Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages.
Hepatic ischemia-reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sha...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4747576?pdf=render |
| _version_ | 1818036634121142272 |
|---|---|
| author | Weisheng Zheng Hewei Men Jing Li Yu Xing Bin Wu Zhenglu Wang Junjie Li Dahong Teng Yuan Shi Jiang Li Pu Jiang Jinzhen Cai |
| author_facet | Weisheng Zheng Hewei Men Jing Li Yu Xing Bin Wu Zhenglu Wang Junjie Li Dahong Teng Yuan Shi Jiang Li Pu Jiang Jinzhen Cai |
| author_sort | Weisheng Zheng |
| collection | DOAJ |
| description | Hepatic ischemia-reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sham control, ischemia stage, and reperfusion stage. We generated global expression profiles of microRNA and mRNA genes in mouse livers subjected to ischemia-reperfusion injury at the three stages, respectively. Comparison analysis showed that reperfusion injury had a distinct expression profile whereas the ischemia sample and the sham control were clustered together. Consistently, there are 69 differentially expressed microRNAs between the reperfusion sample and the sham control whereas 28 differentially expressed microRNAs between the ischemia sample and the sham control. We further identified two modes of microRNA expression changes in ischemia-reperfusion injury. Functional analysis of both the differentially expressed microRNAs in the two modes and their target mRNAs revealed that ischemia injury impaired mitochondrial function, nutrient consumption, and metabolism process. In contrast, reperfusion injury led to severe tissue inflammation that is predominantly an innate-immune response in the ischemia-reperfusion process. Our staged analysis of gene expression profiles provides new insights into regulatory mechanisms of microRNAs in mouse hepatic IR injury. |
| first_indexed | 2024-12-10T07:14:04Z |
| format | Article |
| id | doaj.art-1017fd12a7ad4258b78d18f4665b7d23 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T07:14:04Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1017fd12a7ad4258b78d18f4665b7d232022-12-22T01:57:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01112e014867710.1371/journal.pone.0148677Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages.Weisheng ZhengHewei MenJing LiYu XingBin WuZhenglu WangJunjie LiDahong TengYuan ShiJiang LiPu JiangJinzhen CaiHepatic ischemia-reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sham control, ischemia stage, and reperfusion stage. We generated global expression profiles of microRNA and mRNA genes in mouse livers subjected to ischemia-reperfusion injury at the three stages, respectively. Comparison analysis showed that reperfusion injury had a distinct expression profile whereas the ischemia sample and the sham control were clustered together. Consistently, there are 69 differentially expressed microRNAs between the reperfusion sample and the sham control whereas 28 differentially expressed microRNAs between the ischemia sample and the sham control. We further identified two modes of microRNA expression changes in ischemia-reperfusion injury. Functional analysis of both the differentially expressed microRNAs in the two modes and their target mRNAs revealed that ischemia injury impaired mitochondrial function, nutrient consumption, and metabolism process. In contrast, reperfusion injury led to severe tissue inflammation that is predominantly an innate-immune response in the ischemia-reperfusion process. Our staged analysis of gene expression profiles provides new insights into regulatory mechanisms of microRNAs in mouse hepatic IR injury.http://europepmc.org/articles/PMC4747576?pdf=render |
| spellingShingle | Weisheng Zheng Hewei Men Jing Li Yu Xing Bin Wu Zhenglu Wang Junjie Li Dahong Teng Yuan Shi Jiang Li Pu Jiang Jinzhen Cai Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages. PLoS ONE |
| title | Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages. |
| title_full | Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages. |
| title_fullStr | Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages. |
| title_full_unstemmed | Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages. |
| title_short | Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages. |
| title_sort | global microrna expression profiling of mouse livers following ischemia reperfusion injury at different stages |
| url | http://europepmc.org/articles/PMC4747576?pdf=render |
| work_keys_str_mv | AT weishengzheng globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT heweimen globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT jingli globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT yuxing globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT binwu globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT zhengluwang globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT junjieli globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT dahongteng globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT yuanshi globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT jiangli globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT pujiang globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages AT jinzhencai globalmicrornaexpressionprofilingofmouseliversfollowingischemiareperfusioninjuryatdifferentstages |