The circadian timing of noise exposure influences noise-induced inflammatory responses in the mouse cochlea
Introduction: Noise-induced hearing loss is one of the most common forms of sensorineural hearing loss. Nevertheless, the mechanisms of noise-induced hearing loss are still not fully understood. Objective: To investigate the dynamics of inflammatory responses in the mammalian cochlea following noise...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Brazilian Journal of Otorhinolaryngology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1808869421001063 |
| _version_ | 1811207021737803776 |
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| author | Shichang Li Hongwei Zheng Zhimin Xing Yan Liu Lin Han Zijing Wang Lisheng Yu |
| author_facet | Shichang Li Hongwei Zheng Zhimin Xing Yan Liu Lin Han Zijing Wang Lisheng Yu |
| author_sort | Shichang Li |
| collection | DOAJ |
| description | Introduction: Noise-induced hearing loss is one of the most common forms of sensorineural hearing loss. Nevertheless, the mechanisms of noise-induced hearing loss are still not fully understood. Objective: To investigate the dynamics of inflammatory responses in the mammalian cochlea following noise trauma at two different times, once during the light cycle and once during the dark. Methods: We challenged C57BL/6J mice with moderate, continuous noise trauma at either 9 a.m. or 9 p.m. Auditory function, histological changes in hair cells, and modifications in gene expression levels of inflammatory mediators were assessed at specific time points. Shifts in auditory brainstem response thresholds were measured at 1, 3, 7 and 14 days after noise exposure to measure potential noise-induced hearing loss. Cochlear basilar-membrane immunofluorescent staining was performed at 3 and 14 days after noise exposure. The mRNA levels of several inflammatory mediators were measured via quantitative real-time polymerase chain reaction before (pre) and after (0, 3, 12, 24 and 72 h) noise exposure. Results: We found that all noise-exposed mice developed a temporary threshold shift and that there were no significant differences between daytime and nighttime noise exposures in terms of inducing hearing-threshold shifts. Similarly, we did not detect significant histological changes in hair cells between these two groups. However, we discovered an interesting phenomenon in that the peak mRNA levels of IL-1β, IL-6, CCL2 and TNF-α were higher in day noise-exposed mice compared to those in night noise-exposed mice, and these mRNA levels subsided more slowly in day noise-exposed mice. Conclusion: Overall, these observations suggest that the circadian timing of noise exposure has a significant effect on noise-induced inflammatory responses in the mouse cochlea and that a greater inflammatory response might occur after daytime exposure. |
| first_indexed | 2024-04-12T03:56:50Z |
| format | Article |
| id | doaj.art-101efaa77cf54db38e3e78bd18b364b2 |
| institution | Directory Open Access Journal |
| issn | 1808-8694 |
| language | English |
| last_indexed | 2024-04-12T03:56:50Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Otorhinolaryngology |
| spelling | doaj.art-101efaa77cf54db38e3e78bd18b364b22022-12-22T03:48:48ZengElsevierBrazilian Journal of Otorhinolaryngology1808-86942022-11-0188S1S8The circadian timing of noise exposure influences noise-induced inflammatory responses in the mouse cochleaShichang Li0Hongwei Zheng1Zhimin Xing2Yan Liu3Lin Han4Zijing Wang5Lisheng Yu6Peking University People's Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Beijing, PR ChinaPeking University People's Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Beijing, PR ChinaPeking University People's Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Beijing, PR ChinaPeking University People's Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Beijing, PR ChinaPeking University People's Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Beijing, PR ChinaBei Jing Ji Shui Tan Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Beijing, PR ChinaPeking University People's Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Beijing, PR China; Corresponding author.Introduction: Noise-induced hearing loss is one of the most common forms of sensorineural hearing loss. Nevertheless, the mechanisms of noise-induced hearing loss are still not fully understood. Objective: To investigate the dynamics of inflammatory responses in the mammalian cochlea following noise trauma at two different times, once during the light cycle and once during the dark. Methods: We challenged C57BL/6J mice with moderate, continuous noise trauma at either 9 a.m. or 9 p.m. Auditory function, histological changes in hair cells, and modifications in gene expression levels of inflammatory mediators were assessed at specific time points. Shifts in auditory brainstem response thresholds were measured at 1, 3, 7 and 14 days after noise exposure to measure potential noise-induced hearing loss. Cochlear basilar-membrane immunofluorescent staining was performed at 3 and 14 days after noise exposure. The mRNA levels of several inflammatory mediators were measured via quantitative real-time polymerase chain reaction before (pre) and after (0, 3, 12, 24 and 72 h) noise exposure. Results: We found that all noise-exposed mice developed a temporary threshold shift and that there were no significant differences between daytime and nighttime noise exposures in terms of inducing hearing-threshold shifts. Similarly, we did not detect significant histological changes in hair cells between these two groups. However, we discovered an interesting phenomenon in that the peak mRNA levels of IL-1β, IL-6, CCL2 and TNF-α were higher in day noise-exposed mice compared to those in night noise-exposed mice, and these mRNA levels subsided more slowly in day noise-exposed mice. Conclusion: Overall, these observations suggest that the circadian timing of noise exposure has a significant effect on noise-induced inflammatory responses in the mouse cochlea and that a greater inflammatory response might occur after daytime exposure.http://www.sciencedirect.com/science/article/pii/S1808869421001063Noise-induced hearing lossCircadian timingCochleaInflammation |
| spellingShingle | Shichang Li Hongwei Zheng Zhimin Xing Yan Liu Lin Han Zijing Wang Lisheng Yu The circadian timing of noise exposure influences noise-induced inflammatory responses in the mouse cochlea Brazilian Journal of Otorhinolaryngology Noise-induced hearing loss Circadian timing Cochlea Inflammation |
| title | The circadian timing of noise exposure influences noise-induced inflammatory responses in the mouse cochlea |
| title_full | The circadian timing of noise exposure influences noise-induced inflammatory responses in the mouse cochlea |
| title_fullStr | The circadian timing of noise exposure influences noise-induced inflammatory responses in the mouse cochlea |
| title_full_unstemmed | The circadian timing of noise exposure influences noise-induced inflammatory responses in the mouse cochlea |
| title_short | The circadian timing of noise exposure influences noise-induced inflammatory responses in the mouse cochlea |
| title_sort | circadian timing of noise exposure influences noise induced inflammatory responses in the mouse cochlea |
| topic | Noise-induced hearing loss Circadian timing Cochlea Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S1808869421001063 |
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