Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcoding
The meiofauna is an important part of the marine ecosystem, but its composition and distribution patterns are relatively unexplored. Here we assessed the biodiversity and community structure of meiofauna from five locations on the Swedish western and southern coasts using a high-throughput DNA seque...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2020-04-01
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| Series: | Biodiversity Data Journal |
| Subjects: | |
| Online Access: | https://bdj.pensoft.net/article/51813/download/pdf/ |
| _version_ | 1818765491185909760 |
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| author | Sarah Atherton Ulf Jondelius |
| author_facet | Sarah Atherton Ulf Jondelius |
| author_sort | Sarah Atherton |
| collection | DOAJ |
| description | The meiofauna is an important part of the marine ecosystem, but its composition and distribution patterns are relatively unexplored. Here we assessed the biodiversity and community structure of meiofauna from five locations on the Swedish western and southern coasts using a high-throughput DNA sequencing (metabarcoding) approach. The mitochondrial cytochrome oxidase 1 (COI) mini-barcode and nuclear 18S small ribosomal subunit (18S) V1-V2 region were amplified and sequenced using Illumina MiSeq technology. Our analyses revealed a higher number of species than previously found in other areas: thirteen samples comprising 6.5 dm3 sediment revealed 708 COI and 1,639 18S metazoan OTUs. Across all sites, the majority of the metazoan biodiversity was assigned to Arthropoda, Nematoda and Platyhelminthes. Alpha and beta diversity measurements showed that community composition differed significantly amongst sites. OTUs initially assigned to Acoela, Gastrotricha and the two Platyhelminthes sub-groups Macrostomorpha and Rhabdocoela were further investigated and assigned to species using a phylogeny-based taxonomy approach. Our results demonstrate that there is great potential for discovery of new meiofauna species even in some of the most extensively studied locations. |
| first_indexed | 2024-12-18T08:18:56Z |
| format | Article |
| id | doaj.art-0fda9a8ea2c74a6ebb3c2c00fb7adbf8 |
| institution | Directory Open Access Journal |
| issn | 1314-2828 |
| language | English |
| last_indexed | 2024-12-18T08:18:56Z |
| publishDate | 2020-04-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Biodiversity Data Journal |
| spelling | doaj.art-0fda9a8ea2c74a6ebb3c2c00fb7adbf82022-12-21T21:14:46ZengPensoft PublishersBiodiversity Data Journal1314-28282020-04-01814010.3897/BDJ.8.e5181351813Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcodingSarah Atherton0Ulf Jondelius1Swedish Museum of Natural HistorySwedish Museum of Natural HistoryThe meiofauna is an important part of the marine ecosystem, but its composition and distribution patterns are relatively unexplored. Here we assessed the biodiversity and community structure of meiofauna from five locations on the Swedish western and southern coasts using a high-throughput DNA sequencing (metabarcoding) approach. The mitochondrial cytochrome oxidase 1 (COI) mini-barcode and nuclear 18S small ribosomal subunit (18S) V1-V2 region were amplified and sequenced using Illumina MiSeq technology. Our analyses revealed a higher number of species than previously found in other areas: thirteen samples comprising 6.5 dm3 sediment revealed 708 COI and 1,639 18S metazoan OTUs. Across all sites, the majority of the metazoan biodiversity was assigned to Arthropoda, Nematoda and Platyhelminthes. Alpha and beta diversity measurements showed that community composition differed significantly amongst sites. OTUs initially assigned to Acoela, Gastrotricha and the two Platyhelminthes sub-groups Macrostomorpha and Rhabdocoela were further investigated and assigned to species using a phylogeny-based taxonomy approach. Our results demonstrate that there is great potential for discovery of new meiofauna species even in some of the most extensively studied locations.https://bdj.pensoft.net/article/51813/download/pdf/Illumina Mi-SeqHigh Throughput SequencingAcoel |
| spellingShingle | Sarah Atherton Ulf Jondelius Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcoding Biodiversity Data Journal Illumina Mi-Seq High Throughput Sequencing Acoel |
| title | Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcoding |
| title_full | Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcoding |
| title_fullStr | Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcoding |
| title_full_unstemmed | Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcoding |
| title_short | Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcoding |
| title_sort | biodiversity between sand grains meiofauna composition across southern and western sweden assessed by metabarcoding |
| topic | Illumina Mi-Seq High Throughput Sequencing Acoel |
| url | https://bdj.pensoft.net/article/51813/download/pdf/ |
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