Hematopoietic Stem Cell and Fibroblast Proliferation Following Platelet Electrostimulation
The increasing use of activated platelet rich plasma (PRP), or platelet gel, for wound healing has highlighted the disadvantages of using bovine thrombin (BT), an animal derived platelet activator, including high cost and antibody stimulation. This has motivated the exploration of direct (conductive...
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| Format: | Article |
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IEEE
2018-01-01
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| Series: | IEEE Access |
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| Online Access: | https://ieeexplore.ieee.org/document/8478267/ |
| _version_ | 1818876808159821824 |
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| author | Jason Castle Allen L. Garner Reginald D. Smith Brian M. Davis Steve Klopman Sean R. Dinn Andrew S. Torres V. Bogdan Neculaes |
| author_facet | Jason Castle Allen L. Garner Reginald D. Smith Brian M. Davis Steve Klopman Sean R. Dinn Andrew S. Torres V. Bogdan Neculaes |
| author_sort | Jason Castle |
| collection | DOAJ |
| description | The increasing use of activated platelet rich plasma (PRP), or platelet gel, for wound healing has highlighted the disadvantages of using bovine thrombin (BT), an animal derived platelet activator, including high cost and antibody stimulation. This has motivated the exploration of direct (conductive coupling) and indirect (capacitive coupling) electrostimulation for <i>ex vivo</i> PRP activation to similar levels as BT in terms of growth factor release. PRP is a complex biological matrix comprising other blood cell types besides platelets. This paper assesses the impact of electrostimulation on other blood cells, specifically hematopoietic stem cells and fibroblasts. Capacitive coupling induces similar levels of cell viability and proliferation as BT 14 days following electrostimulation, while conductive coupling induces lower viability and proliferation. This indicates the potential tunability of electrostimulation to achieve equivalent efficacy as BT without the associated disadvantages and motivates future experiments to assess the implications on <i>in vivo</i> wound healing. |
| first_indexed | 2024-12-19T13:48:16Z |
| format | Article |
| id | doaj.art-101ea7fe452e4a4ea3856924ddeb6820 |
| institution | Directory Open Access Journal |
| issn | 2169-3536 |
| language | English |
| last_indexed | 2024-12-19T13:48:16Z |
| publishDate | 2018-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Access |
| spelling | doaj.art-101ea7fe452e4a4ea3856924ddeb68202022-12-21T20:18:49ZengIEEEIEEE Access2169-35362018-01-016563955640110.1109/ACCESS.2018.28729308478267Hematopoietic Stem Cell and Fibroblast Proliferation Following Platelet ElectrostimulationJason Castle0Allen L. Garner1https://orcid.org/0000-0001-5416-7437Reginald D. Smith2Brian M. Davis3Steve Klopman4Sean R. Dinn5Andrew S. Torres6V. Bogdan Neculaes7GE Global Research Center, Niskayuna, NY, USASchool of Nuclear Engineering, Purdue University, West Lafayette, IN, USAGE Global Research Center, Niskayuna, NY, USAGE Global Research Center, Niskayuna, NY, USAGE Global Research Center, Niskayuna, NY, USAGE Global Research Center, Niskayuna, NY, USAGE Global Research Center, Niskayuna, NY, USAGE Global Research Center, Niskayuna, NY, USAThe increasing use of activated platelet rich plasma (PRP), or platelet gel, for wound healing has highlighted the disadvantages of using bovine thrombin (BT), an animal derived platelet activator, including high cost and antibody stimulation. This has motivated the exploration of direct (conductive coupling) and indirect (capacitive coupling) electrostimulation for <i>ex vivo</i> PRP activation to similar levels as BT in terms of growth factor release. PRP is a complex biological matrix comprising other blood cell types besides platelets. This paper assesses the impact of electrostimulation on other blood cells, specifically hematopoietic stem cells and fibroblasts. Capacitive coupling induces similar levels of cell viability and proliferation as BT 14 days following electrostimulation, while conductive coupling induces lower viability and proliferation. This indicates the potential tunability of electrostimulation to achieve equivalent efficacy as BT without the associated disadvantages and motivates future experiments to assess the implications on <i>in vivo</i> wound healing.https://ieeexplore.ieee.org/document/8478267/Bioelectric phenomenastem cellsbiological cellsplatelet activationelectroporation |
| spellingShingle | Jason Castle Allen L. Garner Reginald D. Smith Brian M. Davis Steve Klopman Sean R. Dinn Andrew S. Torres V. Bogdan Neculaes Hematopoietic Stem Cell and Fibroblast Proliferation Following Platelet Electrostimulation IEEE Access Bioelectric phenomena stem cells biological cells platelet activation electroporation |
| title | Hematopoietic Stem Cell and Fibroblast Proliferation Following Platelet Electrostimulation |
| title_full | Hematopoietic Stem Cell and Fibroblast Proliferation Following Platelet Electrostimulation |
| title_fullStr | Hematopoietic Stem Cell and Fibroblast Proliferation Following Platelet Electrostimulation |
| title_full_unstemmed | Hematopoietic Stem Cell and Fibroblast Proliferation Following Platelet Electrostimulation |
| title_short | Hematopoietic Stem Cell and Fibroblast Proliferation Following Platelet Electrostimulation |
| title_sort | hematopoietic stem cell and fibroblast proliferation following platelet electrostimulation |
| topic | Bioelectric phenomena stem cells biological cells platelet activation electroporation |
| url | https://ieeexplore.ieee.org/document/8478267/ |
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