Effects of gradual differences in trypsin inhibitor activity on the estimation of digestible amino acids in soybean expellers for broiler chickens
ABSTRACT: The present study investigated the effect of varying trypsin inhibitor activity (TIA) in differently processed soybean expellers on apparent prececal amino acid (AA) digestibility in male broiler chickens. Two different raw soybean batches were treated using varying processing techniques a...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122000451 |
| _version_ | 1811260591718793216 |
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| author | S. Kuenz S. Thurner D. Hoffmann K. Kraft M. Wiltafsky-Martin K. Damme W. Windisch D. Brugger |
| author_facet | S. Kuenz S. Thurner D. Hoffmann K. Kraft M. Wiltafsky-Martin K. Damme W. Windisch D. Brugger |
| author_sort | S. Kuenz |
| collection | DOAJ |
| description | ABSTRACT: The present study investigated the effect of varying trypsin inhibitor activity (TIA) in differently processed soybean expellers on apparent prececal amino acid (AA) digestibility in male broiler chickens. Two different raw soybean batches were treated using varying processing techniques and intensities. In this way, 45 expeller extracted soybean meal (ESBM) variants were created. The processed soybean variants were then merged into a basal diet (160 g/kg crude protein [CP]) at 2 inclusion levels (15%, 30%) resulting in 90 different diets plus one basal diet (0.4 mg/g–8.5 mg/g TIA). All diets contained 0.5% titanium dioxide. A total of 5,460-day-old male broilers (Ross 308) were allocated on d 14 to 546 pens (10 birds/pen) after a starter phase (CP 215 g/kg, 14 g/kg Lysine, 12.5 MJ ME/kg). The 91 experimental diets were fed ad libitum until d 22. Subsequently, birds were euthanized and digesta of the terminal ileum was collected for determination of AA digestibility. TIA depressed the prececal digestibility of every single AA significantly in a straight linear fashion (P < 0.001). Sulfur-containing AA expressed the strongest suppression by TIA with cystine showing the lowest apparent prececal digestibility measured (10.6% at 23.6 mg/g TIA in raw ESBM). The present data demonstrate that TIA severely depresses digestibility of essential and nonessential AA in a straight linear fashion. On the one hand, this questions the usefulness of defined upper limits of TIA in soy products whereas on the other hand, TIA must be considered when testing raw components for their feed protein value in vivo. |
| first_indexed | 2024-04-12T18:49:47Z |
| format | Article |
| id | doaj.art-0fe9a355709a404a9b6663e984ff237f |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-12T18:49:47Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-0fe9a355709a404a9b6663e984ff237f2022-12-22T03:20:31ZengElsevierPoultry Science0032-57912022-04-011014101740Effects of gradual differences in trypsin inhibitor activity on the estimation of digestible amino acids in soybean expellers for broiler chickensS. Kuenz0S. Thurner1D. Hoffmann2K. Kraft3M. Wiltafsky-Martin4K. Damme5W. Windisch6D. Brugger7Technical University of Munich (TUM), TUM School of Life Sciences, Chair of Animal Nutrition, 85354 Freising, GermanyBavarian State Research Center for Agriculture, Institute for Agricultural Engineering and Animal Husbandry, 85354 Freising, GermanyTechnical University of Munich (TUM), TUM School of Life Sciences, Chair of Animal Nutrition, 85354 Freising, Germany; Bavarian State Research Center for Agriculture, Institute for Agricultural Engineering and Animal Husbandry, 85354 Freising, GermanyBavarian State Research Center for Agriculture, Institute for Agricultural Engineering and Animal Husbandry, 85354 Freising, Germany; Bavarian State Research Center for Agriculture, Department for Education and Poultry Research, 97318 Kitzingen, GermanyEvonik Operations GmbH, 63457 Hanau-Wolfgang, GermanyBavarian State Research Center for Agriculture, Department for Education and Poultry Research, 97318 Kitzingen, GermanyTechnical University of Munich (TUM), TUM School of Life Sciences, Chair of Animal Nutrition, 85354 Freising, GermanyTechnical University of Munich (TUM), TUM School of Life Sciences, Chair of Animal Nutrition, 85354 Freising, Germany; University of Zurich, Vetsuisse-Faculty, Institute of Animal Nutrition and Dietetics, 8057 Zurich, Switzerland; Corresponding author:ABSTRACT: The present study investigated the effect of varying trypsin inhibitor activity (TIA) in differently processed soybean expellers on apparent prececal amino acid (AA) digestibility in male broiler chickens. Two different raw soybean batches were treated using varying processing techniques and intensities. In this way, 45 expeller extracted soybean meal (ESBM) variants were created. The processed soybean variants were then merged into a basal diet (160 g/kg crude protein [CP]) at 2 inclusion levels (15%, 30%) resulting in 90 different diets plus one basal diet (0.4 mg/g–8.5 mg/g TIA). All diets contained 0.5% titanium dioxide. A total of 5,460-day-old male broilers (Ross 308) were allocated on d 14 to 546 pens (10 birds/pen) after a starter phase (CP 215 g/kg, 14 g/kg Lysine, 12.5 MJ ME/kg). The 91 experimental diets were fed ad libitum until d 22. Subsequently, birds were euthanized and digesta of the terminal ileum was collected for determination of AA digestibility. TIA depressed the prececal digestibility of every single AA significantly in a straight linear fashion (P < 0.001). Sulfur-containing AA expressed the strongest suppression by TIA with cystine showing the lowest apparent prececal digestibility measured (10.6% at 23.6 mg/g TIA in raw ESBM). The present data demonstrate that TIA severely depresses digestibility of essential and nonessential AA in a straight linear fashion. On the one hand, this questions the usefulness of defined upper limits of TIA in soy products whereas on the other hand, TIA must be considered when testing raw components for their feed protein value in vivo.http://www.sciencedirect.com/science/article/pii/S0032579122000451Amino acidbroilerdigestibilitysoybeantrypsin inhibitor |
| spellingShingle | S. Kuenz S. Thurner D. Hoffmann K. Kraft M. Wiltafsky-Martin K. Damme W. Windisch D. Brugger Effects of gradual differences in trypsin inhibitor activity on the estimation of digestible amino acids in soybean expellers for broiler chickens Poultry Science Amino acid broiler digestibility soybean trypsin inhibitor |
| title | Effects of gradual differences in trypsin inhibitor activity on the estimation of digestible amino acids in soybean expellers for broiler chickens |
| title_full | Effects of gradual differences in trypsin inhibitor activity on the estimation of digestible amino acids in soybean expellers for broiler chickens |
| title_fullStr | Effects of gradual differences in trypsin inhibitor activity on the estimation of digestible amino acids in soybean expellers for broiler chickens |
| title_full_unstemmed | Effects of gradual differences in trypsin inhibitor activity on the estimation of digestible amino acids in soybean expellers for broiler chickens |
| title_short | Effects of gradual differences in trypsin inhibitor activity on the estimation of digestible amino acids in soybean expellers for broiler chickens |
| title_sort | effects of gradual differences in trypsin inhibitor activity on the estimation of digestible amino acids in soybean expellers for broiler chickens |
| topic | Amino acid broiler digestibility soybean trypsin inhibitor |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122000451 |
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