Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid
In this study, to establish whether serum amyloid A (SAA) 3 plays a role in the defense against bacterial infection in mouse mammary epithelium, normal murine mammary gland (NMuMG) epithelial cells were stimulated with lipopolysaccharide (LPS) and lipoteichoic acid (LTA). LPS and LTA significantly e...
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MDPI AG
2021-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1548 |
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| author | Sato Kamiya Kaori Shimizu Ayaka Okada Yasuo Inoshima |
| author_facet | Sato Kamiya Kaori Shimizu Ayaka Okada Yasuo Inoshima |
| author_sort | Sato Kamiya |
| collection | DOAJ |
| description | In this study, to establish whether serum amyloid A (SAA) 3 plays a role in the defense against bacterial infection in mouse mammary epithelium, normal murine mammary gland (NMuMG) epithelial cells were stimulated with lipopolysaccharide (LPS) and lipoteichoic acid (LTA). LPS and LTA significantly enhanced mRNA expression level of the <i>Saa3</i> gene, whereas no significant change was observed in the <i>Saa1</i> mRNA level. Furthermore, LPS induced SAA3 protein expression more strongly than LTA, whereas neither LPS nor LTA significantly affected SAA1 protein expression. These data indicate that the expression of SAA3 in mouse mammary epithelial cells was increased by the stimulation with bacterial antigens. SAA3 has been reported to stimulate neutrophils in the intestinal epithelium and increase interleukin-22 expression, which induces activation of the innate immune system and production of antibacterial proteins, such as antimicrobial peptides. Therefore, collectively, these data suggest that SAA3 is involved in the defense against bacterial infection in mouse mammary epithelium. |
| first_indexed | 2024-03-10T11:03:05Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T11:03:05Z |
| publishDate | 2021-05-01 |
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| series | Animals |
| spelling | doaj.art-0fe0d188ffb3421d8424cbf71debe31b2023-11-21T21:18:26ZengMDPI AGAnimals2076-26152021-05-01116154810.3390/ani11061548Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic AcidSato Kamiya0Kaori Shimizu1Ayaka Okada2Yasuo Inoshima3Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, JapanLaboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, JapanLaboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, JapanLaboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, JapanIn this study, to establish whether serum amyloid A (SAA) 3 plays a role in the defense against bacterial infection in mouse mammary epithelium, normal murine mammary gland (NMuMG) epithelial cells were stimulated with lipopolysaccharide (LPS) and lipoteichoic acid (LTA). LPS and LTA significantly enhanced mRNA expression level of the <i>Saa3</i> gene, whereas no significant change was observed in the <i>Saa1</i> mRNA level. Furthermore, LPS induced SAA3 protein expression more strongly than LTA, whereas neither LPS nor LTA significantly affected SAA1 protein expression. These data indicate that the expression of SAA3 in mouse mammary epithelial cells was increased by the stimulation with bacterial antigens. SAA3 has been reported to stimulate neutrophils in the intestinal epithelium and increase interleukin-22 expression, which induces activation of the innate immune system and production of antibacterial proteins, such as antimicrobial peptides. Therefore, collectively, these data suggest that SAA3 is involved in the defense against bacterial infection in mouse mammary epithelium.https://www.mdpi.com/2076-2615/11/6/1548lipopolysaccharidelipoteichoic acidmouse mammary epithelial cellsserum amyloid A1serum amyloid A3 |
| spellingShingle | Sato Kamiya Kaori Shimizu Ayaka Okada Yasuo Inoshima Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid Animals lipopolysaccharide lipoteichoic acid mouse mammary epithelial cells serum amyloid A1 serum amyloid A3 |
| title | Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid |
| title_full | Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid |
| title_fullStr | Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid |
| title_full_unstemmed | Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid |
| title_short | Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid |
| title_sort | induction of serum amyloid a3 in mouse mammary epithelial cells stimulated with lipopolysaccharide and lipoteichoic acid |
| topic | lipopolysaccharide lipoteichoic acid mouse mammary epithelial cells serum amyloid A1 serum amyloid A3 |
| url | https://www.mdpi.com/2076-2615/11/6/1548 |
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