The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy

Optical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observation of delicate biological samples. The meaning...

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Main Author: Colin J. R. Sheppard
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/11/19/8981
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author Colin J. R. Sheppard
author_facet Colin J. R. Sheppard
author_sort Colin J. R. Sheppard
collection DOAJ
description Optical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observation of delicate biological samples. The meaning of resolution and the basic principles and different approaches to superresolution are discussed.
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spelling doaj.art-102c3cffccff4a4c976de2e70347ac312023-11-22T15:45:58ZengMDPI AGApplied Sciences2076-34172021-09-011119898110.3390/app11198981The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning MicroscopyColin J. R. Sheppard0Nanophysics Istituto Italiano di Tecnologia, Via Enrico Melen, 83 Edificio B, 16152 Genova, ItalyOptical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observation of delicate biological samples. The meaning of resolution and the basic principles and different approaches to superresolution are discussed.https://www.mdpi.com/2076-3417/11/19/8981super-resolutionconfocal microscopyimage scanning microscopynonlinear microscopydeconvolution
spellingShingle Colin J. R. Sheppard
The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
Applied Sciences
super-resolution
confocal microscopy
image scanning microscopy
nonlinear microscopy
deconvolution
title The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
title_full The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
title_fullStr The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
title_full_unstemmed The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
title_short The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
title_sort development of microscopy for super resolution confocal microscopy and image scanning microscopy
topic super-resolution
confocal microscopy
image scanning microscopy
nonlinear microscopy
deconvolution
url https://www.mdpi.com/2076-3417/11/19/8981
work_keys_str_mv AT colinjrsheppard thedevelopmentofmicroscopyforsuperresolutionconfocalmicroscopyandimagescanningmicroscopy
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