Homeoviscous Adaptation of the <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress
ABSTRACT To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation. Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, an...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2021-08-01
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| Series: | mBio |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.01295-21 |
| _version_ | 1818741135106899968 |
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| author | Carmen M. Herrera Bradley J. Voss M. Stephen Trent |
| author_facet | Carmen M. Herrera Bradley J. Voss M. Stephen Trent |
| author_sort | Carmen M. Herrera |
| collection | DOAJ |
| description | ABSTRACT To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation. Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions. It is well documented, for example, that glycerophospholipid content varies substantially in both Gram-negative and Gram-positive bacteria with changes in growth temperature. However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts. LPS/LOS is anchored at the cell surface by the highly conserved lipid A domain and localized in the outer leaflet of the outer membrane. Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii. A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions. LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2′ position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity. Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier. LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms. IMPORTANCE To maintain cellular fitness, the composition of biological membranes must change in response to shifts in temperature or other stresses. This process, known as homeoviscous adaptation, allows for maintenance of optimal fluidity and membrane permeability. Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane. Although much is known regarding how glycerophospholipids are altered during temperature shifts, our understanding of LOS or LPS alterations under these conditions is lacking. Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen. |
| first_indexed | 2024-12-18T01:51:48Z |
| format | Article |
| id | doaj.art-101668c59f524abf840dfb0c763a72dd |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-12-18T01:51:48Z |
| publishDate | 2021-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-101668c59f524abf840dfb0c763a72dd2022-12-21T21:25:00ZengAmerican Society for MicrobiologymBio2150-75112021-08-0112410.1128/mBio.01295-21Homeoviscous Adaptation of the <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold StressCarmen M. Herrera0Bradley J. Voss1M. Stephen Trent2Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USADepartment of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USADepartment of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USAABSTRACT To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation. Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions. It is well documented, for example, that glycerophospholipid content varies substantially in both Gram-negative and Gram-positive bacteria with changes in growth temperature. However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts. LPS/LOS is anchored at the cell surface by the highly conserved lipid A domain and localized in the outer leaflet of the outer membrane. Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii. A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions. LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2′ position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity. Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier. LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms. IMPORTANCE To maintain cellular fitness, the composition of biological membranes must change in response to shifts in temperature or other stresses. This process, known as homeoviscous adaptation, allows for maintenance of optimal fluidity and membrane permeability. Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane. Although much is known regarding how glycerophospholipids are altered during temperature shifts, our understanding of LOS or LPS alterations under these conditions is lacking. Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen.https://journals.asm.org/doi/10.1128/mBio.01295-21Acinetobacteracylationacyltransferasecell envelopecold shocklipid A |
| spellingShingle | Carmen M. Herrera Bradley J. Voss M. Stephen Trent Homeoviscous Adaptation of the <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress mBio Acinetobacter acylation acyltransferase cell envelope cold shock lipid A |
| title | Homeoviscous Adaptation of the <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress |
| title_full | Homeoviscous Adaptation of the <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress |
| title_fullStr | Homeoviscous Adaptation of the <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress |
| title_full_unstemmed | Homeoviscous Adaptation of the <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress |
| title_short | Homeoviscous Adaptation of the <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress |
| title_sort | homeoviscous adaptation of the named content content type genus species acinetobacter baumannii named content outer membrane alteration of lipooligosaccharide structure during cold stress |
| topic | Acinetobacter acylation acyltransferase cell envelope cold shock lipid A |
| url | https://journals.asm.org/doi/10.1128/mBio.01295-21 |
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