Isolation and Identification of Pennogenin Tetraglycoside from <i>Cestrum nocturnum</i> (Solanaceae) and Its Antifungal Activity against <i>Fusarium kuroshium</i>, Causal Agent of Fusarium Dieback
Antifungal assay-guided fractionation of the methanolic crude extract of <i>Cestrum nocturnum</i> (Solanaceae), popular known as ‘lady of the night’, led the isolation and identification of the steroidal saponin named pennogenin tetraglycoside, which was identified for the first time in...
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/6/1860 |
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| author | Erika Valencia-Mejía Yeli Y. León-Wilchez Juan L. Monribot-Villanueva Mónica Ramírez-Vázquez Israel Bonilla-Landa José A. Guerrero-Analco |
| author_facet | Erika Valencia-Mejía Yeli Y. León-Wilchez Juan L. Monribot-Villanueva Mónica Ramírez-Vázquez Israel Bonilla-Landa José A. Guerrero-Analco |
| author_sort | Erika Valencia-Mejía |
| collection | DOAJ |
| description | Antifungal assay-guided fractionation of the methanolic crude extract of <i>Cestrum nocturnum</i> (Solanaceae), popular known as ‘lady of the night’, led the isolation and identification of the steroidal saponin named pennogenin tetraglycoside, which was identified for the first time in this plant species by spectroscopic means. The crude extract, fractions and pennogenin tetraglycoside exhibited mycelial growth inhibition of <i>Fusarium solani</i> and <i>F. kuroshium</i>. <i>F. solani</i> is a cosmopolitan fungal phytopathogen that affects several economically important crops. However, we highlight the antifungal activity displayed by pennogenin tetraglycoside against <i>F. kuroshium</i>, since it is the first plant natural product identified as active for this phytopathogen. This fungus along with its insect symbiont known as Kuroshio shot hole borer (<i>Euwallacea kuroshio</i>) are the causal agents of the plant disease Fusarium dieback that affects more than 300 plant species including avocado (<i>Persea americana</i>) among others of ecological relevance. Scanning electron microscopy showed morphological alterations of the fungal hyphae after exposure with the active fractions and 12 phenolic compounds were also identified by mass spectrometry dereplication as part of potential active molecules present in <i>C. nocturnum</i> leaves. |
| first_indexed | 2024-03-09T13:10:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T13:10:16Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-1030334764bb43c4a938f1a1ce82a6e42023-11-30T21:42:20ZengMDPI AGMolecules1420-30492022-03-01276186010.3390/molecules27061860Isolation and Identification of Pennogenin Tetraglycoside from <i>Cestrum nocturnum</i> (Solanaceae) and Its Antifungal Activity against <i>Fusarium kuroshium</i>, Causal Agent of Fusarium DiebackErika Valencia-Mejía0Yeli Y. León-Wilchez1Juan L. Monribot-Villanueva2Mónica Ramírez-Vázquez3Israel Bonilla-Landa4José A. Guerrero-Analco5Laboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C. (INECOL)—Clúster Científico y Tecnológico BioMimic<sup>®</sup>, Carretera Antigua a Coatepec N. 351, Xalapa 91073, Veracruz, MexicoLaboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C. (INECOL)—Clúster Científico y Tecnológico BioMimic<sup>®</sup>, Carretera Antigua a Coatepec N. 351, Xalapa 91073, Veracruz, MexicoLaboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C. (INECOL)—Clúster Científico y Tecnológico BioMimic<sup>®</sup>, Carretera Antigua a Coatepec N. 351, Xalapa 91073, Veracruz, MexicoUnidad de Microscopía Avanzada, Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C. (INECOL)—Clúster Científico y Tecnológico BioMimic<sup>®</sup>, Carretera Antigua a Coatepec N. 351, Xalapa 91073, Veracruz, MexicoLaboratorio de Química Orgánica, Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C. (INECOL)—Clúster