER-Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Campus Agroalimentario de Arkaute, 01192 Arkaute, SpainPotato (<i>Solanum tuberosum</i> L.) is one of the most important crops worldwide, but due to its sensitivity to drought, its production can be affected by water availability. In this study, the varieties Agria and Zorba were used to determine the expression differences between control and water-stressed plants. For this purpose, they were sequenced by RNAseq, obtaining around 50 million transcripts for each variety and treatment. When comparing the significant transcripts obtained from control and drought-stressed plants of the Agria variety, we detected 931 genes that were upregulated and 2077 genes that were downregulated under stress conditions. When both treatments were compared in Zorba plants, 735 genes were found to be upregulated and 923 genes were found to be downregulated. Significantly more DEGs were found in the Agria variety, indicating a good stress response of this variety. “Abscisic acid and environmental stress-inducible protein TAS14-like” was the most overexpressed gene under drought conditions in both varieties, but expression differences were also found in numerous transcription factors and heat shock proteins. The principal GO term found was “cellular components”, more specifically related to the cell membrane and the cell wall, but other metabolic pathways such as carbohydrate metabolism and osmotic adjustment were also identified. These results provide valuable information related to the molecular mechanisms of tolerance to water stress in order to establish the basis for breeding new, more tolerant varieties.https://www.mdpi.com/1422-0067/23/22/13905drought stress<i>Solanum tuberosum</i> L.RNAseqdifferentially expressed genes |
| spellingShingle | Alba Alvarez-Morezuelas Leire Barandalla Enrique Ritter Jose Ignacio Ruiz de Galarreta Transcriptome Analysis of Two Tetraploid Potato Varieties under Water-Stress Conditions International Journal of Molecular Sciences drought stress <i>Solanum tuberosum</i> L. RNAseq differentially expressed genes |
| title | Transcriptome Analysis of Two Tetraploid Potato Varieties under Water-Stress Conditions |
| title_full | Transcriptome Analysis of Two Tetraploid Potato Varieties under Water-Stress Conditions |
| title_fullStr | Transcriptome Analysis of Two Tetraploid Potato Varieties under Water-Stress Conditions |
| title_full_unstemmed | Transcriptome Analysis of Two Tetraploid Potato Varieties under Water-Stress Conditions |
| title_short | Transcriptome Analysis of Two Tetraploid Potato Varieties under Water-Stress Conditions |
| title_sort | transcriptome analysis of two tetraploid potato varieties under water stress conditions |
| topic | drought stress <i>Solanum tuberosum</i> L. RNAseq differentially expressed genes |
| url | https://www.mdpi.com/1422-0067/23/22/13905 |
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