Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament
IntroductionThe lifelong eruption places a great demand on the dental pulp and periodontal ligament (PDL) of horse teeth. Cells within the pulp and PDL seem to play a key role during this remodeling.MethodsIn this study, we isolated and cultivated MSCs (medicinal signaling cells) from dental pulp, P...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-03-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1116671/full |
| _version_ | 1811155397952667648 |
|---|---|
| author | Laura Beate Heilen Jessica Roßgardt Jutta Dern-Wieloch Jörg Vogelsberg Carsten Staszyk |
| author_facet | Laura Beate Heilen Jessica Roßgardt Jutta Dern-Wieloch Jörg Vogelsberg Carsten Staszyk |
| author_sort | Laura Beate Heilen |
| collection | DOAJ |
| description | IntroductionThe lifelong eruption places a great demand on the dental pulp and periodontal ligament (PDL) of horse teeth. Cells within the pulp and PDL seem to play a key role during this remodeling.MethodsIn this study, we isolated and cultivated MSCs (medicinal signaling cells) from dental pulp, PDL and retrobulbar fat of four horses. Subsequently, we analyzed them by flow cytometry and immunohistochemistry to determine and compare their characteristics. In addition, we localized these cells within the tissue structure via immunohistochemistry of histological sections. For these analyses, several surface markers were applied.ResultsThe described method illustrates a feasible approach to isolate and cultivate MSCs from equine dental pulp and PDL. In the flow cytometry a vast majority of cultivated cells were positive for CD90 and CD40 and negative for CD11a/18, CD45, CD105 and MHCII suggesting that these cells feature characteristics of MSCs. Immunohistochemistry of histological pulp and PDL sections showed the localization of CD90 positive cells especially in the perivascular region and the subodontoblastic layer.DiscussionOur findings indicate that the isolation and cultivation of MSCs from equine dental pulp and PDL is feasible although an elaborate and complicated harvesting protocol is required. MSCs isolated from dental pulp and PDL are regarded as candidates for new therapeutical approaches in equine dental medicine like regeneration of periodontal lesions, enhancement of periodontal re-attachment after dental replantation and stimulation of pulp-obliteration and apexification in combination with endodontic therapies. |
| first_indexed | 2024-04-10T04:33:33Z |
| format | Article |
| id | doaj.art-0feed2c82b6c4ff18f9d094573a94a24 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T04:33:33Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-0feed2c82b6c4ff18f9d094573a94a242023-03-10T04:59:58ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-03-011010.3389/fvets.2023.11166711116671Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligamentLaura Beate HeilenJessica RoßgardtJutta Dern-WielochJörg VogelsbergCarsten StaszykIntroductionThe lifelong eruption places a great demand on the dental pulp and periodontal ligament (PDL) of horse teeth. Cells within the pulp and PDL seem to play a key role during this remodeling.MethodsIn this study, we isolated and cultivated MSCs (medicinal signaling cells) from dental pulp, PDL and retrobulbar fat of four horses. Subsequently, we analyzed them by flow cytometry and immunohistochemistry to determine and compare their characteristics. In addition, we localized these cells within the tissue structure via immunohistochemistry of histological sections. For these analyses, several surface markers were applied.ResultsThe described method illustrates a feasible approach to isolate and cultivate MSCs from equine dental pulp and PDL. In the flow cytometry a vast majority of cultivated cells were positive for CD90 and CD40 and negative for CD11a/18, CD45, CD105 and MHCII suggesting that these cells feature characteristics of MSCs. Immunohistochemistry of histological pulp and PDL sections showed the localization of CD90 positive cells especially in the perivascular region and the subodontoblastic layer.DiscussionOur findings indicate that the isolation and cultivation of MSCs from equine dental pulp and PDL is feasible although an elaborate and complicated harvesting protocol is required. MSCs isolated from dental pulp and PDL are regarded as candidates for new therapeutical approaches in equine dental medicine like regeneration of periodontal lesions, enhancement of periodontal re-attachment after dental replantation and stimulation of pulp-obliteration and apexification in combination with endodontic therapies.https://www.frontiersin.org/articles/10.3389/fvets.2023.1116671/fullhypsodont teethperiodontiumendodontiumequine dentistryregenerative medicineMSCs |
| spellingShingle | Laura Beate Heilen Jessica Roßgardt Jutta Dern-Wieloch Jörg Vogelsberg Carsten Staszyk Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament Frontiers in Veterinary Science hypsodont teeth periodontium endodontium equine dentistry regenerative medicine MSCs |
| title | Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament |
| title_full | Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament |
| title_fullStr | Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament |
| title_full_unstemmed | Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament |
| title_short | Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament |
| title_sort | isolation and cultivation as well as in situ identification of mscs from equine dental pulp and periodontal ligament |
| topic | hypsodont teeth periodontium endodontium equine dentistry regenerative medicine MSCs |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1116671/full |
| work_keys_str_mv | AT laurabeateheilen isolationandcultivationaswellasinsituidentificationofmscsfromequinedentalpulpandperiodontalligament AT jessicaroßgardt isolationandcultivationaswellasinsituidentificationofmscsfromequinedentalpulpandperiodontalligament AT juttadernwieloch isolationandcultivationaswellasinsituidentificationofmscsfromequinedentalpulpandperiodontalligament AT jorgvogelsberg isolationandcultivationaswellasinsituidentificationofmscsfromequinedentalpulpandperiodontalligament AT carstenstaszyk isolationandcultivationaswellasinsituidentificationofmscsfromequinedentalpulpandperiodontalligament |