From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research
Ion channels play a crucial role in diverse physiological processes, including neurotransmission and muscle contraction. Venomous creatures exploit the vital function of ion channels by producing toxins in their venoms that specifically target these ion channels to facilitate prey capture upon a bit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1249336/full |
| _version_ | 1797737463710482432 |
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| author | Shirin Ahmadi Melisa Benard-Valle Kim Boddum Fernanda C. Cardoso Fernanda C. Cardoso Glenn F. King Glenn F. King Andreas Hougaard Laustsen Anne Ljungars |
| author_facet | Shirin Ahmadi Melisa Benard-Valle Kim Boddum Fernanda C. Cardoso Fernanda C. Cardoso Glenn F. King Glenn F. King Andreas Hougaard Laustsen Anne Ljungars |
| author_sort | Shirin Ahmadi |
| collection | DOAJ |
| description | Ion channels play a crucial role in diverse physiological processes, including neurotransmission and muscle contraction. Venomous creatures exploit the vital function of ion channels by producing toxins in their venoms that specifically target these ion channels to facilitate prey capture upon a bite or a sting. Envenoming can therefore lead to ion channel dysregulation, which for humans can result in severe medical complications that often necessitate interventions such as antivenom administration. Conversely, the discovery of highly potent and selective venom toxins with the capability of distinguishing between different isoforms and subtypes of ion channels has led to the development of beneficial therapeutics that are now in the clinic. This review encompasses the historical evolution of electrophysiology methodologies, highlighting their contributions to venom and antivenom research, including venom-based drug discovery and evaluation of antivenom efficacy. By discussing the applications and advancements in patch-clamp techniques, this review underscores the profound impact of electrophysiology in unravelling the intricate interplay between ion channels and venom toxins, ultimately leading to the development of drugs for envenoming and ion channel-related pathologies. |
| first_indexed | 2024-03-12T13:29:06Z |
| format | Article |
| id | doaj.art-0fdee07fa94244deb089186a6d8f2735 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-12T13:29:06Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-0fdee07fa94244deb089186a6d8f27352023-08-24T16:31:05ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-08-011410.3389/fphar.2023.12493361249336From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom researchShirin Ahmadi0Melisa Benard-Valle1Kim Boddum2Fernanda C. Cardoso3Fernanda C. Cardoso4Glenn F. King5Glenn F. King6Andreas Hougaard Laustsen7Anne Ljungars8Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkSophion Bioscience, Ballerup, DenmarkInstitute for Molecular Bioscience, University of Queensland, St Lucia, QLD, AustraliaAustralian Research Council Centre of Excellence for Innovations in Protein and Peptide Science, University of Queensland, St Lucia, QLD, AustraliaInstitute for Molecular Bioscience, University of Queensland, St Lucia, QLD, AustraliaAustralian Research Council Centre of Excellence for Innovations in Protein and Peptide Science, University of Queensland, St Lucia, QLD, AustraliaDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkIon channels play a crucial role in diverse physiological processes, including neurotransmission and muscle contraction. Venomous creatures exploit the vital function of ion channels by producing toxins in their venoms that specifically target these ion channels to facilitate prey capture upon a bite or a sting. Envenoming can therefore lead to ion channel dysregulation, which for humans can result in severe medical complications that often necessitate interventions such as antivenom administration. Conversely, the discovery of highly potent and selective venom toxins with the capability of distinguishing between different isoforms and subtypes of ion channels has led to the development of beneficial therapeutics that are now in the clinic. This review encompasses the historical evolution of electrophysiology methodologies, highlighting their contributions to venom and antivenom research, including venom-based drug discovery and evaluation of antivenom efficacy. By discussing the applications and advancements in patch-clamp techniques, this review underscores the profound impact of electrophysiology in unravelling the intricate interplay between ion channels and venom toxins, ultimately leading to the development of drugs for envenoming and ion channel-related pathologies.https://www.frontiersin.org/articles/10.3389/fphar.2023.1249336/fullelectrophysiologypatch-clampion channelvenomantivenomdrug discovery |
| spellingShingle | Shirin Ahmadi Melisa Benard-Valle Kim Boddum Fernanda C. Cardoso Fernanda C. Cardoso Glenn F. King Glenn F. King Andreas Hougaard Laustsen Anne Ljungars From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research Frontiers in Pharmacology electrophysiology patch-clamp ion channel venom antivenom drug discovery |
| title | From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research |
| title_full | From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research |
| title_fullStr | From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research |
| title_full_unstemmed | From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research |
| title_short | From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research |
| title_sort | from squid giant axon to automated patch clamp electrophysiology in venom and antivenom research |
| topic | electrophysiology patch-clamp ion channel venom antivenom drug discovery |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1249336/full |
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