Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (<i>Abelmoschus esculentus</i> L.)
Waterlogging stress adversely affects crop growth and yield worldwide. Effect of sodium azide priming on waterlogging stress tolerance of okra plants was investigated. The study was conducted as a field experiment using two weeks old plants grown from 0%, 0.02%, and 0.05% sodium azide (NaN<sub>...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-10-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/9/11/679 |
| _version_ | 1818321037111066624 |
|---|---|
| author | Emuejevoke D. Vwioko Mohamed A. El-Esawi Marcus E. Imoni Abdullah A. Al-Ghamdi Hayssam M. Ali Mostafa M. El-Sheekh Emad A. Abdeldaym Monerah A. Al-Dosary |
| author_facet | Emuejevoke D. Vwioko Mohamed A. El-Esawi Marcus E. Imoni Abdullah A. Al-Ghamdi Hayssam M. Ali Mostafa M. El-Sheekh Emad A. Abdeldaym Monerah A. Al-Dosary |
| author_sort | Emuejevoke D. Vwioko |
| collection | DOAJ |
| description | Waterlogging stress adversely affects crop growth and yield worldwide. Effect of sodium azide priming on waterlogging stress tolerance of okra plants was investigated. The study was conducted as a field experiment using two weeks old plants grown from 0%, 0.02%, and 0.05% sodium azide (NaN<sub>3</sub>)-treated seeds. The waterlogging conditions applied were categorized into control, one week, and two weeks. Different growth and reproductive parameters were investigated. Activity and expression of antioxidant enzymes, root anatomy, and soil chemical analysis were also studied. Results showed that sodium azide priming inhibited germination. The germination percentages recorded were 92.50, 85.00, and 65.00 for 0%, 0.02%, and 0.05% NaN<sub>3</sub>-treated seeds, respectively, nine days after planting. Waterlogging conditions depressed plant height ten weeks after planting. Under waterlogging conditions, NaN<sub>3</sub> promoted plant height and number of leaves formed. NaN<sub>3</sub> also supported the survival of plants and formation of adventitious roots under waterlogging conditions. Waterlogging conditions negatively affected the redox potential, organic C, N, and P concentrations in the soil but enhanced Soil pH, Fe, Mn, Zn, and SO<sub>4</sub>. Under waterlogging conditions, NaN<sub>3</sub> increased the average number of flower buds, flowers, and fruits produced in comparison to control. Moreover, NaN<sub>3</sub> highly stimulated the development of aerenchyma which in turn enhanced the survival of okra plants under waterlogging conditions. NaN<sub>3</sub> priming also enhanced the activities and gene expression level of antioxidant enzymes (ascorbate peroxidase, APX; catalase, CAT) under waterlogging conditions. In conclusion, this study demonstrated that NaN<sub>3</sub> priming could improve waterlogging stress tolerance in okra. |
| first_indexed | 2024-12-13T10:34:32Z |
| format | Article |
| id | doaj.art-102e5d4f85314b2996fb721f0bde5a29 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-12-13T10:34:32Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-102e5d4f85314b2996fb721f0bde5a292022-12-21T23:50:45ZengMDPI AGAgronomy2073-43952019-10-0191167910.3390/agronomy9110679agronomy9110679Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (<i>Abelmoschus esculentus</i> L.)Emuejevoke D. Vwioko0Mohamed A. El-Esawi1Marcus E. Imoni2Abdullah A. Al-Ghamdi3Hayssam M. Ali4Mostafa M. El-Sheekh5Emad A. Abdeldaym6Monerah A. Al-Dosary7Department of Plant Biotechnology, Faculty of Life Sciences, University of Benin, P.O. Box 1154, Benin City, NigeriaBotany Department, Faculty of Science, Tanta University, Tanta 31527, EgyptDepartment of Plant Biotechnology, Faculty of Life Sciences, University of Benin, P.O. Box 1154, Benin City, NigeriaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaBotany Department, Faculty of Science, Tanta