Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation
Upon fertilization, the highly specialised sperm and oocyte genomes are remodelled to confer totipotency. The mechanisms of the dramatic reprogramming events that occur have remained unknown, and presumed roles of histone modifying enzymes are just starting to be elucidated. Here, we explore the fun...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-02-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/08851 |
| _version_ | 1811200218178256896 |
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| author | Katia Ancelin Laurène Syx Maud Borensztein Noémie Ranisavljevic Ivaylo Vassilev Luis Briseño-Roa Tao Liu Eric Metzger Nicolas Servant Emmanuel Barillot Chong-Jian Chen Roland Schüle Edith Heard |
| author_facet | Katia Ancelin Laurène Syx Maud Borensztein Noémie Ranisavljevic Ivaylo Vassilev Luis Briseño-Roa Tao Liu Eric Metzger Nicolas Servant Emmanuel Barillot Chong-Jian Chen Roland Schüle Edith Heard |
| author_sort | Katia Ancelin |
| collection | DOAJ |
| description | Upon fertilization, the highly specialised sperm and oocyte genomes are remodelled to confer totipotency. The mechanisms of the dramatic reprogramming events that occur have remained unknown, and presumed roles of histone modifying enzymes are just starting to be elucidated. Here, we explore the function of the oocyte-inherited pool of a histone H3K4 and K9 demethylase, LSD1/KDM1A during early mouse development. KDM1A deficiency results in developmental arrest by the two-cell stage, accompanied by dramatic and stepwise alterations in H3K9 and H3K4 methylation patterns. At the transcriptional level, the switch of the maternal-to-zygotic transition fails to be induced properly and LINE-1 retrotransposons are not properly silenced. We propose that KDM1A plays critical roles in establishing the correct epigenetic landscape of the zygote upon fertilization, in preserving genome integrity and in initiating new patterns of genome expression that drive early mouse development. |
| first_indexed | 2024-04-12T01:59:53Z |
| format | Article |
| id | doaj.art-101668d09c744ddfb12d6a5d6d258a70 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:59:53Z |
| publishDate | 2016-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-101668d09c744ddfb12d6a5d6d258a702022-12-22T03:52:41ZengeLife Sciences Publications LtdeLife2050-084X2016-02-01510.7554/eLife.08851Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activationKatia Ancelin0Laurène Syx1Maud Borensztein2Noémie Ranisavljevic3Ivaylo Vassilev4Luis Briseño-Roa5Tao Liu6Eric Metzger7Nicolas Servant8Emmanuel Barillot9Chong-Jian Chen10Roland Schüle11Edith Heard12https://orcid.org/0000-0001-8052-7117Institut Curie, Paris, France; Genetics and Developmental Biology Unit, INSERM, Paris, FranceInstitut Curie, Paris, France; Bioinformatics and Computational Systems Biology of Cancer, INSERM, Paris, France; Mines ParisTech, Fontainebleau, FranceInstitut Curie, Paris, France; Genetics and Developmental Biology Unit, INSERM, Paris, FranceInstitut Curie, Paris, France; Genetics and Developmental Biology Unit, INSERM, Paris, FranceInstitut Curie, Paris, France; Bioinformatics and Computational Systems Biology of Cancer, INSERM, Paris, France; Mines ParisTech, Fontainebleau, FranceHigh Fidelity Biology, Paris, FranceAnnoroad Gene Technology Co., Ltd, Beijing, ChinaUrologische Klinik und Zentrale Klinische Forschung, Freiburg, GermanyInstitut Curie, Paris, France; Bioinformatics and Computational Systems Biology of Cancer, INSERM, Paris, France; Mines ParisTech, Fontainebleau, FranceInstitut Curie, Paris, France; Bioinformatics and Computational Systems Biology of Cancer, INSERM, Paris, France; Mines ParisTech, Fontainebleau, FranceAnnoroad Gene Technology Co., Ltd, Beijing, ChinaUrologische Klinik und Zentrale Klinische Forschung, Freiburg, GermanyInstitut Curie, Paris, France; Genetics and Developmental Biology Unit, INSERM, Paris, FranceUpon fertilization, the highly specialised sperm and oocyte genomes are remodelled to confer totipotency. The mechanisms of the dramatic reprogramming events that occur have remained unknown, and presumed roles of histone modifying enzymes are just starting to be elucidated. Here, we explore the function of the oocyte-inherited pool of a histone H3K4 and K9 demethylase, LSD1/KDM1A during early mouse development. KDM1A deficiency results in developmental arrest by the two-cell stage, accompanied by dramatic and stepwise alterations in H3K9 and H3K4 methylation patterns. At the transcriptional level, the switch of the maternal-to-zygotic transition fails to be induced properly and LINE-1 retrotransposons are not properly silenced. We propose that KDM1A plays critical roles in establishing the correct epigenetic landscape of the zygote upon fertilization, in preserving genome integrity and in initiating new patterns of genome expression that drive early mouse development.https://elifesciences.org/articles/08851epigenetic reprogramminghistone demethylationpreimplantation developmentlysine methylationtranscription regulationretrotransposons |
| spellingShingle | Katia Ancelin Laurène Syx Maud Borensztein Noémie Ranisavljevic Ivaylo Vassilev Luis Briseño-Roa Tao Liu Eric Metzger Nicolas Servant Emmanuel Barillot Chong-Jian Chen Roland Schüle Edith Heard Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation eLife epigenetic reprogramming histone demethylation preimplantation development lysine methylation transcription regulation retrotransposons |
| title | Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation |
| title_full | Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation |
| title_fullStr | Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation |
| title_full_unstemmed | Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation |
| title_short | Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation |
| title_sort | maternal lsd1 kdm1a is an essential regulator of chromatin and transcription landscapes during zygotic genome activation |
| topic | epigenetic reprogramming histone demethylation preimplantation development lysine methylation transcription regulation retrotransposons |
| url | https://elifesciences.org/articles/08851 |
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