LPCAT1 overexpression promotes the progression of hepatocellular carcinoma
Abstract Background Hepatocellular carcinoma (HCC) remains one of the most common malignant neoplasms. Lysophosphatidylcholine acyltransferase 1 (LPCAT1) plays a key role in the lipid remodelling and is correlated with various neoplasms. Nonetheless, the biological functions and molecular mechanisms...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | Cancer Cell International |
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| Online Access: | https://doi.org/10.1186/s12935-021-02130-4 |
| _version_ | 1819124788984020992 |
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| author | Rong-Quan He Jian-Di Li Xiu-Fang Du Yi-Wu Dang Lin-Jie Yang Zhi-Guang Huang Li-Min Liu Liu-Feng Liao Hong Yang Gang Chen |
| author_facet | Rong-Quan He Jian-Di Li Xiu-Fang Du Yi-Wu Dang Lin-Jie Yang Zhi-Guang Huang Li-Min Liu Liu-Feng Liao Hong Yang Gang Chen |
| author_sort | Rong-Quan He |
| collection | DOAJ |
| description | Abstract Background Hepatocellular carcinoma (HCC) remains one of the most common malignant neoplasms. Lysophosphatidylcholine acyltransferase 1 (LPCAT1) plays a key role in the lipid remodelling and is correlated with various neoplasms. Nonetheless, the biological functions and molecular mechanisms of LPCAT1 underlying HCC remain obscure. Methods In the present study, we investigated the role of LPCAT1 in the progression of HCC. In-house RT-qPCR, tissue microarrays, and immunohistochemistry were performed to detect the expression levels and the clinical value of LPCAT1 in HCC. External datasets were downloaded to confirm the results. Proliferation, migration, invasiveness, cell cycle, and apoptosis assays were conducted to reveal the biological effects LPCAT1 has on SMMC-7721 and Huh7 cells. HCC differentially expressed genes and LPCAT1 co-expressed genes were identified to explore the molecular mechanisms underlying HCC progression. Results LPCAT1 showed upregulated expression in 3715 HCC specimens as opposed to 3105 non-tumour specimens. Additionally, LPCAT1 might be an independent prognostic factor for HCC. LPCAT1-knockout hampered cellular proliferation, migration, and metastasis in SMMC-7721 and Huh7 cells. More importantly, the cell cycle and chemical carcinogenesis were the two most enriched signalling pathways. Conclusions The present study demonstrated that increased LPCAT1 correlated with poor prognosis in HCC patients and fuelled HCC progression by promoting cellular growth, migration, and metastasis. |
| first_indexed | 2024-12-22T07:29:49Z |
| format | Article |
| id | doaj.art-0fe3d6f393394ab3a2d3ae5b3b19b196 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-22T07:29:49Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-0fe3d6f393394ab3a2d3ae5b3b19b1962022-12-21T18:34:03ZengBMCCancer Cell International1475-28672021-08-0121111710.1186/s12935-021-02130-4LPCAT1 overexpression promotes the progression of hepatocellular carcinomaRong-Quan He0Jian-Di Li1Xiu-Fang Du2Yi-Wu Dang3Lin-Jie Yang4Zhi-Guang Huang5Li-Min Liu6Liu-Feng Liao7Hong Yang8Gang Chen9Department of Oncology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Toxicology, College of Pharmacy, Guangxi Medical UniversityDepartment of Pharmacy, Guangxi Medical University Cancer HospitalThe Ultrasonics Division of Radiology Department, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityAbstract Background Hepatocellular carcinoma (HCC) remains one of the most common malignant neoplasms. Lysophosphatidylcholine acyltransferase 1 (LPCAT1) plays a key role in the lipid remodelling and is correlated with various neoplasms. Nonetheless, the biological functions and molecular mechanisms of LPCAT1 underlying HCC remain obscure. Methods In the present study, we investigated the role of LPCAT1 in the progression of HCC. In-house RT-qPCR, tissue microarrays, and immunohistochemistry were performed to detect the expression levels and the clinical value of LPCAT1 in HCC. External datasets were downloaded to confirm the results. Proliferation, migration, invasiveness, cell cycle, and apoptosis assays were conducted to reveal the biological effects LPCAT1 has on SMMC-7721 and Huh7 cells. HCC differentially expressed genes and LPCAT1 co-expressed genes were identified to explore the molecular mechanisms underlying HCC progression. Results LPCAT1 showed upregulated expression in 3715 HCC specimens as opposed to 3105 non-tumour specimens. Additionally, LPCAT1 might be an independent prognostic factor for HCC. LPCAT1-knockout hampered cellular proliferation, migration, and metastasis in SMMC-7721 and Huh7 cells. More importantly, the cell cycle and chemical carcinogenesis were the two most enriched signalling pathways. Conclusions The present study demonstrated that increased LPCAT1 correlated with poor prognosis in HCC patients and fuelled HCC progression by promoting cellular growth, migration, and metastasis.https://doi.org/10.1186/s12935-021-02130-4Hepatocellular carcinomaLysophosphatidylcholine acyltransferase 1Functional experimentMolecular mechanismClinical applications |
| spellingShingle | Rong-Quan He Jian-Di Li Xiu-Fang Du Yi-Wu Dang Lin-Jie Yang Zhi-Guang Huang Li-Min Liu Liu-Feng Liao Hong Yang Gang Chen LPCAT1 overexpression promotes the progression of hepatocellular carcinoma Cancer Cell International Hepatocellular carcinoma Lysophosphatidylcholine acyltransferase 1 Functional experiment Molecular mechanism Clinical applications |
| title | LPCAT1 overexpression promotes the progression of hepatocellular carcinoma |
| title_full | LPCAT1 overexpression promotes the progression of hepatocellular carcinoma |
| title_fullStr | LPCAT1 overexpression promotes the progression of hepatocellular carcinoma |
| title_full_unstemmed | LPCAT1 overexpression promotes the progression of hepatocellular carcinoma |
| title_short | LPCAT1 overexpression promotes the progression of hepatocellular carcinoma |
| title_sort | lpcat1 overexpression promotes the progression of hepatocellular carcinoma |
| topic | Hepatocellular carcinoma Lysophosphatidylcholine acyltransferase 1 Functional experiment Molecular mechanism Clinical applications |
| url | https://doi.org/10.1186/s12935-021-02130-4 |
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