Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer
Renal cell carcinoma (RCC) represents around 3% of all cancers, within which clear cell RCC (ccRCC) are the most common type (70–75%). The RCC disease regularly progresses asymptomatically and upon presentation is recurrently metastatic, therefore, an early method of detection is necessary. The iden...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/16/4213 |
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| author | Michela Murdocca Francesco Torino Sabina Pucci Manuela Costantini Rosamaria Capuano Chiara Greggi Chiara Polidoro Giuseppina Somma Valentina Pasqualetti Yolande Ketchanji Mougang Alexandro Catini Giuseppe Simone Roberto Paolesse Augusto Orlandi Alessandro Mauriello Mario Roselli Andrea Magrini Giuseppe Novelli Corrado Di Natale Federica Carla Sangiuolo |
| author_facet | Michela Murdocca Francesco Torino Sabina Pucci Manuela Costantini Rosamaria Capuano Chiara Greggi Chiara Polidoro Giuseppina Somma Valentina Pasqualetti Yolande Ketchanji Mougang Alexandro Catini Giuseppe Simone Roberto Paolesse Augusto Orlandi Alessandro Mauriello Mario Roselli Andrea Magrini Giuseppe Novelli Corrado Di Natale Federica Carla Sangiuolo |
| author_sort | Michela Murdocca |
| collection | DOAJ |
| description | Renal cell carcinoma (RCC) represents around 3% of all cancers, within which clear cell RCC (ccRCC) are the most common type (70–75%). The RCC disease regularly progresses asymptomatically and upon presentation is recurrently metastatic, therefore, an early method of detection is necessary. The identification of one or more specific biomarkers measurable in biofluids (i.e., urine) by combined approaches could surely be appropriate for this kind of cancer, especially due to easy obtainability by noninvasive method. OLR1 is a metabolic gene that encodes for the Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), implicated in inflammation, atherosclerosis, ROS, and metabolic disorder-associated carcinogenesis. Specifically, LOX-1 is clearly involved in tumor insurgence and progression of different human cancers. This work reports for the first time the presence of LOX-1 protein in ccRCC urine and its peculiar distribution in tumoral tissues. The urine samples headspace has also been analyzed for the presence of the volatile compounds (VOCs) by SPME-GC/MS and gas sensor array. In particular, it was found by GC/MS analysis that 2-Cyclohexen-1-one,3-methyl-6-(1-methylethyl)- correlates with LOX-1 concentration in urine. The combined approach of VOCs analysis and protein quantification could lead to promising results in terms of diagnostic and prognostic potential for ccRCC tumors. |
| first_indexed | 2024-03-10T08:56:10Z |
| format | Article |
| id | doaj.art-100729d63143480e946ecb7432e5a423 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T08:56:10Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-100729d63143480e946ecb7432e5a4232023-11-22T07:05:30ZengMDPI AGCancers2072-66942021-08-011316421310.3390/cancers13164213Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal CancerMichela Murdocca0Francesco Torino1Sabina Pucci2Manuela Costantini3Rosamaria Capuano4Chiara Greggi5Chiara Polidoro6Giuseppina Somma7Valentina Pasqualetti8Yolande Ketchanji Mougang9Alexandro Catini10Giuseppe Simone11Roberto Paolesse12Augusto Orlandi13Alessandro Mauriello14Mario Roselli15Andrea Magrini16Giuseppe Novelli17Corrado Di Natale18Federica Carla Sangiuolo19Department of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of System Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of Urology, IRCCS Regina Elena National Cancer Institute, 00144 Rome, ItalyDepartment of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, ItalyDepartment of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, ItalyDepartment of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, ItalyDepartment of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, ItalyDepartment of Urology, IRCCS Regina Elena National Cancer Institute, 00144 Rome, ItalyDepartment of Chemical Science and Technology, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, ItalyDepartment of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment Experimental Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of System Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, ItalyDepartment of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyRenal cell carcinoma (RCC) represents around 3% of all cancers, within which clear cell RCC (ccRCC) are the most common type (70–75%). The RCC disease regularly progresses asymptomatically and upon presentation is recurrently metastatic, therefore, an early method of detection is necessary. The identification of one or more specific biomarkers measurable in biofluids (i.e., urine) by combined approaches could surely be appropriate for this kind of cancer, especially due to easy obtainability by noninvasive method. OLR1 is a metabolic gene that encodes for the Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), implicated in inflammation, atherosclerosis, ROS, and metabolic disorder-associated carcinogenesis. Specifically, LOX-1 is clearly involved in tumor insurgence and progression of different human cancers. This work reports for the first time the presence of LOX-1 protein in ccRCC urine and its peculiar distribution in tumoral tissues. The urine samples headspace has also been analyzed for the presence of the volatile compounds (VOCs) by SPME-GC/MS and gas sensor array. In particular, it was found by GC/MS analysis that 2-Cyclohexen-1-one,3-methyl-6-(1-methylethyl)- correlates with LOX-1 concentration in urine. The combined approach of VOCs analysis and protein quantification could lead to promising results in terms of diagnostic and prognostic potential for ccRCC tumors.https://www.mdpi.com/2072-6694/13/16/4213clear cell renal cell carcinoma (ccRCC)LOX-1 proteinvolatile compounds (VOCs)prognostic biomarkerurinegas chromatography mass spectrometer (GC/MS) |
| spellingShingle | Michela Murdocca Francesco Torino Sabina Pucci Manuela Costantini Rosamaria Capuano Chiara Greggi Chiara Polidoro Giuseppina Somma Valentina Pasqualetti Yolande Ketchanji Mougang Alexandro Catini Giuseppe Simone Roberto Paolesse Augusto Orlandi Alessandro Mauriello Mario Roselli Andrea Magrini Giuseppe Novelli Corrado Di Natale Federica Carla Sangiuolo Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer Cancers clear cell renal cell carcinoma (ccRCC) LOX-1 protein volatile compounds (VOCs) prognostic biomarker urine gas chromatography mass spectrometer (GC/MS) |
| title | Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer |
| title_full | Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer |
| title_fullStr | Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer |
| title_full_unstemmed | Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer |
| title_short | Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer |
| title_sort | urine lox 1 and volatilome as promising tools towards the early detection of renal cancer |
| topic | clear cell renal cell carcinoma (ccRCC) LOX-1 protein volatile compounds (VOCs) prognostic biomarker urine gas chromatography mass spectrometer (GC/MS) |
| url | https://www.mdpi.com/2072-6694/13/16/4213 |
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