Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus
In this study, cobalt phthalocyanine (CoPc) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) were designed and implemented to detect sequence-selective DNA hybridization related to the Hepatitis B virus (HBV). The surface characterization of CoPc-IL-PGEs was investigated by scanning...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2072-666X/12/7/753 |
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| author | Ece Yaralı Arzum Erdem |
| author_facet | Ece Yaralı Arzum Erdem |
| author_sort | Ece Yaralı |
| collection | DOAJ |
| description | In this study, cobalt phthalocyanine (CoPc) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) were designed and implemented to detect sequence-selective DNA hybridization related to the Hepatitis B virus (HBV). The surface characterization of CoPc-IL-PGEs was investigated by scanning electron microscopy (SEM), and the electrochemical behavior of electrodes were studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. The voltammetric detection of hybridization was investigated by evaluating the guanine oxidation signal, measured by differential pulse voltammetry (DPV) technique. The implementation of our biosensor to serum samples was also examined using fetal bovine serum (FBS). The detection limit was established as 0.19 µg/mL in phosphate buffer solution (PBS) (pH 7.40) and 2.48 µg/mL in FBS medium. The selectivity of our assay regarding HBV DNA hybridization in FBS medium was tested in the presence of other DNA sequences. With this aim, the hybridization of DNA probe with non-complementary (NC) or mismatched DNA sequence (MM), or in the presence of mixture samples containing DNA target NC (1:1) or DNA target MM (1:1), was studied based on the changes in guanine signal. |
| first_indexed | 2024-03-10T10:01:17Z |
| format | Article |
| id | doaj.art-102f467ba3844dfe9f79ecc605479f44 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T10:01:17Z |
| publishDate | 2021-06-01 |
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| series | Micromachines |
| spelling | doaj.art-102f467ba3844dfe9f79ecc605479f442023-11-22T01:53:17ZengMDPI AGMicromachines2072-666X2021-06-0112775310.3390/mi12070753Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B VirusEce Yaralı0Arzum Erdem1Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, Izmir 35100, TurkeyDepartment of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, Izmir 35100, TurkeyIn this study, cobalt phthalocyanine (CoPc) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) were designed and implemented to detect sequence-selective DNA hybridization related to the Hepatitis B virus (HBV). The surface characterization of CoPc-IL-PGEs was investigated by scanning electron microscopy (SEM), and the electrochemical behavior of electrodes were studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. The voltammetric detection of hybridization was investigated by evaluating the guanine oxidation signal, measured by differential pulse voltammetry (DPV) technique. The implementation of our biosensor to serum samples was also examined using fetal bovine serum (FBS). The detection limit was established as 0.19 µg/mL in phosphate buffer solution (PBS) (pH 7.40) and 2.48 µg/mL in FBS medium. The selectivity of our assay regarding HBV DNA hybridization in FBS medium was tested in the presence of other DNA sequences. With this aim, the hybridization of DNA probe with non-complementary (NC) or mismatched DNA sequence (MM), or in the presence of mixture samples containing DNA target NC (1:1) or DNA target MM (1:1), was studied based on the changes in guanine signal.https://www.mdpi.com/2072-666X/12/7/753cobalt phthalocyanineionic liquidvoltammetric biosensorHepatitis B viruspencil graphite electrodedifferential pulse voltammetry |
| spellingShingle | Ece Yaralı Arzum Erdem Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus Micromachines cobalt phthalocyanine ionic liquid voltammetric biosensor Hepatitis B virus pencil graphite electrode differential pulse voltammetry |
| title | Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus |
| title_full | Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus |
| title_fullStr | Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus |
| title_full_unstemmed | Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus |
| title_short | Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus |
| title_sort | cobalt phthalocyanine ionic liquid composite modified electrodes for the voltammetric detection of dna hybridization related to hepatitis b virus |
| topic | cobalt phthalocyanine ionic liquid voltammetric biosensor Hepatitis B virus pencil graphite electrode differential pulse voltammetry |
| url | https://www.mdpi.com/2072-666X/12/7/753 |
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