Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS)
<p>Abstract</p> <p>Background</p> <p>The liver is the largest human internal organ – it is composed of multiple cell types and plays a vital role in fulfilling the body's metabolic needs and maintaining homeostasis. Of these cell types the hepatocytes, which accoun...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-07-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/8/207 |
| _version_ | 1811282676298022912 |
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| author | Li Yi-Xue Zhang Xue-Gong Li Ting-Ting Wang Yun Wang Xiao-Wo Hong Yi Deng Qing Deng Fu-Xing Zhang Yun-Li Hao Pei Huang Jian Yang Peng-Yuan Wang Hong-Yang Han Ze-Guang |
| author_facet | Li Yi-Xue Zhang Xue-Gong Li Ting-Ting Wang Yun Wang Xiao-Wo Hong Yi Deng Qing Deng Fu-Xing Zhang Yun-Li Hao Pei Huang Jian Yang Peng-Yuan Wang Hong-Yang Han Ze-Guang |
| author_sort | Li Yi-Xue |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The liver is the largest human internal organ – it is composed of multiple cell types and plays a vital role in fulfilling the body's metabolic needs and maintaining homeostasis. Of these cell types the hepatocytes, which account for three-quarters of the liver's volume, perform its main functions. To discover the molecular basis of hepatocyte function, we employed Massively Parallel Signature Sequencing (MPSS) to determine the transcriptomic profile of adult human hepatocytes obtained by laser capture microdissection (LCM).</p> <p>Results</p> <p>10,279 UniGene clusters, representing 7,475 known genes, were detected in human hepatocytes. In addition, 1,819 unique MPSS signatures matching the antisense strand of 1,605 non-redundant UniGene clusters (such as <it>APOC1</it>, <it>APOC2</it>, <it>APOB </it>and <it>APOH</it>) were highly expressed in hepatocytes.</p> <p>Conclusion</p> <p>Apart from a large number of protein-coding genes, some of the antisense transcripts expressed in hepatocytes could play important roles in transcriptional interference via a <it>cis</it>-/<it>trans</it>-regulation mechanism. Our result provided a comprehensively transcriptomic atlas of human hepatocytes using MPSS technique, which could be served as an available resource for an in-depth understanding of human liver biology and diseases.</p> |
| first_indexed | 2024-04-13T01:56:50Z |
| format | Article |
| id | doaj.art-0fe92437c9fd4956b0622eca0cac0517 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T01:56:50Z |
| publishDate | 2007-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-0fe92437c9fd4956b0622eca0cac05172022-12-22T03:07:44ZengBMCBMC Genomics1471-21642007-07-018120710.1186/1471-2164-8-207Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS)Li Yi-XueZhang Xue-GongLi Ting-TingWang YunWang Xiao-WoHong YiDeng QingDeng Fu-XingZhang Yun-LiHao PeiHuang JianYang Peng-YuanWang Hong-YangHan Ze-Guang<p>Abstract</p> <p>Background</p> <p>The liver is the largest human internal organ – it is composed of multiple cell types and plays a vital role in fulfilling the body's metabolic needs and maintaining homeostasis. Of these cell types the hepatocytes, which account for three-quarters of the liver's volume, perform its main functions. To discover the molecular basis of hepatocyte function, we employed Massively Parallel Signature Sequencing (MPSS) to determine the transcriptomic profile of adult human hepatocytes obtained by laser capture microdissection (LCM).</p> <p>Results</p> <p>10,279 UniGene clusters, representing 7,475 known genes, were detected in human hepatocytes. In addition, 1,819 unique MPSS signatures matching the antisense strand of 1,605 non-redundant UniGene clusters (such as <it>APOC1</it>, <it>APOC2</it>, <it>APOB </it>and <it>APOH</it>) were highly expressed in hepatocytes.</p> <p>Conclusion</p> <p>Apart from a large number of protein-coding genes, some of the antisense transcripts expressed in hepatocytes could play important roles in transcriptional interference via a <it>cis</it>-/<it>trans</it>-regulation mechanism. Our result provided a comprehensively transcriptomic atlas of human hepatocytes using MPSS technique, which could be served as an available resource for an in-depth understanding of human liver biology and diseases.</p>http://www.biomedcentral.com/1471-2164/8/207 |
| spellingShingle | Li Yi-Xue Zhang Xue-Gong Li Ting-Ting Wang Yun Wang Xiao-Wo Hong Yi Deng Qing Deng Fu-Xing Zhang Yun-Li Hao Pei Huang Jian Yang Peng-Yuan Wang Hong-Yang Han Ze-Guang Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS) BMC Genomics |
| title | Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS) |
| title_full | Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS) |
| title_fullStr | Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS) |
| title_full_unstemmed | Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS) |
| title_short | Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS) |
| title_sort | discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing mpss |
| url | http://www.biomedcentral.com/1471-2164/8/207 |
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