Científico y Tecnológico BioMimic<sup>®</sup>, Carretera Antigua a Coatepec N. 351, Xalapa 91073, Veracruz, MexicoLaboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C. (INECOL)—Clúster Científico y Tecnológico BioMimic<sup>®</sup>, Carretera Antigua a Coatepec N. 351, Xalapa 91073, Veracruz, MexicoAntifungal assay-guided fractionation of the methanolic crude extract of <i>Cestrum nocturnum</i> (Solanaceae), popular known as ‘lady of the night’, led the isolation and identification of the steroidal saponin named pennogenin tetraglycoside, which was identified for the first time in this plant species by spectroscopic means. The crude extract, fractions and pennogenin tetraglycoside exhibited mycelial growth inhibition of <i>Fusarium solani</i> and <i>F. kuroshium</i>. <i>F. solani</i> is a cosmopolitan fungal phytopathogen that affects several economically important crops. However, we highlight the antifungal activity displayed by pennogenin tetraglycoside against <i>F. kuroshium</i>, since it is the first plant natural product identified as active for this phytopathogen. This fungus along with its insect symbiont known as Kuroshio shot hole borer (<i>Euwallacea kuroshio</i>) are the causal agents of the plant disease Fusarium dieback that affects more than 300 plant species including avocado (<i>Persea americana</i>) among others of ecological relevance. Scanning electron microscopy showed morphological alterations of the fungal hyphae after exposure with the active fractions and 12 phenolic compounds were also identified by mass spectrometry dereplication as part of potential active molecules present in <i>C. nocturnum</i> leaves.https://www.mdpi.com/1420-3049/27/6/1860<i>Fusarium kuroshium</i>Fusarium diebackantifungal activity<i>Cestrum nocturnum</i>steroidal saponins |
| spellingShingle | Erika Valencia-Mejía Yeli Y. León-Wilchez Juan L. Monribot-Villanueva Mónica Ramírez-Vázquez Israel Bonilla-Landa José A. Guerrero-Analco Isolation and Identification of Pennogenin Tetraglycoside from <i>Cestrum nocturnum</i> (Solanaceae) and Its Antifungal Activity against <i>Fusarium kuroshium</i>, Causal Agent of Fusarium Dieback Molecules <i>Fusarium kuroshium</i> Fusarium dieback antifungal activity <i>Cestrum nocturnum</i> steroidal saponins |
| title | Isolation and Identification of Pennogenin Tetraglycoside from <i>Cestrum nocturnum</i> (Solanaceae) and Its Antifungal Activity against <i>Fusarium kuroshium</i>, Causal Agent of Fusarium Dieback |
| title_full | Isolation and Identification of Pennogenin Tetraglycoside from <i>Cestrum nocturnum</i> (Solanaceae) and Its Antifungal Activity against <i>Fusarium kuroshium</i>, Causal Agent of Fusarium Dieback |
| title_fullStr | Isolation and Identification of Pennogenin Tetraglycoside from <i>Cestrum nocturnum</i> (Solanaceae) and Its Antifungal Activity against <i>Fusarium kuroshium</i>, Causal Agent of Fusarium Dieback |
| title_full_unstemmed | Isolation and Identification of Pennogenin Tetraglycoside from <i>Cestrum nocturnum</i> (Solanaceae) and Its Antifungal Activity against <i>Fusarium kuroshium</i>, Causal Agent of Fusarium Dieback |
| title_short | Isolation and Identification of Pennogenin Tetraglycoside from <i>Cestrum nocturnum</i> (Solanaceae) and Its Antifungal Activity against <i>Fusarium kuroshium</i>, Causal Agent of Fusarium Dieback |
| title_sort | isolation and identification of pennogenin tetraglycoside from i cestrum nocturnum i solanaceae and its antifungal activity against i fusarium kuroshium i causal agent of fusarium dieback |
| topic | <i>Fusarium kuroshium</i> Fusarium dieback antifungal activity <i>Cestrum nocturnum</i> steroidal saponins |
| url | https://www.mdpi.com/1420-3049/27/6/1860 |
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