Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa
Honey has a complex chemistry, and its broad-spectrum antimicrobial activity varies with floral source, climate, and harvesting conditions. Methylglyoxal was identified as the dominant antibacterial component of manuka honey. Although it has been known that methylglyoxal has antibacterial activity a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-04-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00180/full |
| _version_ | 1819118472730247168 |
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| author | Katsuhiko eHayashi Aiko eFukushima Mitsuko eHasyashi-Nishino Kunihiko eNishino |
| author_facet | Katsuhiko eHayashi Aiko eFukushima Mitsuko eHasyashi-Nishino Kunihiko eNishino |
| author_sort | Katsuhiko eHayashi |
| collection | DOAJ |
| description | Honey has a complex chemistry, and its broad-spectrum antimicrobial activity varies with floral source, climate, and harvesting conditions. Methylglyoxal was identified as the dominant antibacterial component of manuka honey. Although it has been known that methylglyoxal has antibacterial activity against gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus, there is not much information describing its activity against gram-negative bacteria. In this study, we report the effect of methylglyoxal against multidrug-resistant Pseudomonas aeruginosa (MDRP) using 53 clinically isolated strains. We also assessed the effect of deleting the five multidrug efflux systems in P. aeruginosa, as well as the efflux systems in Escherichia coli and Salmonella enterica serovar Typhimurium, on MICs of methylglyoxal. Our results indicate that methylglyoxal inhibits the growth of MDRP at concentrations of 128–512 µg/ml (1.7–7.1 mM) and is not recognized by drug efflux systems. |
| first_indexed | 2024-12-22T05:49:25Z |
| format | Article |
| id | doaj.art-102ca14ce39c4d088e3a944a5ccc529e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T05:49:25Z |
| publishDate | 2014-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-102ca14ce39c4d088e3a944a5ccc529e2022-12-21T18:36:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2014-04-01510.3389/fmicb.2014.0018053078Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosaKatsuhiko eHayashi0Aiko eFukushima1Mitsuko eHasyashi-Nishino2Kunihiko eNishino3Institute of Scientific and Industrial Research, Osaka UniversityInstitute of Scientific and Industrial Research, Osaka UniversityInstitute of Scientific and Industrial Research, Osaka UniversityInstitute of Scientific and Industrial Research, Osaka UniversityHoney has a complex chemistry, and its broad-spectrum antimicrobial activity varies with floral source, climate, and harvesting conditions. Methylglyoxal was identified as the dominant antibacterial component of manuka honey. Although it has been known that methylglyoxal has antibacterial activity against gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus, there is not much information describing its activity against gram-negative bacteria. In this study, we report the effect of methylglyoxal against multidrug-resistant Pseudomonas aeruginosa (MDRP) using 53 clinically isolated strains. We also assessed the effect of deleting the five multidrug efflux systems in P. aeruginosa, as well as the efflux systems in Escherichia coli and Salmonella enterica serovar Typhimurium, on MICs of methylglyoxal. Our results indicate that methylglyoxal inhibits the growth of MDRP at concentrations of 128–512 µg/ml (1.7–7.1 mM) and is not recognized by drug efflux systems.http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00180/fullPseudomonas aeruginosamultidrug resistancedrug efflux systemmethylglyoxalManuka honey |
| spellingShingle | Katsuhiko eHayashi Aiko eFukushima Mitsuko eHasyashi-Nishino Kunihiko eNishino Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa Frontiers in Microbiology Pseudomonas aeruginosa multidrug resistance drug efflux system methylglyoxal Manuka honey |
| title | Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa |
| title_full | Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa |
| title_fullStr | Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa |
| title_full_unstemmed | Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa |
| title_short | Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa |
| title_sort | effect of methylglyoxal on multidrug resistant pseudomonas aeruginosa |
| topic | Pseudomonas aeruginosa multidrug resistance drug efflux system methylglyoxal Manuka honey |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00180/full |
| work_keys_str_mv | AT katsuhikoehayashi effectofmethylglyoxalonmultidrugresistantpseudomonasaeruginosa AT aikoefukushima effectofmethylglyoxalonmultidrugresistantpseudomonasaeruginosa AT mitsukoehasyashinishino effectofmethylglyoxalonmultidrugresistantpseudomonasaeruginosa AT kunihikoenishino effectofmethylglyoxalonmultidrugresistantpseudomonasaeruginosa |