Synthesis, Characterization, and Antimicrobial and Antiproliferative Effects of CuO-TiO<sub>2</sub>-Chitosan-Escin Nanocomposites on Human Leukemic MOLT4 Cells
Nanocomposites comprised of CuO-TiO<sub>2</sub>-chitosan-escin, which has adjustable physicochemical properties, provide a solution for therapeutic selectivity in cancer treatment. By controlling the intrinsic signaling primarily through the mitochondrial signaling pathway, we desired na...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/21/3753 |
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| author | Abozer Y. Elderdery Abdulaziz H. Alhamidi Ahmed M. E. Elkhalifa Maryam M. Althobiti Nawal Eltayeb Omer Mahdi H. Alsugoor Naif Alsuhaymi Entesar M. Atebien Siddiqa M. A. Hamza Badr Alzahrani Fehaid Alanazi Suresh S. Kumar Pooi Ling Mok |
| author_facet | Abozer Y. Elderdery Abdulaziz H. Alhamidi Ahmed M. E. Elkhalifa Maryam M. Althobiti Nawal Eltayeb Omer Mahdi H. Alsugoor Naif Alsuhaymi Entesar M. Atebien Siddiqa M. A. Hamza Badr Alzahrani Fehaid Alanazi Suresh S. Kumar Pooi Ling Mok |
| author_sort | Abozer Y. Elderdery |
| collection | DOAJ |
| description | Nanocomposites comprised of CuO-TiO<sub>2</sub>-chitosan-escin, which has adjustable physicochemical properties, provide a solution for therapeutic selectivity in cancer treatment. By controlling the intrinsic signaling primarily through the mitochondrial signaling pathway, we desired nanocomposites with enhanced anticancer activity by containing CuO-TiO<sub>2</sub>-chitosan-escin. The metal oxides CuO and TiO<sub>2</sub>, the natural polymer chitosan, and a phytochemical compound escin were combined to form CuO-TiO<sub>2</sub>-chitosan-escin nanocomposites. The synthesized nanocomposites were confirmed and characterized using FTIR spectroscopy, TEM, and UV-Vis absorption spectroscopy. A human leukemia cell line (MOLT-4) was used to assess the efficacy and selectivity of nanocomposites. Based on a cytotoxicity study, CuO-TiO<sub>2</sub>-chitosan-escin nanocomposites had inhibition concentrations (IC<sub>50</sub>) of 13.68, 8.9, and 7.14 µg/mL against human T lymphoblast cells after 24, 48, and 72 h of incubation, respectively. Compared with untreated MOLT-4 cells, CuO-TiO<sub>2</sub>-chitosan-escin nanocomposite-treated cells significantly increased (<i>p</i> < 0.05) caspase-3, -8, and -9 and decreased the levels of antioxidant enzymes GR, SOD, and GSH. Furthermore, MDA for lipid peroxidase and ROS levels significantly increased (<i>p</i> < 0.05) in the treated cells than in the untreated cells. Remarkably, CuO-TiO<sub>2</sub>-chitosan-escin nanocomposite-mediated control of cell cycles were mainly achieved through the activation of caspase-3, -8, and -9. |
| first_indexed | 2024-03-09T18:46:39Z |
| format | Article |
| id | doaj.art-0fd2a12c17d04187b748bee542f686ff |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T18:46:39Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-0fd2a12c17d04187b748bee542f686ff2023-11-24T06:08:46ZengMDPI AGNanomaterials2079-49912022-10-011221375310.3390/nano12213753Synthesis, Characterization, and Antimicrobial and Antiproliferative Effects of CuO-TiO<sub>2</sub>-Chitosan-Escin Nanocomposites on Human Leukemic MOLT4 CellsAbozer Y. Elderdery0Abdulaziz H. Alhamidi1Ahmed M. E. Elkhalifa2Maryam M. Althobiti3Nawal Eltayeb Omer4Mahdi H. Alsugoor5Naif Alsuhaymi6Entesar M. Atebien7Siddiqa M. A. Hamza8Badr Alzahrani9Fehaid Alanazi10Suresh S. Kumar11Pooi Ling Mok12Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 42421, Saudi ArabiaClinical Laboratory Sciences Department, College of Applied Medical Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Public Health, College of Health Sciences, Saudi Electronic University, Riyadh 13316, Saudi ArabiaDepartment of Clinical Laboratory Science, College of Applied Medical Science, King Saud University, Shaqra 15572, Saudi ArabiaHereditary Blood Disease Center, Al Ehsaa, Saudi ArabiaDepartment of Emergency Medical Services, Faculty of Health Sciences, AlQunfudah, Umm Al-Qura University, Makkah 21912, Saudi ArabiaDepartment of Emergency Medical Services, Faculty of Health Sciences, AlQunfudah, Umm