Characterization of a Disease-Suppressive Isolate of <i>Lysobacter enzymogenes</i> with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops
Although synthetic pesticides play a major role in plant protection, their application needs to be reduced because of their negative impact on the environment. This applies also to copper preparations, which are used in organic farming. For this reason, alternatives with less impact on the environme...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/3/682 |
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| author | Christian Drenker Doris El Mazouar Gerrit Bücker Sonja Weißhaupt Eveline Wienke Eckhard Koch Stefan Kunz Annette Reineke Yvonne Rondot Ada Linkies |
| author_facet | Christian Drenker Doris El Mazouar Gerrit Bücker Sonja Weißhaupt Eveline Wienke Eckhard Koch Stefan Kunz Annette Reineke Yvonne Rondot Ada Linkies |
| author_sort | Christian Drenker |
| collection | DOAJ |
| description | Although synthetic pesticides play a major role in plant protection, their application needs to be reduced because of their negative impact on the environment. This applies also to copper preparations, which are used in organic farming. For this reason, alternatives with less impact on the environment are urgently needed. In this context, we evaluated eight isolates of the genus <i>Lysobacter</i> (mainly <i>Lysobacter enzymogenes</i>) for their activity against plant pathogens. In vitro, the investigated <i>Lysobacter</i> isolates showed broad antagonistic activity against several phytopathogenic fungi, oomycetes and bacteria. Enzyme assays revealed diverse activities for the tested isolates. The most promising <i>L. enzymogenes</i> isolate (LEC) was used for further detailed analyses of its efficacy and effective working concentrations. The experiments included in vitro spore and sporangia germination tests and leaf disc assays as well as ad planta growth chamber trials against <i>Alternaria solani</i> and <i>Phytophthora infestans</i> on tomato plants, <i>Pseudoperonospora cubensis</i> on cucumbers and <i>Venturia inaequalis</i> on young potted apple trees. When applied on leaves, dilutions of a culture suspension of LEC had a concentration-dependent, protective effect against the tested pathogens. In all pathosystems tested, the effective concentrations were in the range of 2.5–5% and similarly efficacious to common plant protection agents containing copper hydroxide, wettable sulphur or fenhexamid. Thus, the isolate of <i>L. enzymogenes</i> identified in this study exhibits a broad activity against common plant pathogens and is therefore a promising candidate for the development of a microbial biocontrol agent. |
| first_indexed | 2024-03-11T09:29:04Z |
| format | Article |
| id | doaj.art-0fcb0b188109472590c1d4806a81f3ff |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:29:04Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-0fcb0b188109472590c1d4806a81f3ff2023-11-16T17:45:54ZengMDPI AGPlants2223-77472023-02-0112368210.3390/plants12030682Characterization of a Disease-Suppressive Isolate of <i>Lysobacter enzymogenes</i> with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different CropsChristian Drenker0Doris El Mazouar1Gerrit Bücker2Sonja Weißhaupt3Eveline Wienke4Eckhard Koch5Stefan Kunz6Annette Reineke7Yvonne Rondot8Ada Linkies9Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Biological Control, 69221 Dossenheim, GermanyJulius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Biological Control, 69221 Dossenheim, GermanyJulius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Biological Control, 69221 Dossenheim, GermanyBio-Protect GmbH, 78467 Konstanz, GermanyBio-Protect GmbH, 78467 Konstanz, GermanyJulius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Biological Control, 69221 Dossenheim, GermanyBio-Protect GmbH, 78467 Konstanz, GermanyDepartment of Crop Protection, Hochschule Geisenheim University, 65366 Geisenheim, GermanyDepartment of Crop Protection, Hochschule Geisenheim University, 65366 Geisenheim, GermanyJulius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Biological Control, 69221 Dossenheim, GermanyAlthough synthetic pesticides play a major role in plant protection, their application needs to be reduced because of their negative impact on the environment. This applies also to copper preparations, which are used in organic farming. For this reason, alternatives with less impact on the environment are urgently needed. In this context, we evaluated eight isolates of the genus <i>Lysobacter</i> (mainly <i>Lysobacter enzymogenes</i>) for their activity against plant pathogens. In vitro, the investigated <i>Lysobacter</i> isolates showed broad antagonistic activity against several phytopathogenic fungi, oomycetes and bacteria. Enzyme assays revealed diverse activities for the tested isolates. The most promising <i>L. enzymogenes</i> isolate (LEC) was used for further detailed analyses of its efficacy and effective working concentrations. The experiments included in vitro spore and sporangia germination tests and leaf disc assays as well as ad planta growth chamber trials against <i>Alternaria solani</i> and <i>Phytophthora infestans</i> on tomato plants, <i>Pseudoperonospora cubensis</i> on cucumbers and <i>Venturia inaequalis</i> on young potted apple trees. When applied on leaves, dilutions of a culture suspension of LEC had a concentration-dependent, protective effect against the tested pathogens. In all pathosystems tested, the effective concentrations were in the range of 2.5–5% and similarly efficacious to common plant protection agents containing copper hydroxide, wettable sulphur or fenhexamid. Thus, the isolate of <i>L. enzymogenes</i> identified in this study exhibits a broad activity against common plant pathogens and is therefore a promising candidate for the development of a microbial biocontrol agent.https://www.mdpi.com/2223-7747/12/3/682<i>Lysobacter</i>biocontrolantagonismBCAbiological fungicideplant protection |
| spellingShingle | Christian Drenker Doris El Mazouar Gerrit Bücker Sonja Weißhaupt Eveline Wienke Eckhard Koch Stefan Kunz Annette Reineke Yvonne Rondot Ada Linkies Characterization of a Disease-Suppressive Isolate of <i>Lysobacter enzymogenes</i> with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops Plants <i>Lysobacter</i> biocontrol antagonism BCA biological fungicide plant protection |
| title | Characterization of a Disease-Suppressive Isolate of <i>Lysobacter enzymogenes</i> with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops |
| title_full | Characterization of a Disease-Suppressive Isolate of <i>Lysobacter enzymogenes</i> with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops |
| title_fullStr | Characterization of a Disease-Suppressive Isolate of <i>Lysobacter enzymogenes</i> with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops |
| title_full_unstemmed | Characterization of a Disease-Suppressive Isolate of <i>Lysobacter enzymogenes</i> with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops |
| title_short | Characterization of a Disease-Suppressive Isolate of <i>Lysobacter enzymogenes</i> with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops |
| title_sort | characterization of a disease suppressive isolate of i lysobacter enzymogenes i with broad antagonistic activity against bacterial oomycetal and fungal pathogens in different crops |
| topic | <i>Lysobacter</i> biocontrol antagonism BCA biological fungicide plant protection |
| url | https://www.mdpi.com/2223-7747/12/3/682 |
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