Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap
Elucidation of cell-level transport mediated by vesicles within a living cell provides key information regarding viral infection processes and also drug delivery mechanisms. Although the single-particle tracking method has enabled clear analysis of individual vesicle trajectories, information regard...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/21/2/522 |
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| author | Seohyun Lee Hyuno Kim Hideo Higuchi Masatoshi Ishikawa |
| author_facet | Seohyun Lee Hyuno Kim Hideo Higuchi Masatoshi Ishikawa |
| author_sort | Seohyun Lee |
| collection | DOAJ |
| description | Elucidation of cell-level transport mediated by vesicles within a living cell provides key information regarding viral infection processes and also drug delivery mechanisms. Although the single-particle tracking method has enabled clear analysis of individual vesicle trajectories, information regarding the entire cell-level intracellular transport is hardly obtainable, due to the difficulty in collecting a large dataset with current methods. In this paper, we propose a visualization method of vesicle transport using optical flow, based on geometric cell center estimation and vector analysis, for measuring the trafficking directions. As a quantitative visualization method for determining the intracellular transport status, the proposed method is expected to be universally exploited in various biomedical cell image analyses. |
| first_indexed | 2024-03-09T04:56:50Z |
| format | Article |
| id | doaj.art-0ffc19ce1f6c4dfbb29576ee3ae5fdbe |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T04:56:50Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-0ffc19ce1f6c4dfbb29576ee3ae5fdbe2023-12-03T13:03:54ZengMDPI AGSensors1424-82202021-01-0121252210.3390/s21020522Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging ColormapSeohyun Lee0Hyuno Kim1Hideo Higuchi2Masatoshi Ishikawa3Information Technology Center, Data Science Research Division, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8656, JapanInformation Technology Center, Data Science Research Division, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8656, JapanDepartment of Physics, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, JapanInformation Technology Center, Data Science Research Division, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8656, JapanElucidation of cell-level transport mediated by vesicles within a living cell provides key information regarding viral infection processes and also drug delivery mechanisms. Although the single-particle tracking method has enabled clear analysis of individual vesicle trajectories, information regarding the entire cell-level intracellular transport is hardly obtainable, due to the difficulty in collecting a large dataset with current methods. In this paper, we propose a visualization method of vesicle transport using optical flow, based on geometric cell center estimation and vector analysis, for measuring the trafficking directions. As a quantitative visualization method for determining the intracellular transport status, the proposed method is expected to be universally exploited in various biomedical cell image analyses.https://www.mdpi.com/1424-8220/21/2/522vesicle transportoptical flowvisualization |
| spellingShingle | Seohyun Lee Hyuno Kim Hideo Higuchi Masatoshi Ishikawa Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap Sensors vesicle transport optical flow visualization |
| title | Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap |
| title_full | Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap |
| title_fullStr | Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap |
| title_full_unstemmed | Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap |
| title_short | Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap |
| title_sort | visualization method for the cell level vesicle transport using optical flow and a diverging colormap |
| topic | vesicle transport optical flow visualization |
| url | https://www.mdpi.com/1424-8220/21/2/522 |
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