Evaluation of the Interaction between the Poincianella pyramidalis (Tul.) LP Queiroz Extract and Antimicrobials Using Biological and Analytical Models.
Poincianella pyramidalis (Tul.) LP Queiroz (Fabaceae) is an endemic tree of northeastern Brazil, occurring mainly in the Caatinga. Its medicinal use is widespread and is an important therapeutic option against diarrhea, dysentery, and respiratory and urinary infections, among other diseases. In this...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155532&type=printable |
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| author | Thiago P Chaves Felipe Hugo A Fernandes Cleildo P Santana Jocimar S Santos Francinalva D Medeiros Délcio C Felismino Vanda L Santos Raïssa Mayer R Catão Henrique Douglas M Coutinho Ana Cláudia D Medeiros |
| author_facet | Thiago P Chaves Felipe Hugo A Fernandes Cleildo P Santana Jocimar S Santos Francinalva D Medeiros Délcio C Felismino Vanda L Santos Raïssa Mayer R Catão Henrique Douglas M Coutinho Ana Cláudia D Medeiros |
| author_sort | Thiago P Chaves |
| collection | DOAJ |
| description | Poincianella pyramidalis (Tul.) LP Queiroz (Fabaceae) is an endemic tree of northeastern Brazil, occurring mainly in the Caatinga. Its medicinal use is widespread and is an important therapeutic option against diarrhea, dysentery, and respiratory and urinary infections, among other diseases. In this study we determined the chemical marker and evaluated the interaction between P. pyramidalis extract and a commercial antimicrobial through the use of biological and analytical models. To obtain the extract, an ethanol-water mixture (50:50 v/v) was used as solvent. It was nebulized in a spray dryer using colloidal silicon dioxide as a drying adjuvant. The extract (ENPp) was subjected to HPLC analysis to verify the presence of certain secondary metabolites. The Minimum Inhibitory Concentration (MIC) of the extract against Gram-negative bacteria was determined by broth microdilution and the MIC of synthetic antimicrobial drugs in the presence and absence of the extract. The antioxidant activity of ENPp was evaluated by the DPPH method. The compatibility between the antimicrobial and the extract was evaluated by thermal analysis (TG/DTA). The acute toxicity of the extract was evaluated in vivo in rodents. The results indicate significant additive action of the extract on synthetic antibiotics, considerable antioxidant activity and absence of toxicity. This extract shows high potential for the development of formulations for antimicrobial therapy when used with a vegetable-active ingredient. |
| first_indexed | 2024-12-20T03:28:39Z |
| format | Article |
| id | doaj.art-1015723a6f2844c2b1fcf99f647d0e25 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T15:32:25Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1015723a6f2844c2b1fcf99f647d0e252025-02-25T05:34:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015553210.1371/journal.pone.0155532Evaluation of the Interaction between the Poincianella pyramidalis (Tul.) LP Queiroz Extract and Antimicrobials Using Biological and Analytical Models.Thiago P ChavesFelipe Hugo A FernandesCleildo P SantanaJocimar S SantosFrancinalva D MedeirosDélcio C FelisminoVanda L SantosRaïssa Mayer R CatãoHenrique Douglas M CoutinhoAna Cláudia D MedeirosPoincianella pyramidalis (Tul.) LP Queiroz (Fabaceae) is an endemic tree of northeastern Brazil, occurring mainly in the Caatinga. Its medicinal use is widespread and is an important therapeutic option against diarrhea, dysentery, and respiratory and urinary infections, among other diseases. In this study we determined the chemical marker and evaluated the interaction between P. pyramidalis extract and a commercial antimicrobial through the use of biological and analytical models. To obtain the extract, an ethanol-water mixture (50:50 v/v) was used as solvent. It was nebulized in a spray dryer using colloidal silicon dioxide as a drying adjuvant. The extract (ENPp) was subjected to HPLC analysis to verify the presence of certain secondary metabolites. The Minimum Inhibitory Concentration (MIC) of the extract against Gram-negative bacteria was determined by broth microdilution and the MIC of synthetic antimicrobial drugs in the presence and absence of the extract. The antioxidant activity of ENPp was evaluated by the DPPH method. The compatibility between the antimicrobial and the extract was evaluated by thermal analysis (TG/DTA). The acute toxicity of the extract was evaluated in vivo in rodents. The results indicate significant additive action of the extract on synthetic antibiotics, considerable antioxidant activity and absence of toxicity. This extract shows high potential for the development of formulations for antimicrobial therapy when used with a vegetable-active ingredient.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155532&type=printable |
| spellingShingle | Thiago P Chaves Felipe Hugo A Fernandes Cleildo P Santana Jocimar S Santos Francinalva D Medeiros Délcio C Felismino Vanda L Santos Raïssa Mayer R Catão Henrique Douglas M Coutinho Ana Cláudia D Medeiros Evaluation of the Interaction between the Poincianella pyramidalis (Tul.) LP Queiroz Extract and Antimicrobials Using Biological and Analytical Models. PLoS ONE |
| title | Evaluation of the Interaction between the Poincianella pyramidalis (Tul.) LP Queiroz Extract and Antimicrobials Using Biological and Analytical Models. |
| title_full | Evaluation of the Interaction between the Poincianella pyramidalis (Tul.) LP Queiroz Extract and Antimicrobials Using Biological and Analytical Models. |
| title_fullStr | Evaluation of the Interaction between the Poincianella pyramidalis (Tul.) LP Queiroz Extract and Antimicrobials Using Biological and Analytical Models. |
| title_full_unstemmed | Evaluation of the Interaction between the Poincianella pyramidalis (Tul.) LP Queiroz Extract and Antimicrobials Using Biological and Analytical Models. |
| title_short | Evaluation of the Interaction between the Poincianella pyramidalis (Tul.) LP Queiroz Extract and Antimicrobials Using Biological and Analytical Models. |
| title_sort | evaluation of the interaction between the poincianella pyramidalis tul lp queiroz extract and antimicrobials using biological and analytical models |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155532&type=printable |
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