Analysis of association of -174G/C interleukin 6 polymorphism and therapeutic response to methotrexate in rheumatoid arthritis
Introduction: Rheumatoid arthritis (RA) is a systemic autoimmune disease with IL-6 as a main mediator of systemic and localized inflammation. The most often used drug for the treatment of RA is methotrexate (MTX). Aim: To explore if there is an association between the 174G/C polymorphism in IL6 gene...
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| Format: | Article |
| Language: | English |
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University of Belgrade, Medical Faculty
2018-01-01
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| Series: | Medicinski Podmladak |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2018/0369-15271804074J.pdf |
| _version_ | 1818256804400857088 |
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| author | Joksimović Anastasija Khouri Christina Jekić Biljana |
| author_facet | Joksimović Anastasija Khouri Christina Jekić Biljana |
| author_sort | Joksimović Anastasija |
| collection | DOAJ |
| description | Introduction: Rheumatoid arthritis (RA) is a systemic autoimmune disease with IL-6 as a main mediator of systemic and localized inflammation. The most often used drug for the treatment of RA is methotrexate (MTX). Aim: To explore if there is an association between the 174G/C polymorphism in IL6 gene and response to methotrexate therapy in RA patients. Materials and Methods: Study has included 131 RA patients treated and followed at the Institute of Rheumatology in Belgrade. Clinical response to the MTX therapy was conducted following the EULAR response criteria. Each patient's DNA was isolated by salting-out method. For each patient IL6 174G/C polymorphism genotype was determined by allele specific PCR method. For statistical analyses we have used SPSS program version 17.0. Results: According to EULAR response criteria 103 (78.6 %) patients were responders 12(9.2 %) good and 91 (69.5 %) moderate response) and 28 (21.4 %) were non responders. Among all patients in 47 (35.9 %) we have detected GG genotype, in 69 (52.7 %) GC genotype and in 15 (11.5 %) CC genotype. We have observed no association between genotypes or alleles and response to MTX therapy. Conclusion: No association of -174G/C IL-6 polymorphism and therapeutic response to MTX in RA patients in Serbian population was observed. |
| first_indexed | 2024-12-12T17:33:34Z |
| format | Article |
| id | doaj.art-0fd821f523f84bc1a8c01a88b28a0f62 |
| institution | Directory Open Access Journal |
| issn | 0369-1527 2466-5525 |
| language | English |
| last_indexed | 2024-12-12T17:33:34Z |
| publishDate | 2018-01-01 |
| publisher | University of Belgrade, Medical Faculty |
| record_format | Article |
| series | Medicinski Podmladak |
| spelling | doaj.art-0fd821f523f84bc1a8c01a88b28a0f622022-12-22T00:17:18ZengUniversity of Belgrade, Medical FacultyMedicinski Podmladak0369-15272466-55252018-01-0169474780369-15271804074JAnalysis of association of -174G/C interleukin 6 polymorphism and therapeutic response to methotrexate in rheumatoid arthritisJoksimović Anastasija0Khouri Christina1Jekić Biljana2Univerzitet u Beogradu, Medicinski fakultet, BeogradUniverzitet u Beogradu, Medicinski fakultet, BeogradUniverzitet u Beogradu, Medicinski fakultet, Institut za humanu genetiku, BeogradIntroduction: Rheumatoid arthritis (RA) is a systemic autoimmune disease with IL-6 as a main mediator of systemic and localized inflammation. The most often used drug for the treatment of RA is methotrexate (MTX). Aim: To explore if there is an association between the 174G/C polymorphism in IL6 gene and response to methotrexate therapy in RA patients. Materials and Methods: Study has included 131 RA patients treated and followed at the Institute of Rheumatology in Belgrade. Clinical response to the MTX therapy was conducted following the EULAR response criteria. Each patient's DNA was isolated by salting-out method. For each patient IL6 174G/C polymorphism genotype was determined by allele specific PCR method. For statistical analyses we have used SPSS program version 17.0. Results: According to EULAR response criteria 103 (78.6 %) patients were responders 12(9.2 %) good and 91 (69.5 %) moderate response) and 28 (21.4 %) were non responders. Among all patients in 47 (35.9 %) we have detected GG genotype, in 69 (52.7 %) GC genotype and in 15 (11.5 %) CC genotype. We have observed no association between genotypes or alleles and response to MTX therapy. Conclusion: No association of -174G/C IL-6 polymorphism and therapeutic response to MTX in RA patients in Serbian population was observed.https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2018/0369-15271804074J.pdfIL-6PolymorphismRheumatoid ArthritisMethotrexate |
| spellingShingle | Joksimović Anastasija Khouri Christina Jekić Biljana Analysis of association of -174G/C interleukin 6 polymorphism and therapeutic response to methotrexate in rheumatoid arthritis Medicinski Podmladak IL-6 Polymorphism Rheumatoid Arthritis Methotrexate |
| title | Analysis of association of -174G/C interleukin 6 polymorphism and therapeutic response to methotrexate in rheumatoid arthritis |
| title_full | Analysis of association of -174G/C interleukin 6 polymorphism and therapeutic response to methotrexate in rheumatoid arthritis |
| title_fullStr | Analysis of association of -174G/C interleukin 6 polymorphism and therapeutic response to methotrexate in rheumatoid arthritis |
| title_full_unstemmed | Analysis of association of -174G/C interleukin 6 polymorphism and therapeutic response to methotrexate in rheumatoid arthritis |
| title_short | Analysis of association of -174G/C interleukin 6 polymorphism and therapeutic response to methotrexate in rheumatoid arthritis |
| title_sort | analysis of association of 174g c interleukin 6 polymorphism and therapeutic response to methotrexate in rheumatoid arthritis |
| topic | IL-6 Polymorphism Rheumatoid Arthritis Methotrexate |
| url | https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2018/0369-15271804074J.pdf |
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