Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation
Surface structures and physicochemical properties critically influence osseointegration of titanium (Ti) implants. Previous studies have shown that the surface with both micro- and nanoscale roughness may provide multiple features comparable to cell dimensions and thus efficiently regulate cell-mate...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2021-04-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X20302565 |
| _version_ | 1797204986587774976 |
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| author | Pinliang Jiang Yanmei Zhang Ren Hu Xiankuan Wang Yuekun Lai Gang Rui Changjian Lin |
| author_facet | Pinliang Jiang Yanmei Zhang Ren Hu Xiankuan Wang Yuekun Lai Gang Rui Changjian Lin |
| author_sort | Pinliang Jiang |
| collection | DOAJ |
| description | Surface structures and physicochemical properties critically influence osseointegration of titanium (Ti) implants. Previous studies have shown that the surface with both micro- and nanoscale roughness may provide multiple features comparable to cell dimensions and thus efficiently regulate cell-material interaction. However, less attention has been made to further optimize the physicochemical properties (e.g., crystalline phase) and to further improve the bioactivity of micro/nanostructured surfaces. Herein, micro/nanostructured titania surfaces with different crystalline phases (amorphous, anatase and anatase/rutile) were prepared and hydroxyapatite (HA) nanorods were deposited onto the as-prepared surfaces by a spin-assisted layer-by-layer assembly method without greatly altering the initial multi-scale morphology and wettability. The effects of crystalline phase, chemical composition and wettability on osteoblast response were investigated. It is noted that all the micro/nanostructured surfaces with/without HA modification presented superamphiphilic. The activities of MC3T3-E1 cells suggested that the proliferation trend on the micro/nanostructured surfaces was greatly influenced by different crystalline phases, and the highest proliferation rate was obtained on the anatase/rutile surface, followed by the anatase; but the cell differentiation and extracellular matrix mineralization were almost the same among them. After ultrathin HA modification on the micro/nanostructured surfaces with different crystalline phases, it exhibited similar proliferation trend as the original surfaces; however, the cell differentiation and extracellular matrix mineralization were significantly improved. The results indicate that the introduction of ultrathin HA to the micro/nanostructured surfaces with optimized crystalline phase benefits cell proliferation, differentiation and maturation, which suggests a favorable biomimetic microenvironment and provides the potential for enhanced implant osseointegration in vivo. |
| first_indexed | 2024-04-24T08:43:57Z |
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| language | English |
| last_indexed | 2024-04-24T08:43:57Z |
| publishDate | 2021-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-1011bf701ffa4b08a6cde0d4b97ad5c62024-04-16T14:29:02ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2021-04-016411181129Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulationPinliang Jiang0Yanmei Zhang1Ren Hu2Xiankuan Wang3Yuekun Lai4Gang Rui5Changjian Lin6College of Materials, Xiamen University, Xiamen, 361005, China; State Key Lab of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, ChinaState Key Lab of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, ChinaState Key Lab of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, ChinaState Key Lab of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, ChinaNational Engineering Research Center of Chemical Fertilizer Catalyst (NERC-CFC), College of Chemical Engineering, Fuzhou University, Fuzhou, 350116, ChinaDepartment of Orthopedics Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, ChinaCollege of Materials, Xiamen University, Xiamen, 361005, China; State Key Lab of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China; Corresponding author. State Key Lab of Physical Chemistry of Solid Surfaces, and College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.Surface structures and physicochemical properties critically influence osseointegration of titanium (Ti) implants. Previous studies have shown that the surface with both micro- and nanoscale roughness may provide multiple features comparable to cell dimensions and thus efficiently regulate cell-material interaction. However, less attention has been made to further optimize the physicochemical properties (e.g., crystalline phase) and to further improve the bioactivity of micro/nanostructured surfaces. Herein, micro/nanostructured titania surfaces with different crystalline phases (amorphous, anatase and anatase/rutile) were prepared and hydroxyapatite (HA) nanorods were deposited onto the as-prepared surfaces by a spin-assisted layer-by-layer assembly method without greatly altering the initial multi-scale morphology and wettability. The effects of crystalline phase, chemical composition and wettability on osteoblast response were investigated. It is noted that all the micro/nanostructured surfaces with/without HA modification presented superamphiphilic. The activities of MC3T3-E1 cells suggested that the proliferation trend on the micro/nanostructured surfaces was greatly influenced by different crystalline phases, and the highest proliferation rate was obtained on the anatase/rutile surface, followed by the anatase; but the cell differentiation and extracellular matrix mineralization were almost the same among them. After ultrathin HA modification on the micro/nanostructured surfaces with different crystalline phases, it exhibited similar proliferation trend as the original surfaces; however, the cell differentiation and extracellular matrix mineralization were significantly improved. The results indicate that the introduction of ultrathin HA to the micro/nanostructured surfaces with optimized crystalline phase benefits cell proliferation, differentiation and maturation, which suggests a favorable biomimetic microenvironment and provides the potential for enhanced implant osseointegration in vivo.http://www.sciencedirect.com/science/article/pii/S2452199X20302565Micro/nanostructured surfacesCrystalline phaseHydroxyapatiteCell responseBioactivity |
| spellingShingle | Pinliang Jiang Yanmei Zhang Ren Hu Xiankuan Wang Yuekun Lai Gang Rui Changjian Lin Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation Bioactive Materials Micro/nanostructured surfaces Crystalline phase Hydroxyapatite Cell response Bioactivity |
| title | Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation |
| title_full | Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation |
| title_fullStr | Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation |
| title_full_unstemmed | Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation |
| title_short | Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation |
| title_sort | hydroxyapatite modified micro nanostructured titania surfaces with different crystalline phases for osteoblast regulation |
| topic | Micro/nanostructured surfaces Crystalline phase Hydroxyapatite Cell response Bioactivity |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X20302565 |
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