Investigation of Therapeutic Response Markers for Acupuncture in Parkinson’s Disease: An Exploratory Pilot Study
In this preliminary pilot study, we investigated the specific genes implicated in the therapeutic response to acupuncture in patients with Parkinson’s disease (PD). Transcriptome alterations following acupuncture in blood samples collected during our previous clinical trial were analyzed along with...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/9/1697 |
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| author | Sang-Min Park Aeyung Kim Gunhyuk Park Ojin Kwon Sangsoo Park Horyong Yoo Jung-Hee Jang |
| author_facet | Sang-Min Park Aeyung Kim Gunhyuk Park Ojin Kwon Sangsoo Park Horyong Yoo Jung-Hee Jang |
| author_sort | Sang-Min Park |
| collection | DOAJ |
| description | In this preliminary pilot study, we investigated the specific genes implicated in the therapeutic response to acupuncture in patients with Parkinson’s disease (PD). Transcriptome alterations following acupuncture in blood samples collected during our previous clinical trial were analyzed along with the clinical data of six patients with PD, of which a representative patient was selected for transcriptomic analysis following acupuncture. We also examined the changes in the expression of PD biomarker genes known to be dysregulated in both the brain and blood of patients with PD. We validated these gene expression changes using quantitative real-time polymerase chain reaction (qPCR) in the blood of the remaining five patients with PD who received acupuncture treatment. Following acupuncture treatment, the transcriptomic alterations in the representative patient were similar to those induced by dopaminergic therapy. Among the PD biomarkers, ankyrin repeat domain 22 (ANKRD22), upregulated following dopaminergic therapy, and synapsin 1 (SYN1), a common gene marker for synaptic dysfunction in PD, were upregulated following acupuncture. These alterations correlated with changes in gait parameters in patients with PD. Our data suggest ANKRD22 and SYN1 as potential biomarkers to predict/monitor therapeutic responses to acupuncture in patients with PD, especially in those with gait disturbance. Further research is needed to confirm these findings in a large sample of patients with PD. |
| first_indexed | 2024-03-10T07:45:09Z |
| format | Article |
| id | doaj.art-0ffa234cf2914da2bfb4a06a0eed8903 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T07:45:09Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-0ffa234cf2914da2bfb4a06a0eed89032023-11-22T12:41:00ZengMDPI AGDiagnostics2075-44182021-09-01119169710.3390/diagnostics11091697Investigation of Therapeutic Response Markers for Acupuncture in Parkinson’s Disease: An Exploratory Pilot StudySang-Min Park0Aeyung Kim1Gunhyuk Park2Ojin Kwon3Sangsoo Park4Horyong Yoo5Jung-Hee Jang6KM Data Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 34054, KoreaKM Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, KoreaHerbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111 Geonjae-ro, Naju-si 58245, KoreaKM Science Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 34054, KoreaClinical Trial Center, Daejeon Korean Medicine Hospital, 75, Daedeok-Daero 176 Beon-gil, Seo-gu, Daejeon 35235, KoreaClinical Trial Center, Daejeon Korean Medicine Hospital, 75, Daedeok-Daero 176 Beon-gil, Seo-gu, Daejeon 35235, KoreaKM Science Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 34054, KoreaIn this preliminary pilot study, we investigated the specific genes implicated in the therapeutic response to acupuncture in patients with Parkinson’s disease (PD). Transcriptome alterations following acupuncture in blood samples collected during our previous clinical trial were analyzed along with the clinical data of six patients with PD, of which a representative patient was selected for transcriptomic analysis following acupuncture. We also examined the changes in the expression of PD biomarker genes known to be dysregulated in both the brain and blood of patients with PD. We validated these gene expression changes using quantitative real-time polymerase chain reaction (qPCR) in the blood of the remaining five patients with PD who received acupuncture treatment. Following acupuncture treatment, the transcriptomic alterations in the representative patient were similar to those induced by dopaminergic therapy. Among the PD biomarkers, ankyrin repeat domain 22 (ANKRD22), upregulated following dopaminergic therapy, and synapsin 1 (SYN1), a common gene marker for synaptic dysfunction in PD, were upregulated following acupuncture. These alterations correlated with changes in gait parameters in patients with PD. Our data suggest ANKRD22 and SYN1 as potential biomarkers to predict/monitor therapeutic responses to acupuncture in patients with PD, especially in those with gait disturbance. Further research is needed to confirm these findings in a large sample of patients with PD.https://www.mdpi.com/2075-4418/11/9/1697Parkinson’s diseaseacupuncturetranscriptomeSYN1ANKRD22 |
| spellingShingle | Sang-Min Park Aeyung Kim Gunhyuk Park Ojin Kwon Sangsoo Park Horyong Yoo Jung-Hee Jang Investigation of Therapeutic Response Markers for Acupuncture in Parkinson’s Disease: An Exploratory Pilot Study Diagnostics Parkinson’s disease acupuncture transcriptome SYN1 ANKRD22 |
| title | Investigation of Therapeutic Response Markers for Acupuncture in Parkinson’s Disease: An Exploratory Pilot Study |
| title_full | Investigation of Therapeutic Response Markers for Acupuncture in Parkinson’s Disease: An Exploratory Pilot Study |
| title_fullStr | Investigation of Therapeutic Response Markers for Acupuncture in Parkinson’s Disease: An Exploratory Pilot Study |
| title_full_unstemmed | Investigation of Therapeutic Response Markers for Acupuncture in Parkinson’s Disease: An Exploratory Pilot Study |
| title_short | Investigation of Therapeutic Response Markers for Acupuncture in Parkinson’s Disease: An Exploratory Pilot Study |
| title_sort | investigation of therapeutic response markers for acupuncture in parkinson s disease an exploratory pilot study |
| topic | Parkinson’s disease acupuncture transcriptome SYN1 ANKRD22 |
| url | https://www.mdpi.com/2075-4418/11/9/1697 |
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