A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>
Ubiquitin ligase (E3) plays a versatile role in gonadal development and spermatogenesis in mammals, while its function in fish is little reported. In this study, a Z-chromosome linked ubiquitin ligase <i>rchy1</i> in <i>C. semilaevis</i> (<i>Cs-rchy1</i>) was clon...
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MDPI AG
2021-11-01
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| author | Yuxuan Sun Ying Zhu Peng Cheng Mengqian Zhang Na Wang Zhongkai Cui Min Wei Wenteng Xu |
| author_facet | Yuxuan Sun Ying Zhu Peng Cheng Mengqian Zhang Na Wang Zhongkai Cui Min Wei Wenteng Xu |
| author_sort | Yuxuan Sun |
| collection | DOAJ |
| description | Ubiquitin ligase (E3) plays a versatile role in gonadal development and spermatogenesis in mammals, while its function in fish is little reported. In this study, a Z-chromosome linked ubiquitin ligase <i>rchy1</i> in <i>C. semilaevis</i> (<i>Cs-rchy1</i>) was cloned and characterized. The full-length cDNA was composed of 1962 bp, including 551 bp 5′UTR, 736 bp 3′UTR, and 675 bp ORF encoding a 224-amino-acid (aa) protein. <i>Cs-rchy1</i> was examined among seven different tissues and found to be predominantly expressed in gonads. In testis, <i>Cs-rchy1</i> could be detected from 40 days post hatching (dph) until 3 years post hatching (yph), but there was a significant increase at 6 months post hatching (mph). In comparison, the expression levels in ovary were rather stable among different developmental stages. In situ hybridization showed that <i>Cs-rchy1</i> was mainly localized in germ cells, that is, spermatid and spermatozoa in testis and stage I, II and III oocytes in ovary. In vitro RNA interference found that <i>Cs-rchy1</i> knockdown resulted in the decline of <i>sox9</i> and <i>igf1</i> in ovarian cell line and down-regulation of <i>cyp19a</i> in the testicular cell line. These data suggested that <i>Cs-rchy1</i> might participate in gonadal differentiation and gametogenesis, via regulating steroid hormone synthesis. |
| first_indexed | 2024-03-10T05:45:46Z |
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| last_indexed | 2024-03-10T05:45:46Z |
| publishDate | 2021-11-01 |
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| series | Animals |
| spelling | doaj.art-1029e226c79d41a6936adca09820784e2023-11-22T22:08:00ZengMDPI AGAnimals2076-26152021-11-011111326510.3390/ani11113265A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>Yuxuan Sun0Ying Zhu1Peng Cheng2Mengqian Zhang3Na Wang4Zhongkai Cui5Min Wei6Wenteng Xu7Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, ChinaJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, ChinaUbiquitin ligase (E3) plays a versatile role in gonadal development and spermatogenesis in mammals, while its function in fish is little reported. In this study, a Z-chromosome linked ubiquitin ligase <i>rchy1</i> in <i>C. semilaevis</i> (<i>Cs-rchy1</i>) was cloned and characterized. The full-length cDNA was composed of 1962 bp, including 551 bp 5′UTR, 736 bp 3′UTR, and 675 bp ORF encoding a 224-amino-acid (aa) protein. <i>Cs-rchy1</i> was examined among seven different tissues and found to be predominantly expressed in gonads. In testis, <i>Cs-rchy1</i> could be detected from 40 days post hatching (dph) until 3 years post hatching (yph), but there was a significant increase at 6 months post hatching (mph). In comparison, the expression levels in ovary were rather stable among different developmental stages. In situ hybridization showed that <i>Cs-rchy1</i> was mainly localized in germ cells, that is, spermatid and spermatozoa in testis and stage I, II and III oocytes in ovary. In vitro RNA interference found that <i>Cs-rchy1</i> knockdown resulted in the decline of <i>sox9</i> and <i>igf1</i> in ovarian cell line and down-regulation of <i>cyp19a</i> in the testicular cell line. These data suggested that <i>Cs-rchy1</i> might participate in gonadal differentiation and gametogenesis, via regulating steroid hormone synthesis.https://www.mdpi.com/2076-2615/11/11/3265<i>Cynoglossus semilaevis</i><i>Cs-rchy1</i>ubiquitin ligasesexpression patternRNA interference |
| spellingShingle | Yuxuan Sun Ying Zhu Peng Cheng Mengqian Zhang Na Wang Zhongkai Cui Min Wei Wenteng Xu A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i> Animals <i>Cynoglossus semilaevis</i> <i>Cs-rchy1</i> ubiquitin ligases expression pattern RNA interference |
| title | A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i> |
| title_full | A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i> |
| title_fullStr | A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i> |
| title_full_unstemmed | A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i> |
| title_short | A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i> |
| title_sort | z linked e3 ubiquitin ligase i cs rchy1 i is involved in gametogenesis in chinese tongue sole i cynoglossus semilaevis i |
| topic | <i>Cynoglossus semilaevis</i> <i>Cs-rchy1</i> ubiquitin ligases expression pattern RNA interference |
| url | https://www.mdpi.com/2076-2615/11/11/3265 |
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