The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution.
Cell therapy is a promising treatment. One of the key aspects of cell processing products is ensuring sterility of cell-processing facilities (CPFs). The objective of this study was to assess the environmental risk factors inside and outside CPFs. We monitored the temperature, humidity, particle num...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0236600 |
| _version_ | 1819126054894174208 |
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| author | Mitsuru Mizuno Kentaro Endo Hisako Katano Ayako Tsuji Naomi Kojima Ken Watanabe Norio Shimizu Tomohiro Morio Ichiro Sekiya |
| author_facet | Mitsuru Mizuno Kentaro Endo Hisako Katano Ayako Tsuji Naomi Kojima Ken Watanabe Norio Shimizu Tomohiro Morio Ichiro Sekiya |
| author_sort | Mitsuru Mizuno |
| collection | DOAJ |
| description | Cell therapy is a promising treatment. One of the key aspects of cell processing products is ensuring sterility of cell-processing facilities (CPFs). The objective of this study was to assess the environmental risk factors inside and outside CPFs. We monitored the temperature, humidity, particle number, colony number of microorganisms, bacteria, fungi, and harmful insects in and around our CPF monthly over one year. The temperature in the CPF was constant but the humidity fluctuated depending on the humidity outside. The particle number correlated with the number of entries to the room. Except for winter, colonies of microorganisms and harmful insects were detected depending on the cleanliness of the room. Seven bacterial and two fungal species were identified by PCR analyses. Psocoptera and Acari each accounted for 41% of the total trapped insects. These results provide useful data for taking the appropriate steps to keep entire CPFs clean. |
| first_indexed | 2024-12-22T07:49:56Z |
| format | Article |
| id | doaj.art-1028daf0cf3245ebbb4542d6f7c38edc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T07:49:56Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1028daf0cf3245ebbb4542d6f7c38edc2022-12-21T18:33:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01158e023660010.1371/journal.pone.0236600The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution.Mitsuru MizunoKentaro EndoHisako KatanoAyako TsujiNaomi KojimaKen WatanabeNorio ShimizuTomohiro MorioIchiro SekiyaCell therapy is a promising treatment. One of the key aspects of cell processing products is ensuring sterility of cell-processing facilities (CPFs). The objective of this study was to assess the environmental risk factors inside and outside CPFs. We monitored the temperature, humidity, particle number, colony number of microorganisms, bacteria, fungi, and harmful insects in and around our CPF monthly over one year. The temperature in the CPF was constant but the humidity fluctuated depending on the humidity outside. The particle number correlated with the number of entries to the room. Except for winter, colonies of microorganisms and harmful insects were detected depending on the cleanliness of the room. Seven bacterial and two fungal species were identified by PCR analyses. Psocoptera and Acari each accounted for 41% of the total trapped insects. These results provide useful data for taking the appropriate steps to keep entire CPFs clean.https://doi.org/10.1371/journal.pone.0236600 |
| spellingShingle | Mitsuru Mizuno Kentaro Endo Hisako Katano Ayako Tsuji Naomi Kojima Ken Watanabe Norio Shimizu Tomohiro Morio Ichiro Sekiya The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution. PLoS ONE |
| title | The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution. |
| title_full | The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution. |
| title_fullStr | The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution. |
| title_full_unstemmed | The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution. |
| title_short | The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution. |
| title_sort | environmental risk assessment of cell processing facilities for cell therapy in a japanese academic institution |
| url | https://doi.org/10.1371/journal.pone.0236600 |
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