In Vitro Fertilization of Immature Oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients
Background: Men with azoospermia require testicular and epididymal sperm aspiration for intracytoplasmic sperm injection. Women over 37 years of age demonstrate an increase number of immature oocytes after induction of ovulation. The development of a technique for in vitro maturation (IVM) and ferti...
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| Format: | Article |
| Language: | English |
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College of Medicine University of Baghdad
2008-01-01
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| Series: | مجلة كلية الطب |
| Subjects: | |
| Online Access: | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1329 |
| _version_ | 1797332855496376320 |
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| author | Saeeda A. M. Alanssari Jie. Liu, Mike Kjelland M. Ridha- Albananchi Duane C. Kraemer |
| author_facet | Saeeda A. M. Alanssari Jie. Liu, Mike Kjelland M. Ridha- Albananchi Duane C. Kraemer |
| author_sort | Saeeda A. M. Alanssari |
| collection | DOAJ |
| description | Background: Men with azoospermia require testicular and epididymal sperm aspiration for intracytoplasmic sperm injection. Women over 37 years of age demonstrate
an increase number of immature oocytes after induction of ovulation. The development of a technique for in vitro maturation (IVM) and fertilization (IVF) of
the oocytes using testicular, epididymal and vassal sperm (TS, ES, VS, respectively) will be of therapeutic value for the treatment of azoospermic patients.
Objectives: The goal of the study was to develop an animal model for the treatment of infertile couples with obstructive azoospermia and immature oocytes.
Materials and Methods: Canine ovaries and testes were collected from a local animal clinic. Immature oocytes were retrieved from the ovaries and cultured in modified tissue
culture medium (MTCM). The mature oocytes were in vitro fertilized by TS, ES, and VS. The normality of the fertilized oocytes was studied.
Results: Sperm motility index was significantly higher (P<0.01) in sperm retrieved from the vas deference compared to ES and TS. The concentration of the sperm was
significantly higher (P<0.05) in the testes compared to epididymis and vas deference while VS and ES normal morphology were significantly (p<0.01) better than TS. Viable oocyte
percentage was significantly higher (P<O.05) in 72 hours versus 96 hours culture durations. Significant higher IVF was reported in VS compared to other groups (P < 0.05) and the SPI
was significantly higher after 96 versus 72 hours duration (P<O.05).
Conclusion: IVM of the oocytes in MTCM for 72 and 96 h resulted in a significant increase in IVM rate compared to 48,120, and 144 h durations. In vitro viable mature
oocytes showed higher IVF rates when fertilized with VS compared to ES and TS. The results showed that canine oocytes are good model for azoospermic patients. |
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| institution | Directory Open Access Journal |
| issn | 0041-9419 2410-8057 |
| language | English |
| last_indexed | 2024-03-08T07:54:24Z |
| publishDate | 2008-01-01 |
| publisher | College of Medicine University of Baghdad |
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| series | مجلة كلية الطب |
| spelling | doaj.art-0fceea9ac2bb413e98c282d1a4784b5c2024-02-02T14:08:01ZengCollege of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572008-01-01494In Vitro Fertilization of Immature Oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile PatientsSaeeda A. M. Alanssari0Jie. Liu, Mike Kjelland1M. Ridha- Albananchi2Duane C. Kraemer3Reproductive Sciences Laboratory, Department of Physiology and Pharmacology, Texas A&M University, College Station, Texas, USA and *Department of Physiology, College of Medicine, University of Baghdad, Baghdad, Iraq.Reproductive Sciences Laboratory, Department of Physiology and Pharmacology, Texas A&M University, College Station, Texas, USA and *Department of Physiology, College of Medicine, University of Baghdad, Baghdad, Iraq.Reproductive Sciences Laboratory, Department of Physiology and Pharmacology, Texas A&M University, College Station, Texas, USA and *Department of Physiology, College of Medicine, University of Baghdad, Baghdad, Iraq.Reproductive Sciences Laboratory, Department of Physiology and Pharmacology, Texas A&M University, College Station, Texas, USA and *Department of Physiology, College of Medicine, University of Baghdad, Baghdad, Iraq.Background: Men with azoospermia require testicular and epididymal sperm aspiration for intracytoplasmic sperm injection. Women over 37 years of age demonstrate an increase number of immature oocytes after induction of ovulation. The development of a technique for in vitro maturation (IVM) and fertilization (IVF) of the oocytes using testicular, epididymal and vassal sperm (TS, ES, VS, respectively) will be of therapeutic value for the treatment of azoospermic patients. Objectives: The goal of the study was to develop an animal model for the treatment of infertile couples with obstructive azoospermia and immature oocytes. Materials and Methods: Canine ovaries and testes were collected from a local animal clinic. Immature oocytes were retrieved from the ovaries and cultured in modified tissue culture medium (MTCM). The mature oocytes were in vitro fertilized by TS, ES, and VS. The normality of the fertilized oocytes was studied. Results: Sperm motility index was significantly higher (P<0.01) in sperm retrieved from the vas deference compared to ES and TS. The concentration of the sperm was significantly higher (P<0.05) in the testes compared to epididymis and vas deference while VS and ES normal morphology were significantly (p<0.01) better than TS. Viable oocyte percentage was significantly higher (P<O.05) in 72 hours versus 96 hours culture durations. Significant higher IVF was reported in VS compared to other groups (P < 0.05) and the SPI was significantly higher after 96 versus 72 hours duration (P<O.05). Conclusion: IVM of the oocytes in MTCM for 72 and 96 h resulted in a significant increase in IVM rate compared to 48,120, and 144 h durations. In vitro viable mature oocytes showed higher IVF rates when fertilized with VS compared to ES and TS. The results showed that canine oocytes are good model for azoospermic patients.http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1329IVM, IVF, Testicular Sperm, Animal model, Azoospermia |
| spellingShingle | Saeeda A. M. Alanssari Jie. Liu, Mike Kjelland M. Ridha- Albananchi Duane C. Kraemer In Vitro Fertilization of Immature Oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients مجلة كلية الطب IVM, IVF, Testicular Sperm, Animal model, Azoospermia |
| title | In Vitro Fertilization of Immature Oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients |
| title_full | In Vitro Fertilization of Immature Oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients |
| title_fullStr | In Vitro Fertilization of Immature Oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients |
| title_full_unstemmed | In Vitro Fertilization of Immature Oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients |
| title_short | In Vitro Fertilization of Immature Oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients |
| title_sort | in vitro fertilization of immature oocytes by testicular sperm animal model for azoospermic infertile patients |
| topic | IVM, IVF, Testicular Sperm, Animal model, Azoospermia |
| url | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1329 |
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