Damaged muscle fibers might masquerade as hybrid fibers – a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies
We report that, labeling mouse muscle tissue, with mouse monoclonal antibodies specific to slow or fast myosin heavy chain (sMyHC and fMyHC, respectively), can lead to artefactual labeling of damaged muscle fibers, as hybrid fibers (sMyHC+ and fMyHC+). We demonstrate that such erroneous immunopheno...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2018-07-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | https://ejh.it/index.php/ejh/article/view/2896 |
| _version_ | 1818056689112317952 |
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| author | Morium Begam Joseph A. Roche |
| author_facet | Morium Begam Joseph A. Roche |
| author_sort | Morium Begam |
| collection | DOAJ |
| description | We report that, labeling mouse muscle tissue, with mouse monoclonal antibodies specific to slow or fast myosin heavy chain (sMyHC and fMyHC, respectively), can lead to artefactual labeling of damaged muscle fibers, as hybrid fibers (sMyHC+ and fMyHC+). We demonstrate that such erroneous immunophenotyping of muscle may be avoided, by performing colabeling or serial-section-labeling, to identify damaged fibers. The quadriceps femoris muscle group (QF) in 7-month-old, male, C57BL/6J mice had: 1.21 ± 0.21%, 98.34 ± 1.06%, 0.07 ± 0.01%, and 0.53 ± 0.85% fibers, that were, sMyHC+, fMyHC+, hybrid, and damaged, respectively. All fibers in the tibialis anterior muscle (TA) of 3-month-old, male, C57BL/6J mice were fMyHC+; and at 3 days after injurious eccentric contractions, there was no fiber-type shift, but ~ 18% fibers were damaged. |
| first_indexed | 2024-12-10T12:32:50Z |
| format | Article |
| id | doaj.art-1013288cd46c40fcb51a50f5a155acf8 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-10T12:32:50Z |
| publishDate | 2018-07-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-1013288cd46c40fcb51a50f5a155acf82022-12-22T01:48:45ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062018-07-0162310.4081/ejh.2018.2896Damaged muscle fibers might masquerade as hybrid fibers – a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodiesMorium Begam0Joseph A. Roche1Wayne State University, Department of Health Care SciencesWayne State University, Department of Health Care SciencesWe report that, labeling mouse muscle tissue, with mouse monoclonal antibodies specific to slow or fast myosin heavy chain (sMyHC and fMyHC, respectively), can lead to artefactual labeling of damaged muscle fibers, as hybrid fibers (sMyHC+ and fMyHC+). We demonstrate that such erroneous immunophenotyping of muscle may be avoided, by performing colabeling or serial-section-labeling, to identify damaged fibers. The quadriceps femoris muscle group (QF) in 7-month-old, male, C57BL/6J mice had: 1.21 ± 0.21%, 98.34 ± 1.06%, 0.07 ± 0.01%, and 0.53 ± 0.85% fibers, that were, sMyHC+, fMyHC+, hybrid, and damaged, respectively. All fibers in the tibialis anterior muscle (TA) of 3-month-old, male, C57BL/6J mice were fMyHC+; and at 3 days after injurious eccentric contractions, there was no fiber-type shift, but ~ 18% fibers were damaged.https://ejh.it/index.php/ejh/article/view/2896Skeletal musclemyosin heavy chainsfiber typeimmunolabelinghybrid muscle fibersmuscle fiber damage |
| spellingShingle | Morium Begam Joseph A. Roche Damaged muscle fibers might masquerade as hybrid fibers – a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies European Journal of Histochemistry Skeletal muscle myosin heavy chains fiber type immunolabeling hybrid muscle fibers muscle fiber damage |
| title | Damaged muscle fibers might masquerade as hybrid fibers – a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies |
| title_full | Damaged muscle fibers might masquerade as hybrid fibers – a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies |
| title_fullStr | Damaged muscle fibers might masquerade as hybrid fibers – a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies |
| title_full_unstemmed | Damaged muscle fibers might masquerade as hybrid fibers – a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies |
| title_short | Damaged muscle fibers might masquerade as hybrid fibers – a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies |
| title_sort | damaged muscle fibers might masquerade as hybrid fibers a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies |
| topic | Skeletal muscle myosin heavy chains fiber type immunolabeling hybrid muscle fibers muscle fiber damage |
| url | https://ejh.it/index.php/ejh/article/view/2896 |
| work_keys_str_mv | AT moriumbegam damagedmusclefibersmightmasqueradeashybridfibersacautionarynoteonimmunophenotypingmousemusclewithmousemonoclonalantibodies AT josepharoche damagedmusclefibersmightmasqueradeashybridfibersacautionarynoteonimmunophenotypingmousemusclewithmousemonoclonalantibodies |