University, Tanta 31527, EgyptVegetable Crops Department, Faculty of Agriculture, Cairo University, Giza P.O. Box 12613, EgyptBotany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaWaterlogging stress adversely affects crop growth and yield worldwide. Effect of sodium azide priming on waterlogging stress tolerance of okra plants was investigated. The study was conducted as a field experiment using two weeks old plants grown from 0%, 0.02%, and 0.05% sodium azide (NaN<sub>3</sub>)-treated seeds. The waterlogging conditions applied were categorized into control, one week, and two weeks. Different growth and reproductive parameters were investigated. Activity and expression of antioxidant enzymes, root anatomy, and soil chemical analysis were also studied. Results showed that sodium azide priming inhibited germination. The germination percentages recorded were 92.50, 85.00, and 65.00 for 0%, 0.02%, and 0.05% NaN<sub>3</sub>-treated seeds, respectively, nine days after planting. Waterlogging conditions depressed plant height ten weeks after planting. Under waterlogging conditions, NaN<sub>3</sub> promoted plant height and number of leaves formed. NaN<sub>3</sub> also supported the survival of plants and formation of adventitious roots under waterlogging conditions. Waterlogging conditions negatively affected the redox potential, organic C, N, and P concentrations in the soil but enhanced Soil pH, Fe, Mn, Zn, and SO<sub>4</sub>. Under waterlogging conditions, NaN<sub>3</sub> increased the average number of flower buds, flowers, and fruits produced in comparison to control. Moreover, NaN<sub>3</sub> highly stimulated the development of aerenchyma which in turn enhanced the survival of okra plants under waterlogging conditions. NaN<sub>3</sub> priming also enhanced the activities and gene expression level of antioxidant enzymes (ascorbate peroxidase, APX; catalase, CAT) under waterlogging conditions. In conclusion, this study demonstrated that NaN<sub>3</sub> priming could improve waterlogging stress tolerance in okra.https://www.mdpi.com/2073-4395/9/11/679sodium azideokrawaterlogging stressantioxidantsgene expression |
| spellingShingle | Emuejevoke D. Vwioko Mohamed A. El-Esawi Marcus E. Imoni Abdullah A. Al-Ghamdi Hayssam M. Ali Mostafa M. El-Sheekh Emad A. Abdeldaym Monerah A. Al-Dosary Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) Agronomy sodium azide okra waterlogging stress antioxidants gene expression |
| title | Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_full | Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_fullStr | Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_full_unstemmed | Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_short | Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_sort | sodium azide priming enhances waterlogging stress tolerance in okra i abelmoschus esculentus i l |
| topic | sodium azide okra waterlogging stress antioxidants gene expression |
| url | https://www.mdpi.com/2073-4395/9/11/679 |
| work_keys_str_mv | AT emuejevokedvwioko sodiumazideprimingenhanceswaterloggingstresstoleranceinokraiabelmoschusesculentusil AT mohamedaelesawi sodiumazideprimingenhanceswaterloggingstresstoleranceinokraiabelmoschusesculentusil AT marcuseimoni sodiumazideprimingenhanceswaterloggingstresstoleranceinokraiabelmoschusesculentusil AT abdullahaalghamdi sodiumazideprimingenhanceswaterloggingstresstoleranceinokraiabelmoschusesculentusil AT hayssammali sodiumazideprimingenhanceswaterloggingstresstoleranceinokraiabelmoschusesculentusil AT mostafamelsheekh sodiumazideprimingenhanceswaterloggingstresstoleranceinokraiabelmoschusesculentusil AT emadaabdeldaym sodiumazideprimingenhanceswaterloggingstresstoleranceinokraiabelmoschusesculentusil AT monerahaaldosary sodiumazideprimingenhanceswaterloggingstresstoleranceinokraiabelmoschusesculentusil |