Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition
Abstract Lactic acid bacteria are industrially important microorganisms recognized for fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Fermentation conditions such as concentration of initial glucose in the culture, concentration of...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-11-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0519-6 |
| _version_ | 1818618478701051904 |
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| author | Majdiah Othman Arbakariya B. Ariff Helmi Wasoh Mohd Rizal Kapri Murni Halim |
| author_facet | Majdiah Othman Arbakariya B. Ariff Helmi Wasoh Mohd Rizal Kapri Murni Halim |
| author_sort | Majdiah Othman |
| collection | DOAJ |
| description | Abstract Lactic acid bacteria are industrially important microorganisms recognized for fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Fermentation conditions such as concentration of initial glucose in the culture, concentration of lactic acid accumulated in the culture, types of pH control strategy, types of aeration mode and different agitation speed had influenced the cultivation performance of batch fermentation of Pediococcus acidilactici. The maximum viable cell concentration obtained in constant fed-batch fermentation at a feeding rate of 0.015 L/h was 6.1 times higher with 1.6 times reduction in lactic acid accumulation compared to batch fermentation. Anion exchange resin, IRA 67 was found to have the highest selectivity towards lactic acid compared to other components studied. Fed-batch fermentation of P. acidilactici coupled with lactic acid removal system using IRA 67 resin showed 55.5 and 9.1 times of improvement in maximum viable cell concentration compared to fermentation without resin for batch and fed-batch mode respectively. The improvement of the P. acidilactici growth in the constant fed-batch fermentation indicated the use of minimal and simple process control equipment is an effective approach for reducing by-product inhibition. Further improvement in the cultivation performance of P. acidilactici in fed-bath fermentation with in situ addition of anion-exchange resin significantly helped to enhance the growth of P. acidilactici by reducing the inhibitory effect of lactic acid and thus increasing probiotic production. |
| first_indexed | 2024-12-16T17:22:14Z |
| format | Article |
| id | doaj.art-103253389a84407c9a5474ec9b61ea1a |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-16T17:22:14Z |
| publishDate | 2017-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-103253389a84407c9a5474ec9b61ea1a2022-12-21T22:23:09ZengSpringerOpenAMB Express2191-08552017-11-017111410.1186/s13568-017-0519-6Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibitionMajdiah Othman0Arbakariya B. Ariff1Helmi Wasoh2Mohd Rizal Kapri3Murni Halim4Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaBioprocessing and Biomanufacturing Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaAbstract Lactic acid bacteria are industrially important microorganisms recognized for fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Fermentation conditions such as concentration of initial glucose in the culture, concentration of lactic acid accumulated in the culture, types of pH control strategy, types of aeration mode and different agitation speed had influenced the cultivation performance of batch fermentation of Pediococcus acidilactici. The maximum viable cell concentration obtained in constant fed-batch fermentation at a feeding rate of 0.015 L/h was 6.1 times higher with 1.6 times reduction in lactic acid accumulation compared to batch fermentation. Anion exchange resin, IRA 67 was found to have the highest selectivity towards lactic acid compared to other components studied. Fed-batch fermentation of P. acidilactici coupled with lactic acid removal system using IRA 67 resin showed 55.5 and 9.1 times of improvement in maximum viable cell concentration compared to fermentation without resin for batch and fed-batch mode respectively. The improvement of the P. acidilactici growth in the constant fed-batch fermentation indicated the use of minimal and simple process control equipment is an effective approach for reducing by-product inhibition. Further improvement in the cultivation performance of P. acidilactici in fed-bath fermentation with in situ addition of anion-exchange resin significantly helped to enhance the growth of P. acidilactici by reducing the inhibitory effect of lactic acid and thus increasing probiotic production.http://link.springer.com/article/10.1186/s13568-017-0519-6Lactic acid bacteriaLactic acidExtractive fermentationFed-batch fermentationEnd-product inhibitionAnion exchange resin |
| spellingShingle | Majdiah Othman Arbakariya B. Ariff Helmi Wasoh Mohd Rizal Kapri Murni Halim Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition AMB Express Lactic acid bacteria Lactic acid Extractive fermentation Fed-batch fermentation End-product inhibition Anion exchange resin |
| title | Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition |
| title_full | Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition |
| title_fullStr | Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition |
| title_full_unstemmed | Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition |
| title_short | Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition |
| title_sort | strategies for improving production performance of probiotic pediococcus acidilactici viable cell by overcoming lactic acid inhibition |
| topic | Lactic acid bacteria Lactic acid Extractive fermentation Fed-batch fermentation End-product inhibition Anion exchange resin |
| url | http://link.springer.com/article/10.1186/s13568-017-0519-6 |
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