Ectopic Expression of <i>Perilla frutescens WRI1</i> Enhanced Storage Oil Accumulation in <i>Nicotiana benthamiana</i> Leaves
Vegetable oils are indispensable in human and animal diets and have been widely used for the production of detergents, lubricants, cosmetics, and biofuels. The seeds of an allotetraploid <i>Perilla frutescens</i> contain approximately 35 to 40% oils with high levels of polyunsaturated fa...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/5/1081 |
| _version_ | 1797614561273053184 |
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| author | Semi Kim Kyeong-Ryeol Lee Mi Chung Suh |
| author_facet | Semi Kim Kyeong-Ryeol Lee Mi Chung Suh |
| author_sort | Semi Kim |
| collection | DOAJ |
| description | Vegetable oils are indispensable in human and animal diets and have been widely used for the production of detergents, lubricants, cosmetics, and biofuels. The seeds of an allotetraploid <i>Perilla frutescens</i> contain approximately 35 to 40% oils with high levels of polyunsaturated fatty acids (PUFAs). WRINKELD1 (WRI1) encoding an AP2/ERF-type transcription factor is known to upregulate the expression of genes involved in glycolysis and fatty acid biosynthesis and TAG assembly. In this study, two <i>WRI1</i> isoforms, <i>PfWRI1A,</i> and <i>PfWRI1B</i> were isolated from Perilla and predominantly expressed in developing Perilla seeds. The fluorescent signals from <i>PfWRI1A:eYFP</i> and <i>PfWRI1B:eYFP</i> driven by the CaMV 35S promoter were detected in the nucleus of the <i>Nicotiana benthamiana</i> leaf epidermis. Ectopic expression of each of <i>PfWRI1A</i> and <i>PfWRI1B</i> increased the levels of TAG by approximately 2.9- and 2.7-fold in <i>N. benthamiana</i> leaves and particularly, the enhanced levels (mol%) of C18:2, and C18:3 in the TAGs were prominent with the concomitant reduction in the amounts of saturated fatty acids. The expression levels of <i>NbPl-PKβ1</i>, <i>NbKAS1</i>, and <i>NbFATA</i>, which were known to be target genes of <i>WRI1,</i> significantly increased in tobacco leaves overexpressing <i>PfWRI1A</i> or <i>PfWRI1B</i>. Therefore, newly characterized <i>PfWRI1A</i> and <i>PfWRI1B</i> can be potentially useful for the enhanced accumulation of storage oils with increased PUFAs in oilseed crops. |
| first_indexed | 2024-03-11T07:13:10Z |
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| language | English |
| last_indexed | 2024-03-11T07:13:10Z |
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| series | Plants |
| spelling | doaj.art-101423083875462f88e0f8321fb779cc2023-11-17T08:23:53ZengMDPI AGPlants2223-77472023-02-01125108110.3390/plants12051081Ectopic Expression of <i>Perilla frutescens WRI1</i> Enhanced Storage Oil Accumulation in <i>Nicotiana benthamiana</i> LeavesSemi Kim0Kyeong-Ryeol Lee1Mi Chung Suh2Department of Life Science, Sogang University, Seoul 04107, Republic of KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Jeonju 54875, Republic of KoreaDepartment of Life Science, Sogang University, Seoul 04107, Republic of KoreaVegetable oils are indispensable in human and animal diets and have been widely used for the production of detergents, lubricants, cosmetics, and biofuels. The seeds of an allotetraploid <i>Perilla frutescens</i> contain approximately 35 to 40% oils with high levels of polyunsaturated fatty acids (PUFAs). WRINKELD1 (WRI1) encoding an AP2/ERF-type transcription factor is known to upregulate the expression of genes involved in glycolysis and fatty acid biosynthesis and TAG assembly. In this study, two <i>WRI1</i> isoforms, <i>PfWRI1A,</i> and <i>PfWRI1B</i> were isolated from Perilla and predominantly expressed in developing Perilla seeds. The fluorescent signals from <i>PfWRI1A:eYFP</i> and <i>PfWRI1B:eYFP</i> driven by the CaMV 35S promoter were detected in the nucleus of the <i>Nicotiana benthamiana</i> leaf epidermis. Ectopic expression of each of <i>PfWRI1A</i> and <i>PfWRI1B</i> increased the levels of TAG by approximately 2.9- and 2.7-fold in <i>N. benthamiana</i> leaves and particularly, the enhanced levels (mol%) of C18:2, and C18:3 in the TAGs were prominent with the concomitant reduction in the amounts of saturated fatty acids. The expression levels of <i>NbPl-PKβ1</i>, <i>NbKAS1</i>, and <i>NbFATA</i>, which were known to be target genes of <i>WRI1,</i> significantly increased in tobacco leaves overexpressing <i>PfWRI1A</i> or <i>PfWRI1B</i>. Therefore, newly characterized <i>PfWRI1A</i> and <i>PfWRI1B</i> can be potentially useful for the enhanced accumulation of storage oils with increased PUFAs in oilseed crops.https://www.mdpi.com/2223-7747/12/5/1081<i>Perilla frutescens</i>WRINKLED1transcription factortriacylglycerolseed oil |
| spellingShingle | Semi Kim Kyeong-Ryeol Lee Mi Chung Suh Ectopic Expression of <i>Perilla frutescens WRI1</i> Enhanced Storage Oil Accumulation in <i>Nicotiana benthamiana</i> Leaves Plants <i>Perilla frutescens</i> WRINKLED1 transcription factor triacylglycerol seed oil |
| title | Ectopic Expression of <i>Perilla frutescens WRI1</i> Enhanced Storage Oil Accumulation in <i>Nicotiana benthamiana</i> Leaves |
| title_full | Ectopic Expression of <i>Perilla frutescens WRI1</i> Enhanced Storage Oil Accumulation in <i>Nicotiana benthamiana</i> Leaves |
| title_fullStr | Ectopic Expression of <i>Perilla frutescens WRI1</i> Enhanced Storage Oil Accumulation in <i>Nicotiana benthamiana</i> Leaves |
| title_full_unstemmed | Ectopic Expression of <i>Perilla frutescens WRI1</i> Enhanced Storage Oil Accumulation in <i>Nicotiana benthamiana</i> Leaves |
| title_short | Ectopic Expression of <i>Perilla frutescens WRI1</i> Enhanced Storage Oil Accumulation in <i>Nicotiana benthamiana</i> Leaves |
| title_sort | ectopic expression of i perilla frutescens wri1 i enhanced storage oil accumulation in i nicotiana benthamiana i leaves |
| topic | <i>Perilla frutescens</i> WRINKLED1 transcription factor triacylglycerol seed oil |
| url | https://www.mdpi.com/2223-7747/12/5/1081 |
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