Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study
Background:Staphylococcus aureus is a major human pathogen. Panton-Valentine leukocidin (PVL) is a virulence factor produced by some strains that causes leukocyte lysis and tissue necrosis. PVL-associated S. aureus (PVL-SA) predominantly causes skin and soft-tissue infections (SSTIs) but can also ca...
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2019.00170/full |
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| author | Saffiatou Darboe Sarah Dobreniecki Sheikh Jarju Mamadou Jallow Nuredin Ibrahim Mohammed Miriam Wathuo Buntung Ceesay Sam Tweed Robindra Basu Roy Uduak Okomo Brenda Kwambana-Adams Martin Antonio Richard S. Bradbury Thushan I. de Silva Karen Forrest Anna Roca Bolarinde Joseph Lawal Davis Nwakanma Ousman Secka |
| author_facet | Saffiatou Darboe Sarah Dobreniecki Sheikh Jarju Mamadou Jallow Nuredin Ibrahim Mohammed Miriam Wathuo Buntung Ceesay Sam Tweed Robindra Basu Roy Uduak Okomo Brenda Kwambana-Adams Martin Antonio Richard S. Bradbury Thushan I. de Silva Karen Forrest Anna Roca Bolarinde Joseph Lawal Davis Nwakanma Ousman Secka |
| author_sort | Saffiatou Darboe |
| collection | DOAJ |
| description | Background:Staphylococcus aureus is a major human pathogen. Panton-Valentine leukocidin (PVL) is a virulence factor produced by some strains that causes leukocyte lysis and tissue necrosis. PVL-associated S. aureus (PVL-SA) predominantly causes skin and soft-tissue infections (SSTIs) but can also cause invasive infections such as necrotizing pneumonia. It is carried by both community-associated methicillin susceptible S. aureus (CA-MSSA) and methicillin resistant S. aureus (CA-MRSA). This study aims to determine the prevalence of PVL-SA among patients seen at an urban Gambian hospital and associated antibiotic resistance.Methods: Archived clinical S. aureus (70 invasive bacteraemia and 223 non-invasive SSTIs) from 293 patients were retrieved as well as relevant data from clinical records where available. Antibiotic susceptibility was assessed using disc diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines. Genomic DNA was extracted and the presence of lukF and lukS PVL genes was detected by conventional gel-based PCR.Result: PVL-SA strains accounted for 61.4% (180/293) of S. aureus isolates. PVL prevalence was high in both Gambian bacteraemia and SSTIs S. aureus strains. Antimicrobial resistance was low and included chloramphenicol (4.8%), cefoxitin (2.4%), ciprofloxacin (3.8%), erythromycin (8.9%), gentamicin (5.5%) penicillin (92.5%), tetracycline (41.0%), and sulfamethoxazole-trimethoprim (24.2%). There was no association of PVL with antimicrobial resistance.Conclusion: PVL expression is high among clinical S. aureus strains among Gambian patients. Reporting of PVL-SA clinical infections is necessary to enable the monitoring of the clinical impact of these strains in the population and guide prevention of the spread of virulent PVL-positive CA-MRSA strains.SUMMARY Staphylococcus aureus (S. aureus) is a major human pathogen with several virulence factors. We performed a retrospective analysis to investigate the prevalence of one such virulence factor (PVL) amongst clinical S. aureus samples. We found a high prevalence in our setting but antimicrobial resistance including methicillin resistance was low. |
| first_indexed | 2024-12-13T04:16:16Z |
| format | Article |
| id | doaj.art-0ff408155102417f9d39cf0c1f72e7fc |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-13T04:16:16Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-0ff408155102417f9d39cf0c1f72e7fc2022-12-21T23:59:53ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882019-05-01910.3389/fcimb.2019.00170455674Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot StudySaffiatou Darboe0Sarah Dobreniecki1Sheikh Jarju2Mamadou Jallow3Nuredin Ibrahim Mohammed4Miriam Wathuo5Buntung Ceesay6Sam Tweed7Robindra Basu Roy8Uduak Okomo9Brenda Kwambana-Adams10Martin Antonio11Richard S. Bradbury12Thushan I. de Silva13Karen Forrest14Anna Roca15Bolarinde Joseph Lawal16Davis Nwakanma17Ousman Secka18Medical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaEnvironmental Protection Agency, Washington, DC, United StatesMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaThe School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, United KingdomLondon School of Hygiene and Tropical Medicine, London, United KingdomLondon School of Hygiene and Tropical Medicine, London, United KingdomMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaSchool of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD, AustraliaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaMedical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, GambiaBackground:Staphylococcus aureus is a major human pathogen. Panton-Valentine leukocidin (PVL) is a virulence factor produced by some strains that causes leukocyte lysis and tissue necrosis. PVL-associated S. aureus (PVL-SA) predominantly causes skin and soft-tissue infections (SSTIs) but can also cause invasive infections such as necrotizing pneumonia. It is carried by both community-associated methicillin susceptible S. aureus (CA-MSSA) and methicillin resistant S. aureus (CA-MRSA). This study aims to determine the prevalence of PVL-SA among patients seen at an urban Gambian hospital and associated antibiotic resistance.Methods: Archived clinical S. aureus (70 invasive bacteraemia and 223 non-invasive SSTIs) from 293 patients were retrieved as well as relevant data from clinical records where available. Antibiotic susceptibility was assessed using disc diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines. Genomic DNA was extracted and the presence of lukF and lukS PVL genes was detected by conventional gel-based PCR.Result: PVL-SA strains accounted for 61.4% (180/293) of S. aureus isolates. PVL prevalence was high in both Gambian bacteraemia and SSTIs S. aureus strains. Antimicrobial resistance was low and included chloramphenicol (4.8%), cefoxitin (2.4%), ciprofloxacin (3.8%), erythromycin (8.9%), gentamicin (5.5%) penicillin (92.5%), tetracycline (41.0%), and sulfamethoxazole-trimethoprim (24.2%). There was no association of PVL with antimicrobial resistance.Conclusion: PVL expression is high among clinical S. aureus strains among Gambian patients. Reporting of PVL-SA clinical infections is necessary to enable the monitoring of the clinical impact of these strains in the population and guide prevention of the spread of virulent PVL-positive CA-MRSA strains.SUMMARY Staphylococcus aureus (S. aureus) is a major human pathogen with several virulence factors. We performed a retrospective analysis to investigate the prevalence of one such virulence factor (PVL) amongst clinical S. aureus samples. We found a high prevalence in our setting but antimicrobial resistance including methicillin resistance was low.https://www.frontiersin.org/article/10.3389/fcimb.2019.00170/fullPanton-Valentine leukocidinStaphylococcus aureuscommunity-acquiredantimicrobial resistanceThe Gambia |
| spellingShingle | Saffiatou Darboe Sarah Dobreniecki Sheikh Jarju Mamadou Jallow Nuredin Ibrahim Mohammed Miriam Wathuo Buntung Ceesay Sam Tweed Robindra Basu Roy Uduak Okomo Brenda Kwambana-Adams Martin Antonio Richard S. Bradbury Thushan I. de Silva Karen Forrest Anna Roca Bolarinde Joseph Lawal Davis Nwakanma Ousman Secka Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study Frontiers in Cellular and Infection Microbiology Panton-Valentine leukocidin Staphylococcus aureus community-acquired antimicrobial resistance The Gambia |
| title | Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study |
| title_full | Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study |
| title_fullStr | Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study |
| title_full_unstemmed | Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study |
| title_short | Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study |
| title_sort | prevalence of panton valentine leukocidin pvl and antimicrobial resistance in community acquired clinical staphylococcus aureus in an urban gambian hospital a 11 year period retrospective pilot study |
| topic | Panton-Valentine leukocidin Staphylococcus aureus community-acquired antimicrobial resistance The Gambia |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2019.00170/full |
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