Identification of Candidate Genes Involved in Bud Growth in <i>Pinus pinaster</i> through Knowledge Transfer from <i>Arabidopsis thaliana</i> Models
<i>Pinus pinaster</i> is a plant species of great ecological and economic importance. Understanding the underlying molecular mechanisms that govern the growth and branching of <i>P. pinaster</i> is crucial for enhancing wood production and improving product quality. In this s...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/9/1765 |
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| author | José Manuel Alvarez Sonia María Rodríguez Francisco Fuente-Maqueda Isabel Feito Ricardo Javier Ordás Candela Cuesta |
| author_facet | José Manuel Alvarez Sonia María Rodríguez Francisco Fuente-Maqueda Isabel Feito Ricardo Javier Ordás Candela Cuesta |
| author_sort | José Manuel Alvarez |
| collection | DOAJ |
| description | <i>Pinus pinaster</i> is a plant species of great ecological and economic importance. Understanding the underlying molecular mechanisms that govern the growth and branching of <i>P. pinaster</i> is crucial for enhancing wood production and improving product quality. In this study, we describe a simple methodology that enables the discovery of candidate genes in <i>Pinus pinaster</i> by transferring existing knowledge from model species like <i>Arabidopsis thaliana</i> and focusing on factors involved in plant growth, including hormonal and non-hormonal pathways. Through comparative analysis, we investigated the main genes associated with these growth-related factors in <i>A. thaliana</i>. Subsequently, we identified putative homologous sequences in <i>P. pinaster</i> and assessed the conservation of their functional domains. In this manner, we can exclude sequences that, despite displaying high homology, lack functional domains. Finally, we took an initial approach to their validation by examining the expression levels of these genes in <i>P. pinaster</i> trees exhibiting contrasting growth patterns. This methodology allowed the identification of 26 candidate genes in <i>P. pinaster</i>. Our findings revealed differential expression patterns of key genes, such as <i>NCED3</i>, <i>NRT1.2</i>, <i>PIN1</i>, <i>PP2A</i>, <i>ARF7</i>, <i>MAX1</i>, <i>MAX2</i>, <i>GID1</i>, <i>AHK4</i>, <i>AHP1</i>, and <i>STP1</i>, in relation to the different growth patterns analyzed. This study provides a methodological foundation for further exploration of these genes involved in the growth and branching processes of <i>P. pinaster</i>. This will contribute to the understanding of this important tree species and open new avenues for enhancing its utilization in sustainable forestry practices. |
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| last_indexed | 2024-03-10T22:45:01Z |
| publishDate | 2023-08-01 |
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| series | Forests |
| spelling | doaj.art-0ff27d8ee6ae4d0384cb8df52be15a1c2023-11-19T10:45:39ZengMDPI AGForests1999-49072023-08-01149176510.3390/f14091765Identification of Candidate Genes Involved in Bud Growth in <i>Pinus pinaster</i> through Knowledge Transfer from <i>Arabidopsis thaliana</i> ModelsJosé Manuel Alvarez0Sonia María Rodríguez1Francisco Fuente-Maqueda2Isabel Feito3Ricardo Javier Ordás4Candela Cuesta5Área de Fisiología Vegetal, Departamento de Biología de Organismos y Sistemas, Instituto Universitario de Biotecnología de Asturias (IUBA), Universidad de Oviedo, 33071 Oviedo, SpainÁrea de Fisiología Vegetal, Departamento de Biología de Organismos y Sistemas, Instituto Universitario de Biotecnología de Asturias (IUBA), Universidad de Oviedo, 33071 Oviedo, SpainPrograma Forestal, Área de Cultivos Hortofrutícolas y Forestales, Servicio Regional de Investigación y Desarrollo Agroalimentario de Asturias (SERIDA), Finca Experimental La Mata, 33820 Grado, SpainPrograma Forestal, Área de Cultivos Hortofrutícolas y Forestales, Servicio Regional