Association of Omentin rs2274907 and FTO rs9939609 gene polymorphisms with insulin resistance in Iranian individuals with newly diagnosed type 2 diabetes
Abstract Background Insulin resistance (IR) and fat accumulation in visceral adipose tissue are key players in developing type 2 diabetes (T2D). Several adipose tissue derived-gene polymorphisms are related to higher body mass index (BMI), insulin resistance and T2D. The association of omentin rs227...
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-019-1085-5 |
| _version_ | 1828485762631335936 |
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| author | Amirhosein Khoshi Mehdi Kaffash Bajestani Habibesadat Shakeri Golnaz Goodarzi Fatemeh Azizi |
| author_facet | Amirhosein Khoshi Mehdi Kaffash Bajestani Habibesadat Shakeri Golnaz Goodarzi Fatemeh Azizi |
| author_sort | Amirhosein Khoshi |
| collection | DOAJ |
| description | Abstract Background Insulin resistance (IR) and fat accumulation in visceral adipose tissue are key players in developing type 2 diabetes (T2D). Several adipose tissue derived-gene polymorphisms are related to higher body mass index (BMI), insulin resistance and T2D. The association of omentin rs2274907 (Val109Asp) and fat-mass and obesity-associated (FTO) rs9939609 gene polymorphisms with overweight/obesity and T2D is controversial. The aim of this study was to determine the association between omentin Val109Asp and FTO rs9939609 polymorphisms and insulin resistance in newly-diagnosed T2D patients. Methods The case-control study included 83 newly-diagnosed T2D patients and 85 healthy matched controls, aged 20–80 years. Fasting blood glucose and insulin levels were measured by the enzymatic method and enzyme-linked-immunosorbent assay, respectively. Insulin resistance was calculated using the homeostasis model assessment (HOMA) index. Genotyping was examined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results There are significant differences between both omentin Val109Asp and FTO rs9939609 polymorphisms and studied individuals (P = 0.011 and P = 0.0001, respectively). Both genetic polymorphisms of omentin Val109Asp and FTO rs9939609 (T/A) are significantly related to higher HOMA index (P = 0.030 and P = 0.046, respectively). However, omentin Val109Asp polymorphism was only related to individuals who were overweight/obese. Additionally, both omentin Val109Asp and FTO rs9939609 polymorphisms were significantly positively correlated to familial history of diabetes (P = 0.046 and P = 0.024, respectively). Conclusions Omentin V109D and FTO rs9939609 genetic variations may change insulin metabolism and have key roles in developing T2D through insulin resistance. Thus, the evaluation of these polymorphic regions may be helpful for predicting type 2 diabetes. |
| first_indexed | 2024-12-11T09:21:05Z |
| format | Article |
| id | doaj.art-0fd7d8da02bf4ca99a09dd3c9cc5fc79 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-11T09:21:05Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-0fd7d8da02bf4ca99a09dd3c9cc5fc792022-12-22T01:13:16ZengBMCLipids in Health and Disease1476-511X2019-06-0118111010.1186/s12944-019-1085-5Association of Omentin rs2274907 and FTO rs9939609 gene polymorphisms with insulin resistance in Iranian individuals with newly diagnosed type 2 diabetesAmirhosein Khoshi0Mehdi Kaffash Bajestani1Habibesadat Shakeri2Golnaz Goodarzi3Fatemeh Azizi4Department of Clinical Biochemistry, School of Medicine, North Khorasan University of Medical SciencesEducational Development Center, North Khorasan University of Medical SciencesClinical Endocrinology, Department of Endocrinology and Internal Medicine, Imam Hassan Hospital, North Khorasan University of Medical SciencesDepartment of Clinical Biochemistry, School of Medicine, North Khorasan University of Medical SciencesStudent Research Committee, North Khorasan University of Medical SciencesAbstract Background Insulin resistance (IR) and fat accumulation in visceral adipose tissue are key players in developing type 2 diabetes (T2D). Several adipose tissue derived-gene polymorphisms are related to higher body mass index (BMI), insulin resistance and T2D. The association of omentin rs2274907 (Val109Asp) and fat-mass and obesity-associated (FTO) rs9939609 gene polymorphisms with overweight/obesity and T2D is controversial. The aim of this study was to determine the association between omentin Val109Asp and FTO rs9939609 polymorphisms and insulin resistance in newly-diagnosed T2D patients. Methods The case-control study included 83 newly-diagnosed T2D patients and 85 healthy matched controls, aged 20–80 years. Fasting blood glucose and insulin levels were measured by the enzymatic method and enzyme-linked-immunosorbent assay, respectively. Insulin resistance was calculated using the homeostasis model assessment (HOMA) index. Genotyping was examined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results There are significant differences between both omentin Val109Asp and FTO rs9939609 polymorphisms and studied individuals (P = 0.011 and P = 0.0001, respectively). Both genetic polymorphisms of omentin Val109Asp and FTO rs9939609 (T/A) are significantly related to higher HOMA index (P = 0.030 and P = 0.046, respectively). However, omentin Val109Asp polymorphism was only related to individuals who were overweight/obese. Additionally, both omentin Val109Asp and FTO rs9939609 polymorphisms were significantly positively correlated to familial history of diabetes (P = 0.046 and P = 0.024, respectively). Conclusions Omentin V109D and FTO rs9939609 genetic variations may change insulin metabolism and have key roles in developing T2D through insulin resistance. Thus, the evaluation of these polymorphic regions may be helpful for predicting type 2 diabetes.http://link.springer.com/article/10.1186/s12944-019-1085-5Type 2 diabetesInsulin resistanceOmentinFat mass-and obesity associated (FTO)Gene polymorphism |
| spellingShingle | Amirhosein Khoshi Mehdi Kaffash Bajestani Habibesadat Shakeri Golnaz Goodarzi Fatemeh Azizi Association of Omentin rs2274907 and FTO rs9939609 gene polymorphisms with insulin resistance in Iranian individuals with newly diagnosed type 2 diabetes Lipids in Health and Disease Type 2 diabetes Insulin resistance Omentin Fat mass-and obesity associated (FTO) Gene polymorphism |
| title | Association of Omentin rs2274907 and FTO rs9939609 gene polymorphisms with insulin resistance in Iranian individuals with newly diagnosed type 2 diabetes |
| title_full | Association of Omentin rs2274907 and FTO rs9939609 gene polymorphisms with insulin resistance in Iranian individuals with newly diagnosed type 2 diabetes |
| title_fullStr | Association of Omentin rs2274907 and FTO rs9939609 gene polymorphisms with insulin resistance in Iranian individuals with newly diagnosed type 2 diabetes |
| title_full_unstemmed | Association of Omentin rs2274907 and FTO rs9939609 gene polymorphisms with insulin resistance in Iranian individuals with newly diagnosed type 2 diabetes |
| title_short | Association of Omentin rs2274907 and FTO rs9939609 gene polymorphisms with insulin resistance in Iranian individuals with newly diagnosed type 2 diabetes |
| title_sort | association of omentin rs2274907 and fto rs9939609 gene polymorphisms with insulin resistance in iranian individuals with newly diagnosed type 2 diabetes |
| topic | Type 2 diabetes Insulin resistance Omentin Fat mass-and obesity associated (FTO) Gene polymorphism |
| url | http://link.springer.com/article/10.1186/s12944-019-1085-5 |
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