| Summary: | The whole chloroplast (cp) genome sequence of Populus nigra L. has been characterized from Illumina pair-end sequencing. The complete cp genome was 156,451 bp in length, containing a large single copy region (LSC) of 84,529 bp, and a small single copy region (SSC) of 16,580 bp, which were separated by a pair of 27,671 bp inverted repeat regions (IRs). The genome contained 144 genes, including 99 protein-coding genes (84 PCG species), 37 tRNA genes (30 tRNA species), and 8 ribosomal RNA genes (4 rRNA species). Most of the gene species occur as a single copy, while 26 gene species occur in double copies. The overall AT content of P. nigra cp genome is 63.2%, while the corresponding values of the LSC, SSC, and IR regions are 65.4, 69.5, and 58.0%, respectively. Further, phylogenetic analysis suggested that P. nigra is closely related to the species of P. alba.
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