<em>luxS</em> Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?
AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the <em>luxS</em>-mutant strain. But the AI-2 production proce...
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MDPI AG
2012-05-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/12/5/6176 |
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| author | Zhengwei Huang Zheng Liu Jingping Liang Zisheng Tang Rui Ma Qian Wang Zhiyan He Yuejian Hu Yuntao Jiang |
| author_facet | Zhengwei Huang Zheng Liu Jingping Liang Zisheng Tang Rui Ma Qian Wang Zhiyan He Yuejian Hu Yuntao Jiang |
| author_sort | Zhengwei Huang |
| collection | DOAJ |
| description | AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the <em>luxS</em>-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by<em> luxS</em>-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system—not the metabolic aspect—is responsible for such a regulatory function. In this study, we constructed plasmids infused with<em> sahH</em> and induced protein expression in the <em>luxS</em>-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for <em>luxS</em> mutant regulation. |
| first_indexed | 2024-04-11T18:04:57Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T18:04:57Z |
| publishDate | 2012-05-01 |
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| spelling | doaj.art-0fdf5b687e2a4921aed44863fcdb0e562022-12-22T04:10:22ZengMDPI AGSensors1424-82202012-05-011256176618510.3390/s120506176<em>luxS</em> Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?Zhengwei HuangZheng LiuJingping LiangZisheng TangRui MaQian WangZhiyan HeYuejian HuYuntao JiangAI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the <em>luxS</em>-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by<em> luxS</em>-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system—not the metabolic aspect—is responsible for such a regulatory function. In this study, we constructed plasmids infused with<em> sahH</em> and induced protein expression in the <em>luxS</em>-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for <em>luxS</em> mutant regulation.http://www.mdpi.com/1424-8220/12/5/6176quorum sensingAI-2<em>luxS</em><em> sahH</em> |
| spellingShingle | Zhengwei Huang Zheng Liu Jingping Liang Zisheng Tang Rui Ma Qian Wang Zhiyan He Yuejian Hu Yuntao Jiang <em>luxS</em> Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? Sensors quorum sensing AI-2 <em>luxS</em> <em> sahH</em> |
| title | <em>luxS</em> Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
| title_full | <em>luxS</em> Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
| title_fullStr | <em>luxS</em> Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
| title_full_unstemmed | <em>luxS</em> Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
| title_short | <em>luxS</em> Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? |
| title_sort | lt em gt luxs lt em gt mutant regulation quorum sensing impairment or methylation disorder |
| topic | quorum sensing AI-2 <em>luxS</em> <em> sahH</em> |
| url | http://www.mdpi.com/1424-8220/12/5/6176 |
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