Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma
Objective:The aim of this study was to evaluate bcl-2, bax, and c-erbB-2 expressions in primary and secondary acquired cholesteatoma and to indicate the role of apoptosis and accompanying increased cellular proliferation in the pathogenesis of cholesteatoma.Methods:Samples obtained from the skin of...
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| Format: | Article |
| Language: | English |
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Galenos Yayincilik
2015-06-01
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| Series: | Turkish Archives of Otorhinolaryngology |
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http://turkarchotolaryngol.net/archives/archive-detail/article-preview/evaluation-of-bcl-2-bax-and-c-erbb-2-levels-in-chr/43336
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| _version_ | 1797911357990895616 |
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| author | Özgür Işık Turgut Karlıdağ Bengü Çobanoğlu Şimşek Erol Keleş İrfan Kaygusuz Şinasi Yalçın İsrafil Orhan Emrah Sapmaz |
| author_facet | Özgür Işık Turgut Karlıdağ Bengü Çobanoğlu Şimşek Erol Keleş İrfan Kaygusuz Şinasi Yalçın İsrafil Orhan Emrah Sapmaz |
| author_sort | Özgür Işık |
| collection | DOAJ |
| description | Objective:The aim of this study was to evaluate bcl-2, bax, and c-erbB-2 expressions in primary and secondary acquired cholesteatoma and to indicate the role of apoptosis and accompanying increased cellular proliferation in the pathogenesis of cholesteatoma.Methods:Samples obtained from the skin of the external ear canal (EEC) of patients operated for chronic otitis media (COM) without cholesteatoma constituted Group 1; samples from the EEC skin of patients in Group 3 operated for COM with cholesteatoma and from the EEC skin of patients in Group 4 constituted Group 2; samples obtained from the cholesteatoma matrix of patients operated for COM with primary acquired cholesteatoma constituted Group 3; and samples obtained from the cholesteatoma matrix of patients operated for COM with secondary acquired cholesteatoma constituted Group 4. The assessment of the positive cell ratio was based on the presence of the following findings and was semiquantitatively classified into four groups: 0, no staining; + cell staining (weak positive staining: 1%–33%); ++ cell staining (moderately positive staining: 34%–66%); and +++ cell staining (strong positive staining: 67%–100%).Results:Comparison of the staining scores of bcl-2, bax, and c-erbB-2 revealed a statistically insignificant difference in the staining of samples obtained from the EEC skin (p>0.05). Decreased bcl-2 expression and increased bax and c-erbB-2 expressions were determined in primary and secondary acquired cholesteatoma epithelium compared with the EEC skin of patients operated for COM with or without cholesteatoma, and the differences were found to be statistically significant (p<0.05).Conclusion:In acquired cholesteatoma epithelium, the finding of decreased bcl-2 expression as well as increased bax and c-erbB-2 expressions compared with the EEC skin is an indicator of the increase in both cellular proliferation and apoptosis. |
| first_indexed | 2024-04-10T11:39:19Z |
| format | Article |
| id | doaj.art-0fdf922cdbdf453e86bf64059dbe8058 |
| institution | Directory Open Access Journal |
| issn | 2667-7474 |
| language | English |
| last_indexed | 2024-04-10T11:39:19Z |
| publishDate | 2015-06-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Turkish Archives of Otorhinolaryngology |
| spelling | doaj.art-0fdf922cdbdf453e86bf64059dbe80582023-02-15T16:17:35ZengGalenos YayincilikTurkish Archives of Otorhinolaryngology2667-74742015-06-01532556110.5152/tao.2015.115913049054Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without CholesteatomaÖzgür Işık0Turgut Karlıdağ1Bengü Çobanoğlu Şimşek2Erol Keleş3İrfan Kaygusuz4Şinasi Yalçın5İsrafil Orhan6Emrah Sapmaz7 Department of Otorhinolaryngology, Fırat University Faculty of Medicine, Elazığ, Turkey Department of Otorhinolaryngology, Fırat University Faculty of Medicine, Elazığ, Turkey Department of Pathology, Fırat University Faculty of Medicine, Elazığ, Turkey Department of Otorhinolaryngology, Fırat University Faculty of Medicine, Elazığ, Turkey Department of Otorhinolaryngology, Fırat University Faculty of Medicine, Elazığ, Turkey Department of Otorhinolaryngology, Fırat University Faculty of Medicine, Elazığ, Turkey Department of Otorhinolaryngology, Kahramanmaraş Sütçü İmam Faculty of Medicine, Kahramanmaraş, Turkey Department of Otorhinolaryngology, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey Objective:The aim of this study was to evaluate bcl-2, bax, and c-erbB-2 expressions in primary and secondary acquired cholesteatoma and to indicate the role of apoptosis and accompanying increased cellular proliferation in the pathogenesis of cholesteatoma.Methods:Samples obtained from the skin of the external ear canal (EEC) of patients operated for chronic otitis media (COM) without cholesteatoma constituted Group 1; samples from the EEC skin of patients in Group 3 operated for COM with cholesteatoma and from the EEC skin of patients in Group 4 constituted Group 2; samples obtained from the cholesteatoma matrix of patients operated for COM with primary acquired cholesteatoma constituted Group 3; and samples obtained from the cholesteatoma matrix of patients operated for COM with secondary acquired cholesteatoma constituted Group 4. The assessment of the positive cell ratio was based on the presence of the following findings and was semiquantitatively classified into four groups: 0, no staining; + cell staining (weak positive staining: 1%–33%); ++ cell staining (moderately positive staining: 34%–66%); and +++ cell staining (strong positive staining: 67%–100%).Results:Comparison of the staining scores of bcl-2, bax, and c-erbB-2 revealed a statistically insignificant difference in the staining of samples obtained from the EEC skin (p>0.05). Decreased bcl-2 expression and increased bax and c-erbB-2 expressions were determined in primary and secondary acquired cholesteatoma epithelium compared with the EEC skin of patients operated for COM with or without cholesteatoma, and the differences were found to be statistically significant (p<0.05).Conclusion:In acquired cholesteatoma epithelium, the finding of decreased bcl-2 expression as well as increased bax and c-erbB-2 expressions compared with the EEC skin is an indicator of the increase in both cellular proliferation and apoptosis. http://turkarchotolaryngol.net/archives/archive-detail/article-preview/evaluation-of-bcl-2-bax-and-c-erbb-2-levels-in-chr/43336 cholesteatomaapoptosiscell proliferationbcl-2baxc-erbb-2 |
| spellingShingle | Özgür Işık Turgut Karlıdağ Bengü Çobanoğlu Şimşek Erol Keleş İrfan Kaygusuz Şinasi Yalçın İsrafil Orhan Emrah Sapmaz Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma Turkish Archives of Otorhinolaryngology cholesteatoma apoptosis cell proliferation bcl-2 bax c-erbb-2 |
| title | Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma |
| title_full | Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma |
| title_fullStr | Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma |
| title_full_unstemmed | Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma |
| title_short | Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma |
| title_sort | evaluation of bcl 2 bax and c erbb 2 levels in chronic otitis patients with or without cholesteatoma |
| topic | cholesteatoma apoptosis cell proliferation bcl-2 bax c-erbb-2 |
| url |
http://turkarchotolaryngol.net/archives/archive-detail/article-preview/evaluation-of-bcl-2-bax-and-c-erbb-2-levels-in-chr/43336
|
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