Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020
Avian reovirus (ARV) is the primary pathogen responsible for viral arthritis. In this study, 2340 samples with suspected viral arthritis were collected from 2019 to 2020 in 16 provinces of China to investigate the prevalence of ARV in China and to characterize the molecular genetic evolution of epid...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/2/485 |
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| author | Dong Liu Zhong Zou Shanshan Song Hongxiang Liu Xiao Gong Bin Li Ping Liu Qunyi Wang Fengbo Liu Dongzu Luan Xiang Zhang Yuanzhao Du Meilin Jin |
| author_facet | Dong Liu Zhong Zou Shanshan Song Hongxiang Liu Xiao Gong Bin Li Ping Liu Qunyi Wang Fengbo Liu Dongzu Luan Xiang Zhang Yuanzhao Du Meilin Jin |
| author_sort | Dong Liu |
| collection | DOAJ |
| description | Avian reovirus (ARV) is the primary pathogen responsible for viral arthritis. In this study, 2340 samples with suspected viral arthritis were collected from 2019 to 2020 in 16 provinces of China to investigate the prevalence of ARV in China and to characterize the molecular genetic evolution of epidemic strains. From 113 samples analyzed by RT-PCR, 46 strains of avian reovirus were successfully isolated and identified. The genetic evolution of the σC gene showed that 46 strains were distributed in 1–5 branches, with the largest number of strains in branches 1 and 2. The σC gene homology among the strains was low, with approximately 62% homology in branches 4 and 5 and about 55% in the remaining branches. The strains circulating during the ARV epidemic in different provinces were distributed in different branches. The SPF chickens were immunized with inactivated vaccines containing strains from branches 1 and 4 to analyze the cross-immune protection elicited by different branches of ARV strains. A challenge protection test was performed using strains in branches 1, 2, 4, and 5. Our results showed that inactivated vaccines containing strains from branches 1 and 4 could fully protect from strains in branches 1, 4, and 5. The results of this study revealed the genetic diversity among the endemic strains of ARV in China from 2019 to 2020. Each genotype strain elicited partial cross-protection, providing a scientific basis for the prevention and control of ARV. |
| first_indexed | 2024-03-11T08:01:21Z |
| format | Article |
| id | doaj.art-0fd71fd4509d486babd532bbded9a227 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T08:01:21Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-0fd71fd4509d486babd532bbded9a2272023-11-16T23:45:04ZengMDPI AGVaccines2076-393X2023-02-0111248510.3390/vaccines11020485Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020Dong Liu0Zhong Zou1Shanshan Song2Hongxiang Liu3Xiao Gong4Bin Li5Ping Liu6Qunyi Wang7Fengbo Liu8Dongzu Luan9Xiang Zhang10Yuanzhao Du11Meilin Jin12State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaYEBIO Bio-Engineering Co., Ltd. of Qingdao, Qingdao 266032, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaAvian reovirus (ARV) is the primary pathogen responsible for viral arthritis. In this study, 2340 samples with suspected viral arthritis were collected from 2019 to 2020 in 16 provinces of China to investigate the prevalence of ARV in China and to characterize the molecular genetic evolution of epidemic strains. From 113 samples analyzed by RT-PCR, 46 strains of avian reovirus were successfully isolated and identified. The genetic evolution of the σC gene showed that 46 strains were distributed in 1–5 branches, with the largest number of strains in branches 1 and 2. The σC gene homology among the strains was low, with approximately 62% homology in branches 4 and 5 and about 55% in the remaining branches. The strains circulating during the ARV epidemic in different provinces were distributed in different branches. The SPF chickens were immunized with inactivated vaccines containing strains from branches 1 and 4 to analyze the cross-immune protection elicited by different branches of ARV strains. A challenge protection test was performed using strains in branches 1, 2, 4, and 5. Our results showed that inactivated vaccines containing strains from branches 1 and 4 could fully protect from strains in branches 1, 4, and 5. The results of this study revealed the genetic diversity among the endemic strains of ARV in China from 2019 to 2020. Each genotype strain elicited partial cross-protection, providing a scientific basis for the prevention and control of ARV.https://www.mdpi.com/2076-393X/11/2/485avian reovirus virusgenetic evolutionchallenge protection |
| spellingShingle | Dong Liu Zhong Zou Shanshan Song Hongxiang Liu Xiao Gong Bin Li Ping Liu Qunyi Wang Fengbo Liu Dongzu Luan Xiang Zhang Yuanzhao Du Meilin Jin Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020 Vaccines avian reovirus virus genetic evolution challenge protection |
| title | Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020 |
| title_full | Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020 |
| title_fullStr | Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020 |
| title_full_unstemmed | Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020 |
| title_short | Epidemiological Analysis of Avian Reovirus in China and Research on the Immune Protection of Different Genotype Strains from 2019 to 2020 |
| title_sort | epidemiological analysis of avian reovirus in china and research on the immune protection of different genotype strains from 2019 to 2020 |
| topic | avian reovirus virus genetic evolution challenge protection |
| url | https://www.mdpi.com/2076-393X/11/2/485 |
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