Recombinant production of human Aquaporin-1 to an exceptional high membrane density in Saccharomyces cerevisiae.

Recombinant production of human Aquaporin-1 to an exceptional high membrane density in Saccharomyces cerevisiae.

In the present paper we explored the capacity of yeast Saccharomyces cerevisiae as host for heterologous expression of human Aquaporin-1. Aquaporin-1 cDNA was expressed from a galactose inducible promoter situated on a plasmid with an adjustable copy number. Human Aquaporin-1 was C-terminally tagged...

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Bibliographic Details
Main Authors: Julie Bomholt, Claus Hélix-Nielsen, Peter Scharff-Poulsen, Per Amstrup Pedersen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3569440?pdf=render

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