Al-Qura University, Makkah 21912, Saudi ArabiaDepartment of Clinical Laboratory Science, College of Applied Medical Science, King Saud University, Shaqra 15572, Saudi ArabiaCollege of Medicine, Department of Pathology, Umm Alqura University Algunfuda, Mecca 24382, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 42421, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences-AlQurayyat, Jouf University, Sakaka 42421, Saudi ArabiaCentre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Chennai 600073, IndiaDepartment of Biomedical Science, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaNanocomposites comprised of CuO-TiO<sub>2</sub>-chitosan-escin, which has adjustable physicochemical properties, provide a solution for therapeutic selectivity in cancer treatment. By controlling the intrinsic signaling primarily through the mitochondrial signaling pathway, we desired nanocomposites with enhanced anticancer activity by containing CuO-TiO<sub>2</sub>-chitosan-escin. The metal oxides CuO and TiO<sub>2</sub>, the natural polymer chitosan, and a phytochemical compound escin were combined to form CuO-TiO<sub>2</sub>-chitosan-escin nanocomposites. The synthesized nanocomposites were confirmed and characterized using FTIR spectroscopy, TEM, and UV-Vis absorption spectroscopy. A human leukemia cell line (MOLT-4) was used to assess the efficacy and selectivity of nanocomposites. Based on a cytotoxicity study, CuO-TiO<sub>2</sub>-chitosan-escin nanocomposites had inhibition concentrations (IC<sub>50</sub>) of 13.68, 8.9, and 7.14 µg/mL against human T lymphoblast cells after 24, 48, and 72 h of incubation, respectively. Compared with untreated MOLT-4 cells, CuO-TiO<sub>2</sub>-chitosan-escin nanocomposite-treated cells significantly increased (<i>p</i> < 0.05) caspase-3, -8, and -9 and decreased the levels of antioxidant enzymes GR, SOD, and GSH. Furthermore, MDA for lipid peroxidase and ROS levels significantly increased (<i>p</i> < 0.05) in the treated cells than in the untreated cells. Remarkably, CuO-TiO<sub>2</sub>-chitosan-escin nanocomposite-mediated control of cell cycles were mainly achieved through the activation of caspase-3, -8, and -9.https://www.mdpi.com/2079-4991/12/21/3753CuO-TiO<sub>2</sub>-chitosan-escin nanocompositesantioxidantreactive oxygen speciesanticancercaspase |
| spellingShingle | Abozer Y. Elderdery Abdulaziz H. Alhamidi Ahmed M. E. Elkhalifa Maryam M. Althobiti Nawal Eltayeb Omer Mahdi H. Alsugoor Naif Alsuhaymi Entesar M. Atebien Siddiqa M. A. Hamza Badr Alzahrani Fehaid Alanazi Suresh S. Kumar Pooi Ling Mok Synthesis, Characterization, and Antimicrobial and Antiproliferative Effects of CuO-TiO<sub>2</sub>-Chitosan-Escin Nanocomposites on Human Leukemic MOLT4 Cells Nanomaterials CuO-TiO<sub>2</sub>-chitosan-escin nanocomposites antioxidant reactive oxygen species anticancer caspase |
| title | Synthesis, Characterization, and Antimicrobial and Antiproliferative Effects of CuO-TiO<sub>2</sub>-Chitosan-Escin Nanocomposites on Human Leukemic MOLT4 Cells |
| title_full | Synthesis, Characterization, and Antimicrobial and Antiproliferative Effects of CuO-TiO<sub>2</sub>-Chitosan-Escin Nanocomposites on Human Leukemic MOLT4 Cells |
| title_fullStr | Synthesis, Characterization, and Antimicrobial and Antiproliferative Effects of CuO-TiO<sub>2</sub>-Chitosan-Escin Nanocomposites on Human Leukemic MOLT4 Cells |
| title_full_unstemmed | Synthesis, Characterization, and Antimicrobial and Antiproliferative Effects of CuO-TiO<sub>2</sub>-Chitosan-Escin Nanocomposites on Human Leukemic MOLT4 Cells |
| title_short | Synthesis, Characterization, and Antimicrobial and Antiproliferative Effects of CuO-TiO<sub>2</sub>-Chitosan-Escin Nanocomposites on Human Leukemic MOLT4 Cells |
| title_sort | synthesis characterization and antimicrobial and antiproliferative effects of cuo tio sub 2 sub chitosan escin nanocomposites on human leukemic molt4 cells |
| topic | CuO-TiO<sub>2</sub>-chitosan-escin nanocomposites antioxidant reactive oxygen species anticancer caspase |
| url | https://www.mdpi.com/2079-4991/12/21/3753 |
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