Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity
Capparis spinosa is cultivated for several proprieties and the different parts of the plant (flower buds, fruits, leaf and seeds) have been employed in drugs, foods and cosmetics. This study aimed to explore the antioxidant potential effect and to assess the anthelmintic efficacy of an aqueous extra...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2016-06-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201606-0002_potential-anthelmintic-effect-of-capparis-spinosa-capparidaceae-as-related-to-its-polyphenolic-content-and-an.php |
| _version_ | 1797897491766575104 |
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| author | H. Akkari F. B'chir S. Hajaji M. Rekik E. Sebai H. Hamza M.A. Darghouth M. Gharbi |
| author_facet | H. Akkari F. B'chir S. Hajaji M. Rekik E. Sebai H. Hamza M.A. Darghouth M. Gharbi |
| author_sort | H. Akkari |
| collection | DOAJ |
| description | Capparis spinosa is cultivated for several proprieties and the different parts of the plant (flower buds, fruits, leaf and seeds) have been employed in drugs, foods and cosmetics. This study aimed to explore the antioxidant potential effect and to assess the anthelmintic efficacy of an aqueous extract of Capparis spinosa. In vitro antioxidant activity of aqueous extracts from the fresh leaves and flower buds of C. spinosa was measured by determining free radical-scavenging activity against DPPH and ABTS radical cations. In vitro anthelmintic activities against eggs and adult worms of Haemonchus contortus from sheep were investigated in comparison to albendazole. DPPH and ABTS activities of both aqueous extracts increased in a dose dependent manner. The IC50 values of aqueous extracts from fresh leaves, flowers buds and ascorbic acid in the DPPH radical scavenging assay were 101.40; 70.40 and 57.56 μg/ml, respectively. The IC50 values of aqueous extracts fresh leaves, flowers buds and ascorbic acid in the ABTS radical scavenging assay were 110; 87.89 and 58.17 μg/ml, respectively. Both plant extracts showed ovicidal activity at all tested concentrations. The aqueous extract from flower buds (IC50 = 2.76 mg/ml) showed higher inhibitory effects (P < 0.05) than aqueous extract from leaves (IC50 = 8.54 mg/ml) in the egg hatching assay. The aqueous extract from flower buds inhibited more worms than the aqueous extract from leaves at all tested concentrations. After 6 h of exposure, the highest tested concentrations of aqueous extracts from flower buds and leaves induced 100 and 41.66% inhibition of motility, respectively. These results show for the first time that C. spinosa possesses in vitro anthelmintic properties which may be related to its high content of phenolic compounds such as flavonoids and tannins. |
| first_indexed | 2024-04-10T07:59:18Z |
| format | Article |
| id | doaj.art-0ff6737a69414566902b6cff03dfe6b4 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:59:18Z |
| publishDate | 2016-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-0ff6737a69414566902b6cff03dfe6b42023-02-23T03:50:32ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922016-06-0161630831610.17221/169/2015-VETMEDvet-201606-0002Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activityH. Akkari0F. B'chir1S. Hajaji2M. Rekik3E. Sebai4H. Hamza5M.A. Darghouth6M. Gharbi7National School of Veterinary Medicine, University of Manouba, Sidi Thabet, TunisiaInstitut National de Recherche et d'Analyse Physico-Chimique-Pole Technologique de Sidi Thabet, Sidi Thabet, TunisiaNational School of Veterinary Medicine, University of Manouba, Sidi Thabet, TunisiaInternational Center for Agricultural Research in the Dry Areas (ICARDA), Amman, JordanNational School of Veterinary Medicine, University of Manouba, Sidi Thabet, TunisiaNational School of Veterinary Medicine, University of Manouba, Sidi Thabet, TunisiaNational School of Veterinary Medicine, University of Manouba, Sidi Thabet, TunisiaNational School of Veterinary Medicine, University of Manouba, Sidi Thabet, TunisiaCapparis spinosa is cultivated for several proprieties and the different parts of the plant (flower buds, fruits, leaf and seeds) have been employed in drugs, foods and cosmetics. This study aimed to explore the antioxidant potential effect and to assess the anthelmintic efficacy of an aqueous extract of Capparis spinosa. In vitro antioxidant activity of aqueous extracts from the fresh leaves and flower buds of C. spinosa was measured by determining free radical-scavenging activity against DPPH and ABTS radical cations. In vitro anthelmintic activities against eggs and adult worms of Haemonchus contortus from sheep were investigated in comparison to albendazole. DPPH and ABTS activities of both aqueous extracts increased in a dose dependent manner. The IC50 values of aqueous extracts from fresh leaves, flowers buds and ascorbic acid in the DPPH radical scavenging assay were 101.40; 70.40 and 57.56 μg/ml, respectively. The IC50 values of aqueous extracts fresh leaves, flowers buds and ascorbic acid in the ABTS radical scavenging assay were 110; 87.89 and 58.17 μg/ml, respectively. Both plant extracts showed ovicidal activity at all tested concentrations. The aqueous extract from flower buds (IC50 = 2.76 mg/ml) showed higher inhibitory effects (P < 0.05) than aqueous extract from leaves (IC50 = 8.54 mg/ml) in the egg hatching assay. The aqueous extract from flower buds inhibited more worms than the aqueous extract from leaves at all tested concentrations. After 6 h of exposure, the highest tested concentrations of aqueous extracts from flower buds and leaves induced 100 and 41.66% inhibition of motility, respectively. These results show for the first time that C. spinosa possesses in vitro anthelmintic properties which may be related to its high content of phenolic compounds such as flavonoids and tannins.https://vetmed.agriculturejournals.cz/artkey/vet-201606-0002_potential-anthelmintic-effect-of-capparis-spinosa-capparidaceae-as-related-to-its-polyphenolic-content-and-an.phpcapparis spinosafree radical-scavenging abilityanthelmintichaemonchus contortus |
| spellingShingle | H. Akkari F. B'chir S. Hajaji M. Rekik E. Sebai H. Hamza M.A. Darghouth M. Gharbi Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity Veterinární Medicína capparis spinosa free radical-scavenging ability anthelmintic haemonchus contortus |
| title | Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity |
| title_full | Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity |
| title_fullStr | Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity |
| title_full_unstemmed | Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity |
| title_short | Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity |
| title_sort | potential anthelmintic effect of capparis spinosa capparidaceae as related to its polyphenolic content and antioxidant activity |
| topic | capparis spinosa free radical-scavenging ability anthelmintic haemonchus contortus |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201606-0002_potential-anthelmintic-effect-of-capparis-spinosa-capparidaceae-as-related-to-its-polyphenolic-content-and-an.php |
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