Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice
Citrus juices are a rich source of bioactive compounds with various and well-known health benefits. The aim of this study was to investigate the polyphenols and ascorbic acid content as well as to investigate the antioxidant, anti-inflammatory and anti-angiogenic properties of the juice of an ancien...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2020.593506/full |
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| author | Antonella Smeriglio Marcella Denaro Valeria D’Angelo Maria Paola Germanò Domenico Trombetta |
| author_facet | Antonella Smeriglio Marcella Denaro Valeria D’Angelo Maria Paola Germanò Domenico Trombetta |
| author_sort | Antonella Smeriglio |
| collection | DOAJ |
| description | Citrus juices are a rich source of bioactive compounds with various and well-known health benefits. The aim of this study was to investigate the polyphenols and ascorbic acid content as well as to investigate the antioxidant, anti-inflammatory and anti-angiogenic properties of the juice of an ancient Mediterranean species, Citrus lumia Risso (CLJ). The antioxidant and anti-inflammatory activities were evaluated by several in vitro cell-free and cell-based assays, whereas two different in vivo models, the chick chorioallantoic membrane (CAM) and the zebrafish embryos, were used to characterize the anti-angiogenic properties. Twenty-eight polyphenols were identified by RP-LC-DAD-ESI-MS analysis (flavonoids 68.82% and phenolic acids 31.18%) with 1-caffeoyl-5-feruloylquinic acid and kaempferol 3′-rhamnoside, which represent the most abundant compounds (25.70 and 23.12%, respectively). HPLC-DAD analysis showed a high ascorbic acid content (352 mg/kg of CLJ), which contributes with polyphenols to the marked and dose-dependent antioxidant and anti-inflammatory properties observed. CLJ showed strong and dose-dependent anti-angiogenic activity as highlighted by the inhibition of blood vessel formation on CAMs and the decrease of endogenous alkaline phosphatase on zebrafish embryos. Moreover, within the concentration range tested, no dead or malformed embryos were recorded. Certainly, further studies are needed to investigate the molecular mechanisms underlying these promising biological effects, but considering the evidence of the present study, the use of CLJ as a ready-to drink safe prevention strategy for inflammatory-based diseases correlated to angiogenesis could be justified. |
| first_indexed | 2024-12-22T19:52:57Z |
| format | Article |
| id | doaj.art-0fede0d0ddaf45299c59612be6cadfe0 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-22T19:52:57Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-0fede0d0ddaf45299c59612be6cadfe02022-12-21T18:14:29ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-12-011110.3389/fphar.2020.593506593506Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia JuiceAntonella SmeriglioMarcella DenaroValeria D’AngeloMaria Paola GermanòDomenico TrombettaCitrus juices are a rich source of bioactive compounds with various and well-known health benefits. The aim of this study was to investigate the polyphenols and ascorbic acid content as well as to investigate the antioxidant, anti-inflammatory and anti-angiogenic properties of the juice of an ancient Mediterranean species, Citrus lumia Risso (CLJ). The antioxidant and anti-inflammatory activities were evaluated by several in vitro cell-free and cell-based assays, whereas two different in vivo models, the chick chorioallantoic membrane (CAM) and the zebrafish embryos, were used to characterize the anti-angiogenic properties. Twenty-eight polyphenols were identified by RP-LC-DAD-ESI-MS analysis (flavonoids 68.82% and phenolic acids 31.18%) with 1-caffeoyl-5-feruloylquinic acid and kaempferol 3′-rhamnoside, which represent the most abundant compounds (25.70 and 23.12%, respectively). HPLC-DAD analysis showed a high ascorbic acid content (352 mg/kg of CLJ), which contributes with polyphenols to the marked and dose-dependent antioxidant and anti-inflammatory properties observed. CLJ showed strong and dose-dependent anti-angiogenic activity as highlighted by the inhibition of blood vessel formation on CAMs and the decrease of endogenous alkaline phosphatase on zebrafish embryos. Moreover, within the concentration range tested, no dead or malformed embryos were recorded. Certainly, further studies are needed to investigate the molecular mechanisms underlying these promising biological effects, but considering the evidence of the present study, the use of CLJ as a ready-to drink safe prevention strategy for inflammatory-based diseases correlated to angiogenesis could be justified.https://www.frontiersin.org/articles/10.3389/fphar.2020.593506/fullcitrus juiceantioxidantanti-inflammatoryanti-angiogenicpolyphenolsascorbic acid |
| spellingShingle | Antonella Smeriglio Marcella Denaro Valeria D’Angelo Maria Paola Germanò Domenico Trombetta Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice Frontiers in Pharmacology citrus juice antioxidant anti-inflammatory anti-angiogenic polyphenols ascorbic acid |
| title | Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice |
| title_full | Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice |
| title_fullStr | Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice |
| title_full_unstemmed | Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice |
| title_short | Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice |
| title_sort | antioxidant anti inflammatory and anti angiogenic properties of citrus lumia juice |
| topic | citrus juice antioxidant anti-inflammatory anti-angiogenic polyphenols ascorbic acid |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2020.593506/full |
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