DNA-barcoding and Species Identification for Some Saudi Arabia Seaweeds using rbcL Gene
Among the different biological sources, seaweeds have lot of biotechnological applications. Saudi Arabia is bounded by three bodies of water. With a coastal border of almost 1,800 km. This area high species richness caused by its complex geological history has encompassed genetic and morphological d...
| 主要な著者: | , , , , , , |
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| フォーマット: | 論文 |
| 言語: | English |
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Journal of Pure and Applied Microbiology
2019-12-01
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| シリーズ: | Journal of Pure and Applied Microbiology |
| 主題: | |
| オンライン･アクセス: | https://microbiologyjournal.org/dna-barcoding-and-species-identification-for-some-saudi-arabia-seaweeds-using-rbcl-gene/ |
| _version_ | 1831601522536873984 |
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| author | Mohammed Ali Alshehri Al Thabiani Aziz Othman Alzahrani Abdulrahman Alasmari Shafik Ibrahim Gamal Osman Omar Bahattab |
| author_facet | Mohammed Ali Alshehri Al Thabiani Aziz Othman Alzahrani Abdulrahman Alasmari Shafik Ibrahim Gamal Osman Omar Bahattab |
| author_sort | Mohammed Ali Alshehri |
| collection | DOAJ |
| description | Among the different biological sources, seaweeds have lot of biotechnological applications. Saudi Arabia is bounded by three bodies of water. With a coastal border of almost 1,800 km. This area high species richness caused by its complex geological history has encompassed genetic and morphological diversity studies for decades. The DNA-barcoding using rbcL gene has proved its usefulness in studying seaweeds phylogenetic diversity, multiple cryptic introductions, environmental modulation the geographical distribution and species identification in different seaweed species. Eight algae samples were collected from different locations in Saudi Arabia. The rbcL gene was used through PCR protocol for species identification. A total number of 8 sequences were obtained with a total sequence length of 5263 bp. where it ranged from 610 to 753 with an average length of 658 bp. The species identification revealed that the specimens samples 1,2,3,4,5,6,7 and 8 belongs to Padina pavonica, Turbinaria gracilis, Carpomitra costata, Pterocladiella capillacea, Cladostephus spongiosus, Ulva lactuca, Sporochnus comosus and Sargassum muticum respectively. The rbcL-based DNA bar-coding was almost successful to identify different seaweeds specimens according to species and genus. Some specimens rbcL was not adequate to identify genus level and failed to differentiate between highly similar species. We suggest to use more DNA barcoding techniques in addition to rbcL to ad more resolution to the species identification. |
| first_indexed | 2024-12-18T15:03:59Z |
| format | Article |
| id | doaj.art-102de2c9ae59461e8fe95c0f98756a9f |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-18T15:03:59Z |
| publishDate | 2019-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-102de2c9ae59461e8fe95c0f98756a9f2022-12-21T21:03:50ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2019-12-011342035204410.22207/JPAM.13.4.15DNA-barcoding and Species Identification for Some Saudi Arabia Seaweeds using rbcL GeneMohammed Ali Alshehri0Al Thabiani Aziz1Othman Alzahrani2Abdulrahman Alasmari3Shafik Ibrahim4Gamal Osman5Omar Bahattab61Biology Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia. 2Genome and Biotechnology Unit, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia.1Biology Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia. 1Biology Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia. 2Genome and Biotechnology Unit, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia.1Biology Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia. 3Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt.3Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt. 4Department of Biology, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, Saudi Arabia.1Biology Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia. Among the different biological sources, seaweeds have lot of biotechnological applications. Saudi Arabia is bounded by three bodies of water. With a coastal border of almost 1,800 km. This area high species richness caused by its complex geological history has encompassed genetic and morphological diversity studies for decades. The DNA-barcoding using rbcL gene has proved its usefulness in studying seaweeds phylogenetic diversity, multiple cryptic introductions, environmental modulation the geographical distribution and species identification in different seaweed species. Eight algae samples were collected from different locations in Saudi Arabia. The rbcL gene was used through PCR protocol for species identification. A total number of 8 sequences were obtained with a total sequence length of 5263 bp. where it ranged from 610 to 753 with an average length of 658 bp. The species identification revealed that the specimens samples 1,2,3,4,5,6,7 and 8 belongs to Padina pavonica, Turbinaria gracilis, Carpomitra costata, Pterocladiella capillacea, Cladostephus spongiosus, Ulva lactuca, Sporochnus comosus and Sargassum muticum respectively. The rbcL-based DNA bar-coding was almost successful to identify different seaweeds specimens according to species and genus. Some specimens rbcL was not adequate to identify genus level and failed to differentiate between highly similar species. We suggest to use more DNA barcoding techniques in addition to rbcL to ad more resolution to the species identification.https://microbiologyjournal.org/dna-barcoding-and-species-identification-for-some-saudi-arabia-seaweeds-using-rbcl-gene/seaweedssaudi arabiarbclspecies identificationgeographical distribution |
| spellingShingle | Mohammed Ali Alshehri Al Thabiani Aziz Othman Alzahrani Abdulrahman Alasmari Shafik Ibrahim Gamal Osman Omar Bahattab DNA-barcoding and Species Identification for Some Saudi Arabia Seaweeds using rbcL Gene Journal of Pure and Applied Microbiology seaweeds saudi arabia rbcl species identification geographical distribution |
| title | DNA-barcoding and Species Identification for Some Saudi Arabia Seaweeds using rbcL Gene |
| title_full | DNA-barcoding and Species Identification for Some Saudi Arabia Seaweeds using rbcL Gene |
| title_fullStr | DNA-barcoding and Species Identification for Some Saudi Arabia Seaweeds using rbcL Gene |
| title_full_unstemmed | DNA-barcoding and Species Identification for Some Saudi Arabia Seaweeds using rbcL Gene |
| title_short | DNA-barcoding and Species Identification for Some Saudi Arabia Seaweeds using rbcL Gene |
| title_sort | dna barcoding and species identification for some saudi arabia seaweeds using rbcl gene |
| topic | seaweeds saudi arabia rbcl species identification geographical distribution |
| url | https://microbiologyjournal.org/dna-barcoding-and-species-identification-for-some-saudi-arabia-seaweeds-using-rbcl-gene/ |
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