de Investigación y Desarrollo Agroalimentario de Asturias (SERIDA), Finca Experimental La Mata, 33820 Grado, SpainÁrea de Fisiología Vegetal, Departamento de Biología de Organismos y Sistemas, Instituto Universitario de Biotecnología de Asturias (IUBA), Universidad de Oviedo, 33071 Oviedo, SpainÁrea de Fisiología Vegetal, Departamento de Biología de Organismos y Sistemas, Instituto Universitario de Biotecnología de Asturias (IUBA), Universidad de Oviedo, 33071 Oviedo, Spain<i>Pinus pinaster</i> is a plant species of great ecological and economic importance. Understanding the underlying molecular mechanisms that govern the growth and branching of <i>P. pinaster</i> is crucial for enhancing wood production and improving product quality. In this study, we describe a simple methodology that enables the discovery of candidate genes in <i>Pinus pinaster</i> by transferring existing knowledge from model species like <i>Arabidopsis thaliana</i> and focusing on factors involved in plant growth, including hormonal and non-hormonal pathways. Through comparative analysis, we investigated the main genes associated with these growth-related factors in <i>A. thaliana</i>. Subsequently, we identified putative homologous sequences in <i>P. pinaster</i> and assessed the conservation of their functional domains. In this manner, we can exclude sequences that, despite displaying high homology, lack functional domains. Finally, we took an initial approach to their validation by examining the expression levels of these genes in <i>P. pinaster</i> trees exhibiting contrasting growth patterns. This methodology allowed the identification of 26 candidate genes in <i>P. pinaster</i>. Our findings revealed differential expression patterns of key genes, such as <i>NCED3</i>, <i>NRT1.2</i>, <i>PIN1</i>, <i>PP2A</i>, <i>ARF7</i>, <i>MAX1</i>, <i>MAX2</i>, <i>GID1</i>, <i>AHK4</i>, <i>AHP1</i>, and <i>STP1</i>, in relation to the different growth patterns analyzed. This study provides a methodological foundation for further exploration of these genes involved in the growth and branching processes of <i>P. pinaster</i>. This will contribute to the understanding of this important tree species and open new avenues for enhancing its utilization in sustainable forestry practices.https://www.mdpi.com/1999-4907/14/9/1765<i>Pinus pinaster</i>growth regulatorsgrowthRT-qPCRbranching |
| spellingShingle | José Manuel Alvarez Sonia María Rodríguez Francisco Fuente-Maqueda Isabel Feito Ricardo Javier Ordás Candela Cuesta Identification of Candidate Genes Involved in Bud Growth in <i>Pinus pinaster</i> through Knowledge Transfer from <i>Arabidopsis thaliana</i> Models Forests <i>Pinus pinaster</i> growth regulators growth RT-qPCR branching |
| title | Identification of Candidate Genes Involved in Bud Growth in <i>Pinus pinaster</i> through Knowledge Transfer from <i>Arabidopsis thaliana</i> Models |
| title_full | Identification of Candidate Genes Involved in Bud Growth in <i>Pinus pinaster</i> through Knowledge Transfer from <i>Arabidopsis thaliana</i> Models |
| title_fullStr | Identification of Candidate Genes Involved in Bud Growth in <i>Pinus pinaster</i> through Knowledge Transfer from <i>Arabidopsis thaliana</i> Models |
| title_full_unstemmed | Identification of Candidate Genes Involved in Bud Growth in <i>Pinus pinaster</i> through Knowledge Transfer from <i>Arabidopsis thaliana</i> Models |
| title_short | Identification of Candidate Genes Involved in Bud Growth in <i>Pinus pinaster</i> through Knowledge Transfer from <i>Arabidopsis thaliana</i> Models |
| title_sort | identification of candidate genes involved in bud growth in i pinus pinaster i through knowledge transfer from i arabidopsis thaliana i models |
| topic | <i>Pinus pinaster</i> growth regulators growth RT-qPCR branching |
| url | https://www.mdpi.com/1999-4907/14/9/1765 |
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