Adiponectin alleviate blood hypercoagulability via inhibiting endothelial cell apoptosis induced by oxidative stress in septic rats
Objective(s): The purpose of this study was to detect the protective effects of adiponectin on coagulation dysfunction and its mechanism in sepsis of rats.Materials and Methods: The experimental samples were composed of sham group, model group that was underwent cecal ligation and puncture (CLP) a...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-10-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_11356_79ab85f00fb2a251dbd2992742e18bcb.pdf |
| _version_ | 1828288978117197824 |
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| author | Yun Hou Xi-Feng Wang Zhi-Qiang Lang Wei Zhao Yinchuan Jin Hong-Qin Zhang Lian-Shuang Zhang |
| author_facet | Yun Hou Xi-Feng Wang Zhi-Qiang Lang Wei Zhao Yinchuan Jin Hong-Qin Zhang Lian-Shuang Zhang |
| author_sort | Yun Hou |
| collection | DOAJ |
| description | Objective(s): The purpose of this study was to detect the protective effects of adiponectin on coagulation dysfunction and its mechanism in sepsis of rats.Materials and Methods: The experimental samples were composed of sham group, model group that was underwent cecal ligation and puncture (CLP) and three adiponectin treatment groups that treated by adiponectin with different dose (72 μg/kg, 96 μg/kg and 120 μg/kg) after CLP. The prothrombin time (PT), activated partial thromboplastin time (APTT) was measured, respectively, the level of malondialdehyde (MDA), tissue factor (TF), activated coagulation factor VIIa and Xa, p-selectin were detected, the histology structure of vascular was observed, the expressions of Caspase 9, Caspase 3, Bax, Bcl-2 and vWF in vascular were measured.Results: The results demonstrated that adiponectin treatment lengthened PT and APTT, reduced the expression of MDA, TF, activated coagulation factor VIIa, Xa and p-selectin in plasma of septic rats. Additionally, adiponectin treatment alleviated endothelial cell apoptosis and oxidative stress, down-regulated the levels of Caspase 3, Caspase 9, Bax, Bcl-2 and vWF in vascular.Conclusion: These findings suggest that adiponectin treatment might be a promising therapeutic strategy for relieving septic endothelial cell injury and coagulation dysfunction via inhibiting endothelial cell apoptosis in septic rats. |
| first_indexed | 2024-04-13T10:12:39Z |
| format | Article |
| id | doaj.art-0ffef70c32114796af86b62d487bc696 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T10:12:39Z |
| publishDate | 2018-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-0ffef70c32114796af86b62d487bc6962022-12-22T02:50:49ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-10-0121101013101910.22038/ijbms.2018.29389.711711356Adiponectin alleviate blood hypercoagulability via inhibiting endothelial cell apoptosis induced by oxidative stress in septic ratsYun Hou0Xi-Feng Wang1Zhi-Qiang Lang2Wei Zhao3Yinchuan Jin4Hong-Qin Zhang5Lian-Shuang Zhang6Department of Histology and Embryology, Binzhou Medical University, Yantai, P R ChinaDepartment of Critical Care Medicine, Yu Huang Ding Hospital, Qingdao University, Yantai, P R ChinaDepartment of Pathology, Yu Huang Ding Hospital, Qingdao University, Yan Tai, P R ChinaDepartment of Histology and Embryology, Binzhou Medical University, Yantai, P R ChinaDepartment of Histology and Embryology, Binzhou Medical University, Yantai, P R ChinaDepartment of Histology and Embryology, Binzhou Medical University, Yantai, P R ChinaDepartment of Histology and Embryology, Binzhou Medical University, Yantai, P R ChinaObjective(s): The purpose of this study was to detect the protective effects of adiponectin on coagulation dysfunction and its mechanism in sepsis of rats.Materials and Methods: The experimental samples were composed of sham group, model group that was underwent cecal ligation and puncture (CLP) and three adiponectin treatment groups that treated by adiponectin with different dose (72 μg/kg, 96 μg/kg and 120 μg/kg) after CLP. The prothrombin time (PT), activated partial thromboplastin time (APTT) was measured, respectively, the level of malondialdehyde (MDA), tissue factor (TF), activated coagulation factor VIIa and Xa, p-selectin were detected, the histology structure of vascular was observed, the expressions of Caspase 9, Caspase 3, Bax, Bcl-2 and vWF in vascular were measured.Results: The results demonstrated that adiponectin treatment lengthened PT and APTT, reduced the expression of MDA, TF, activated coagulation factor VIIa, Xa and p-selectin in plasma of septic rats. Additionally, adiponectin treatment alleviated endothelial cell apoptosis and oxidative stress, down-regulated the levels of Caspase 3, Caspase 9, Bax, Bcl-2 and vWF in vascular.Conclusion: These findings suggest that adiponectin treatment might be a promising therapeutic strategy for relieving septic endothelial cell injury and coagulation dysfunction via inhibiting endothelial cell apoptosis in septic rats.http://ijbms.mums.ac.ir/article_11356_79ab85f00fb2a251dbd2992742e18bcb.pdfAdiponectinApoptosisEndothelial cellsOxidative stressRatsSepsisThrombophilia |
| spellingShingle | Yun Hou Xi-Feng Wang Zhi-Qiang Lang Wei Zhao Yinchuan Jin Hong-Qin Zhang Lian-Shuang Zhang Adiponectin alleviate blood hypercoagulability via inhibiting endothelial cell apoptosis induced by oxidative stress in septic rats Iranian Journal of Basic Medical Sciences Adiponectin Apoptosis Endothelial cells Oxidative stress Rats Sepsis Thrombophilia |
| title | Adiponectin alleviate blood hypercoagulability via inhibiting endothelial cell apoptosis induced by oxidative stress in septic rats |
| title_full | Adiponectin alleviate blood hypercoagulability via inhibiting endothelial cell apoptosis induced by oxidative stress in septic rats |
| title_fullStr | Adiponectin alleviate blood hypercoagulability via inhibiting endothelial cell apoptosis induced by oxidative stress in septic rats |
| title_full_unstemmed | Adiponectin alleviate blood hypercoagulability via inhibiting endothelial cell apoptosis induced by oxidative stress in septic rats |
| title_short | Adiponectin alleviate blood hypercoagulability via inhibiting endothelial cell apoptosis induced by oxidative stress in septic rats |
| title_sort | adiponectin alleviate blood hypercoagulability via inhibiting endothelial cell apoptosis induced by oxidative stress in septic rats |
| topic | Adiponectin Apoptosis Endothelial cells Oxidative stress Rats Sepsis Thrombophilia |
| url | http://ijbms.mums.ac.ir/article_11356_79ab85f00fb2a251dbd2992742e18bcb.pdf |
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